SDS used in the electrophoresis of proteins- SDS coats the protein with a negative charge so that the sample can run through the gel

Why is SDS used in the electrophoresis of proteins?
a. SDS coats the protein with a negative charge so that the sample can run through the gel.
b. SDS is a specific protease that digests large proteins in the sample.
c. SDS allows the Coomassie blue stain to bind to the proteins in the gel so that they may be visualized.
d. SDS adds more molecular weight to each sample so that the proteins do not run off the end of the gel.

 

The correct answer is:

(a) SDS coats the protein with a negative charge so that the sample can run through the gel.

Explanation:

  • Sodium dodecyl sulfate (SDS) is a detergent used in SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) to denature proteins and give them a uniform negative charge.
  • This ensures that proteins are separated only by size, not by charge or shape.
  • In the presence of SDS, proteins migrate toward the positive electrode based on molecular weight, with smaller proteins moving faster.

Why Not the Other Options?

  • (b) SDS is a specific protease that digests large proteins in the sample
    Incorrect → SDS is a detergent, not a protease. Proteases (like trypsin or proteinase K) digest proteins, while SDS denatures them.

  • (c) SDS allows the Coomassie blue stain to bind to the proteins in the gel so that they may be visualized
    IncorrectCoomassie blue stain binds to proteins, but SDS does not play a role in staining. SDS only denatures and charges proteins.

  • (d) SDS adds more molecular weight to each sample so that the proteins do not run off the end of the gel
    Incorrect → SDS does not add significant molecular weight. Instead, it unfolds proteins and coats them with negative charge, allowing proper separation by size.

Conclusion:

SDS coats proteins with a negative charge, ensuring they migrate based on size in SDS-PAGE.
Thus, option (a) is correct.

85 Comments
  • Akshay mahawar
    February 25, 2025

    Done

  • Nisha
    February 25, 2025

    Done

    • Sneha kumawat s
      August 14, 2025

      Correct answer is A because sds denature protein and give them a negative charge

    • Mahima Sharma
      August 24, 2025

      Sds coat the protin with a beg charge on protein

  • Shreeji Charan
    February 26, 2025

    Was right

    • Aafreen
      August 14, 2025

      Ans-1
      SDS coats the protein with a negative charge so that the sample can run through the gel
      Bcoz proteins migrate toward the positive electrode based on molecular weight, with smaller proteins moving faster

  • Suman bhakar
    February 26, 2025

    Done

  • Parul
    February 26, 2025

    Done sir. Easy

  • Arushi
    February 26, 2025

    ☑️👍

  • Pooja jat
    February 27, 2025

    Done.

  • Lavanya Sharma
    February 27, 2025

  • Prami Masih
    February 27, 2025

    Okay sir

  • Ujjwal
    February 27, 2025

    ✔️✔️

  • yogesh sharma
    February 27, 2025

    Done sir

  • Abhilasha
    March 1, 2025

    Done

  • Lokesh kumawat
    March 1, 2025

    Done

  • VIKRAM GAHLOT
    March 7, 2025

    ok sir

  • Anita choudhary
    April 18, 2025

    Done ✅

  • Aman Choudhary
    August 13, 2025

    option a is correct protein par -ve charge aane se vo +ve electrode ki taraf jata h jisse vo molecular weight me bhi differentiate ho jate h low mw protein sabse neeche

  • Mohd juber Ali
    August 14, 2025

    Option (1)-SDS is detergent – and denature protein
    SDS separate protein on the basis of mass
    °Jab pH strip ko SDS solution me vartical rakhte h to negetively charge ho jati h
    SDS coats protein with negative charge

  • shruti sharma
    August 14, 2025

    option a is correct

  • Khushi Agarwal
    August 14, 2025

    Option 1 is correct
    Ye sb protein ke charge nagetive kr deta haii

  • Dharmpal Swami
    August 14, 2025

    Sds denatured protein and give uniformly negative charge

  • Anurag Giri
    August 14, 2025

    Sds gives uniform negative charge

  • Sneha kumawat
    August 14, 2025

    Correct answer is A because sds denature protein and give them a negative charge

    • Nilofar Khan
      August 16, 2025

      Correct answer is a

  • Sneha kumawat
    August 14, 2025

    Correct answer is A because sds denature protein and give them a negative charge .

  • Priya dhakad
    August 14, 2025

    SDS coats proteins with a negative charge, ensuring they migrate based on size in SDS-PAGE .option (a) is correct.

  • Soniya Shekhawat
    August 14, 2025

    SDS IS DETERGENT providing the uniform negative charge of all protein and separate on the basis of their molecular weight and size not a charge.

  • Mansukh Kapoor
    August 14, 2025

    The correct answer is option 1st .
    SDS gel mainly used in provide the uniform negative charge on the DNA which helps to run in the SDS page towards positive electrode

  • Anjani sharma
    August 14, 2025

    Answer 1 as sds provide uniform negative charge on the provide so that they can migrate towards + electrode

  • Neelam Sharma
    August 14, 2025

    SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Pratibha sethiya
    August 14, 2025

    Correct answer is 1
    SDS denature protein and give them a uniform negative charge

  • Dipti Sharma
    August 14, 2025

    Correct answer is 1
    SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Manisha gujar
    August 14, 2025

    Sds make negative charged protein which is helpful for run in gel

  • Karishma
    August 14, 2025

    SDS basically coats the protein with -ve charge

  • Priti khandal
    August 14, 2025

    yes sir option A is right because SDS coat the protein with a negative charge so that the sample and can Run through the gel

