Translating DNA Sequence 3’-AAGTACTCT-5’ into a Tripeptide: Step-by-Step Analysis

To determine the tripeptide produced by the given DNA sequence, we need to follow the central dogma steps: transcription and translation.

Step 1: Determine the mRNA Sequence

• The given DNA sequence is the template strand (3’ to 5’):
  3’-AAGTACTCT-5’

• The mRNA is synthesized complementary to the template strand in the 5’ to 3’ direction:

  • DNA 3’-A A G T A C T C T-5’

  • mRNA 5’-U U C A U G A G A-3’

Step 2: Divide mRNA into Codons

Reading the mRNA from 5’ to 3’:
5’-UUC | AUG | AGA-3’

• Codon 1: UUC

• Codon 2: AUG

• Codon 3: AGA

Step 3: Translate Codons into Amino Acids

Using the standard genetic code:

Step 4: Final Tripeptide

• The tripeptide sequence is: Phe – Met – Arg

Summary Table of Options

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• DNA to mRNA transcription

• mRNA codon translation

• Genetic code table

• Tripeptide synthesis from DNA

• Protein translation process

• Codon to amino acid mapping

• Central dogma of molecular biology

• Reading frame in translation

• mRNA sequence determination

• Amino acid sequence prediction

Conclusion

The DNA sequence 3’-AAGTACTCT-5’ transcribes to mRNA sequence 5’-UUC AUG AGA-3’, which translates into the tripeptide Phenylalanine – Methionine – Arginine (Phe – Met – Arg).

Correct answer: (4) Phe – Met – Arg