Why is SDS used in the electrophoresis of proteins?
a. SDS coats the protein with a negative charge so that the sample can run through the gel.
b. SDS is a specific protease that digests large proteins in the sample.
c. SDS allows the Coomassie blue stain to bind to the proteins in the gel so that they may be visualized.
d. SDS adds more molecular weight to each sample so that the proteins do not run off the end of the gel.
The correct answer is:
(a) SDS coats the protein with a negative charge so that the sample can run through the gel.
Explanation:
- Sodium dodecyl sulfate (SDS) is a detergent used in SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) to denature proteins and give them a uniform negative charge.
- This ensures that proteins are separated only by size, not by charge or shape.
- In the presence of SDS, proteins migrate toward the positive electrode based on molecular weight, with smaller proteins moving faster.
Why Not the Other Options?
-
(b) SDS is a specific protease that digests large proteins in the sample →
❌ Incorrect → SDS is a detergent, not a protease. Proteases (like trypsin or proteinase K) digest proteins, while SDS denatures them. -
(c) SDS allows the Coomassie blue stain to bind to the proteins in the gel so that they may be visualized →
❌ Incorrect → Coomassie blue stain binds to proteins, but SDS does not play a role in staining. SDS only denatures and charges proteins. -
(d) SDS adds more molecular weight to each sample so that the proteins do not run off the end of the gel →
❌ Incorrect → SDS does not add significant molecular weight. Instead, it unfolds proteins and coats them with negative charge, allowing proper separation by size.
Conclusion:
✅ SDS coats proteins with a negative charge, ensuring they migrate based on size in SDS-PAGE.
Thus, option (a) is correct.



85 Comments
Akshay mahawar
February 25, 2025Done
Nisha
February 25, 2025Done
Sneha kumawat s
August 14, 2025Correct answer is A because sds denature protein and give them a negative charge
Mahima Sharma
August 24, 2025Sds coat the protin with a beg charge on protein
Shreeji Charan
February 26, 2025Was right
Aafreen
August 14, 2025Ans-1
SDS coats the protein with a negative charge so that the sample can run through the gel
Bcoz proteins migrate toward the positive electrode based on molecular weight, with smaller proteins moving faster
Suman bhakar
February 26, 2025Done
Parul
February 26, 2025Done sir. Easy
Arushi
February 26, 2025☑️👍
Pooja jat
February 27, 2025Done.
Lavanya Sharma
February 27, 2025✅
Prami Masih
February 27, 2025Okay sir
Ujjwal
February 27, 2025✔️✔️
yogesh sharma
February 27, 2025Done sir
Abhilasha
March 1, 2025Done
Lokesh kumawat
March 1, 2025Done
VIKRAM GAHLOT
March 7, 2025ok sir
Anita choudhary
April 18, 2025Done ✅
Aman Choudhary
August 13, 2025option a is correct protein par -ve charge aane se vo +ve electrode ki taraf jata h jisse vo molecular weight me bhi differentiate ho jate h low mw protein sabse neeche
Mohd juber Ali
August 14, 2025Option (1)-SDS is detergent – and denature protein
SDS separate protein on the basis of mass
°Jab pH strip ko SDS solution me vartical rakhte h to negetively charge ho jati h
SDS coats protein with negative charge
shruti sharma
August 14, 2025option a is correct
Khushi Agarwal
August 14, 2025Option 1 is correct
Ye sb protein ke charge nagetive kr deta haii
Dharmpal Swami
August 14, 2025Sds denatured protein and give uniformly negative charge
Anurag Giri
August 14, 2025Sds gives uniform negative charge
Sneha kumawat
August 14, 2025Correct answer is A because sds denature protein and give them a negative charge
Nilofar Khan
August 16, 2025Correct answer is a
Sneha kumawat
August 14, 2025Correct answer is A because sds denature protein and give them a negative charge .
Priya dhakad
August 14, 2025SDS coats proteins with a negative charge, ensuring they migrate based on size in SDS-PAGE .option (a) is correct.
Soniya Shekhawat
August 14, 2025SDS IS DETERGENT providing the uniform negative charge of all protein and separate on the basis of their molecular weight and size not a charge.
Mansukh Kapoor
August 14, 2025The correct answer is option 1st .
SDS gel mainly used in provide the uniform negative charge on the DNA which helps to run in the SDS page towards positive electrode
Anjani sharma
August 14, 2025Answer 1 as sds provide uniform negative charge on the provide so that they can migrate towards + electrode
Neelam Sharma
August 14, 2025SDS coats the protein with a negative charge so that the sample can run through the gel.
Pratibha sethiya
August 14, 2025Correct answer is 1
SDS denature protein and give them a uniform negative charge
Dipti Sharma
August 14, 2025Correct answer is 1
SDS coats the protein with a negative charge so that the sample can run through the gel.