  • Priya khandal
    August 14, 2025

    Sds coats the protein with a negative charge so that the sample can run through gel

  • Roopal Sharma
    August 14, 2025

    SDS gives nehativer charge to protein

  • Santosh Saini
    August 15, 2025

    SDS provide the negative charge of IEF strip of a protein so that the sample can run through th gel

  • Shivani
    August 15, 2025

    Option a is correct .
    Sds provide negetive charge to the protein so that the sample can run through the gel

  • Payal
    August 17, 2025

    Sds provide negative charge on protein so protein move towards positive electrode and separation of protein bands according to weight

  • Manisha choudhary
    August 17, 2025

    Done sir
    Sds detergent h y protein ko denature kr deta h and uniformly negative charge provide krta h so protein kaa septation size k base p hoga shape and charge k base p nhi

  • Kavita choudhary
    August 17, 2025

    Right answer is a

  • Sheikh Aleen
    August 17, 2025

    Correct ans is a

  • Surbhi Rajawat
    August 17, 2025

    Option A is correct. Because SDS is a detergent which is used to provide extra negative charge to protein therefore it moves fastly in the gel

  • Kajal
    August 17, 2025

    SDS coats the protein with negative charge so they move fast in the gel

  • Avni
    August 17, 2025

    SDS provide negative charge to protein so that it can run through gel

  • Sakshi
    August 17, 2025

    a option is correct
    Because sds provide uniform negative charge to protein so it run towards positive charge

  • Khushi Vaishnav
    August 17, 2025

    SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Shrddha Modi
    August 17, 2025

    Sds protein ko equally negatively charge karta hai after breakdown so we can get same size of bands through this a is correct

  • Simran Saini
    August 18, 2025

    SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Meenakshi Choudhary
    August 19, 2025

    SDS coats proteins with a negative charge, ensuring they migrate based on size in SDS-PAGE.

  • Varsha Tatla
    August 19, 2025

    SDS provide -ve charge to protein so,protein sample move through the gel towards +ve electrod

  • Komal Sharma
    August 23, 2025

    Sodium dodecyl sulfate (SDS) is a detergent used in SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) to denature proteins and give them a uniform negative charge so option A is correct

  • Bhavana kankhedia
    August 24, 2025

    SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Bharti Yadav
    August 24, 2025

    SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Priyanka Choudhary
    August 24, 2025

    Correct answer is option A, because of this
    sds protein ko denature krta h and use nagetive charged provide krwata h

  • Priyanka Choudhary
    August 24, 2025

    Option a will be correct answer SDS coats the protein with a negative charge so that the sample can run through the gel

  • Urmi sisodiya
    August 24, 2025

    Sds used because it provides uniform charge on the molecules and ensure that the seperation is based on size not on charge

  • Sonam Saini
    August 24, 2025

    Sd s protein ko nagetive aavesh se Dhak deta Hai taki jal aasani se Gujar sake

  • Ankita Pareek
    August 24, 2025

    Sds is anionic detergent coats the protein with negative charge so the it runs in gel easily

  • Alec
    August 24, 2025

    option a – SDS coats the protein with negative charge so that it can run through the gel.

  • Niti Tanwar
    August 24, 2025

    Correct is option a

  • Anjali
    August 24, 2025

    because sds denature protein and give them a negative charge so ans is a

  • Falguni Prakash
    August 24, 2025

    A

  • Vijay kumar Meena
    August 24, 2025

    A done ✔️ 🙌

  • Devika
    August 25, 2025

    Sds coats protein with negative charge and the protein can run through gel towards positive electrode

  • Kashish yadav
    August 25, 2025

    Done sir

  • sakshi khatri
    August 25, 2025

    a is correct

  • Ayush Dubey
    August 25, 2025

    SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Monika meena
    August 27, 2025

    A option is correct -A.)SDS coats the protein with a negative charge so, that the sample can run through the gel

  • Surendra Doodi
    August 27, 2025

    Sodium dodecyl sulfate (SDS) is a detergent used in SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) to denature proteins and give them a uniform negative charge.

  • Pratibha Jain
    August 30, 2025

    Correct answer is option (1)
    SDS denature protein and give them a uniform negative charge

  • Sheikh Aleen
    August 30, 2025

    SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Anisha Beniwal
    August 30, 2025

    SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Vanshika Sharma
    August 30, 2025

    Sds coats the protiens with a negative charge so that sample can run through the gel

  • Akshita Jangid
    August 31, 2025

    SDS denature protein and give them a uniform negative charge

  • Shobha Kanwar
    August 31, 2025

    Sds denatured protein and give uniformly negative charge

  • Asha Gurzzar
    August 31, 2025

    A is correct because sds gives negative charge on molecules so that they ran in gel

  • Anisha jakhar
    September 3, 2025

    (a) SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Muskan Yadav
    September 3, 2025

    SDS coats the protein with a negative charge so that the sample can run through the gel.

  • Ajay Sharma
    September 4, 2025

    Sds gives negative charge to protein so it can run towards positively charged electrode

  • Somya Yadav
    September 5, 2025

    SDS coat the protein with negative charge, so that the sample can run on gel and protein gets separated on the basis of mol. Wt.

  • Aakansha sharma Sharma
    October 1, 2025

    The correct answer is:

    (a) SDS coats the protein with a negative charge so that the sample can run through the gel.

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