Which of the following statement about 2D gel electrophoresis is correct? (a) SDS gel electrophoresis and isoelectric focusing both separate native proteins (b) SDS gel electrophoresis and isoelectric focusing both […]
Tag: gel electorphoresis
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CSIR NET Previous Year Questions Gel Electrophoresis
Isoelectric Focusing-The separation of proteins is only based on charge
Which of the following statement about isoelectric focusing is correct (a) Proteins separated by isoelectric focusing cannot be tested for biological activity (b) Proteins separated by it can be tested […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS PAGE separate proteins based on their molecular weight
Which of the following statements about SDS PAGE is correct ? a) Wanted proteins can be tested for their biological activity after separation by SDS PAGE (b) Proteins are solubilised […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS-PAGE -Sodium Dodecyl Sulfate – Polyacrylamide Gel Electrophoresis
In SDS PAGE the protein sample is first (a) Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis (b) Fractionated by electrophoresis then treated with […]
CSIR NET Previous Year Questions Gel Electrophoresis
The electrophoresis technique that use isoelectric focusing is
The electrophoresis technique that use isoelectric focusing is (a) AGE (b) PFGE (c) 2D PAGE (d) SDS PAGE Correct answer: (C) 2D PAGE Explanation: Isoelectric focusing (IEF) is a technique […]
CSIR NET Previous Year Questions Gel Electrophoresis
Two dimensional gel electrophoresis is a technique for separating proteins
Two dimensional gel electrophoresis is a technique for separating proteins (A) Based on charge (B) Based on mass (C) Based on both charge and mass (D) Based on its pI […]
CSIR NET Previous Year Questions Gel Electrophoresis
APS (Ammonium persulphate) is used in SDS-PAGE
APS (Ammonium persulphate) is used in SDS-PAGE for (A) preventing oxidation (B) cross-linking (C) its role as a catalyst (D) free radical formation Correct answer: (D) Free radical formation Explanation: […]
Education
Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands
Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands of 35 kDa and 45 kDa. However, in a gel filtration chromatography, the same protein […]
Education
Separated the two proteins from the mixture one is monomeric
What method we should use to separated the two proteins from the mixture in which one is monomeric with MW of 10 kDa and other is of two Homodimer’s with […]
CSIR NET Previous Year Questions Gel Electrophoresis
An oligonucleotide bound to a protein migrates slower than the free oligonucleotide in a polyacrylamide gel
An oligonucleotide bound to a protein migrates slower than the free oligonucleotide in a polyacrylamide gel. If antibody specific to the oligonucleotide binding protein is bound to this complex, the […]
