The main advantage of discontinuous buffer system in SDS and native PAGE is (a) Conformation of protein is conserved (b) Constantly maintain the charge of proteins (c) Assist in migration […]
Tag: gel electorphoresis
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CSIR NET Previous Year Questions Gel Electrophoresis
Tracking Dye Used in SDS PAGE
The tracking dye used in SDS PAGE will be (a) Anionic (b) Cationic (c) Non ionic (d) Amphipathic The correct answer is: (a) Anionic Explanation: In SDS-PAGE, tracking dyes like […]
CSIR NET Previous Year Questions Gel Electrophoresis
Tracking Dye in SDS PAGE of Protein
Which of these is used as a tracking dye in SDS PAGE of protein (a) Bromo phenol blue (b) Xylene cyanol (c) Orange G (d) All of the above […]
CSIR NET Previous Year Questions Gel Electrophoresis
Advantage of Difference in Gel Electrophoresis (DiGE)
Which of these is an advantage of difference in gel electrophoresis (DiGE) compared to gel free approaches (a) More sensitive (b) Intact proteins allow detection of changes in protein modification […]
CSIR NET Previous Year Questions Gel Electrophoresis
Electrophoresis of Single Stranded DNA or RNA
In case of electrophoresis of single stranded DNA or RNA, which type of gels are used ? (a) Renaturing (b) Denaturing (c) The routine agarose gel (d) The routine polyacrylamide […]
CSIR NET Previous Year Questions Gel Electrophoresis
Amount of agarose added is more Higher agarose concentration
If the amount of agarose added is more, the molecule under analysis should have following characteristic (a) Small size (b) Large size (c) Size has no relation with amount of […]
CSIR NET Previous Year Questions Gel Electrophoresis
The charge present on the DNA backbone is negative
The charge present on the DNA backbone is negative. The force required to accelerate the molecules toward anode is directly proportional to no. of (a) Sugar molecules (b) Phosphate group […]
CSIR NET Previous Year Questions Gel Electrophoresis
Serum protein separated in agarose gel stained with Ponseau S stain
Serum protein is separated in 1% agarose gel, 25minutes at 100 volts, stained with Ponseau S protein stain. The separation of the protein is not optimal. What would you do […]
CSIR NET Previous Year Questions Gel Electrophoresis
Electroendosmotic flow disturb protein migration in the gel
In electrophoretic separation of proteins in an agarose gel, electroendosmotic flow occurs. This is a buffer flow that may disturb protein migration in the gel. Which of the following alternatives […]
CSIR NET Previous Year Questions Gel Electrophoresis
Total charge of a protein in native gel is determined by the pH
In an electrophoresis system, using a native gel (agarose) proteins are separated due to difference in total charge. What decides the charge of protein ? (a) Voltage (b) Buffer’s pH […]