Manisha gujar
August 14, 2025Sds make negative charged protein which is helpful for run in gel
Karishma
August 14, 2025SDS basically coats the protein with -ve charge
Priti khandal
August 14, 2025yes sir option A is right because SDS coat the protein with a negative charge so that the sample and can Run through the gel
Priya khandal
August 14, 2025Sds coats the protein with a negative charge so that the sample can run through gel
Roopal Sharma
August 14, 2025SDS gives nehativer charge to protein
Santosh Saini
August 15, 2025SDS provide the negative charge of IEF strip of a protein so that the sample can run through th gel
Shivani
August 15, 2025Option a is correct .
Sds provide negetive charge to the protein so that the sample can run through the gel
Payal
August 17, 2025Sds provide negative charge on protein so protein move towards positive electrode and separation of protein bands according to weight
Manisha choudhary
August 17, 2025Done sir
Sds detergent h y protein ko denature kr deta h and uniformly negative charge provide krta h so protein kaa septation size k base p hoga shape and charge k base p nhi
Kavita choudhary
August 17, 2025Right answer is a
Sheikh Aleen
August 17, 2025Correct ans is a
Surbhi Rajawat
August 17, 2025Option A is correct. Because SDS is a detergent which is used to provide extra negative charge to protein therefore it moves fastly in the gel
Kajal
August 17, 2025SDS coats the protein with negative charge so they move fast in the gel
Avni
August 17, 2025SDS provide negative charge to protein so that it can run through gel
Sakshi
August 17, 2025a option is correct
Because sds provide uniform negative charge to protein so it run towards positive charge
Khushi Vaishnav
August 17, 2025SDS coats the protein with a negative charge so that the sample can run through the gel.
Shrddha Modi
August 17, 2025Sds protein ko equally negatively charge karta hai after breakdown so we can get same size of bands through this a is correct
Simran Saini
August 18, 2025SDS coats the protein with a negative charge so that the sample can run through the gel.
Meenakshi Choudhary
August 19, 2025SDS coats proteins with a negative charge, ensuring they migrate based on size in SDS-PAGE.
Varsha Tatla
August 19, 2025SDS provide -ve charge to protein so,protein sample move through the gel towards +ve electrod
Komal Sharma
August 23, 2025Sodium dodecyl sulfate (SDS) is a detergent used in SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) to denature proteins and give them a uniform negative charge so option A is correct
Bhavana kankhedia
August 24, 2025SDS coats the protein with a negative charge so that the sample can run through the gel.
Bharti Yadav
August 24, 2025SDS coats the protein with a negative charge so that the sample can run through the gel.
Priyanka Choudhary
August 24, 2025Correct answer is option A, because of this
sds protein ko denature krta h and use nagetive charged provide krwata h
Priyanka Choudhary
August 24, 2025Option a will be correct answer SDS coats the protein with a negative charge so that the sample can run through the gel
Urmi sisodiya
August 24, 2025Sds used because it provides uniform charge on the molecules and ensure that the seperation is based on size not on charge
Sonam Saini
August 24, 2025Sd s protein ko nagetive aavesh se Dhak deta Hai taki jal aasani se Gujar sake
Ankita Pareek
August 24, 2025Sds is anionic detergent coats the protein with negative charge so the it runs in gel easily
Alec
August 24, 2025option a – SDS coats the protein with negative charge so that it can run through the gel.
Niti Tanwar
August 24, 2025Correct is option a
Anjali
August 24, 2025because sds denature protein and give them a negative charge so ans is a
Falguni Prakash
August 24, 2025A
Vijay kumar Meena
August 24, 2025A done ✔️ 🙌
Devika
August 25, 2025Sds coats protein with negative charge and the protein can run through gel towards positive electrode
Kashish yadav
August 25, 2025Done sir
sakshi khatri
August 25, 2025a is correct
Ayush Dubey
August 25, 2025SDS coats the protein with a negative charge so that the sample can run through the gel.
Monika meena
August 27, 2025A option is correct -A.)SDS coats the protein with a negative charge so, that the sample can run through the gel
Surendra Doodi
August 27, 2025Sodium dodecyl sulfate (SDS) is a detergent used in SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) to denature proteins and give them a uniform negative charge.
Pratibha Jain
August 30, 2025Correct answer is option (1)
SDS denature protein and give them a uniform negative charge
Sheikh Aleen
August 30, 2025SDS coats the protein with a negative charge so that the sample can run through the gel.
Anisha Beniwal
August 30, 2025SDS coats the protein with a negative charge so that the sample can run through the gel.
Vanshika Sharma
August 30, 2025Sds coats the protiens with a negative charge so that sample can run through the gel
Akshita Jangid
August 31, 2025SDS denature protein and give them a uniform negative charge
Shobha Kanwar
August 31, 2025Sds denatured protein and give uniformly negative charge
Asha Gurzzar
August 31, 2025A is correct because sds gives negative charge on molecules so that they ran in gel
Anisha jakhar
September 3, 2025(a) SDS coats the protein with a negative charge so that the sample can run through the gel.
Muskan Yadav
September 3, 2025SDS coats the protein with a negative charge so that the sample can run through the gel.
Ajay Sharma
September 4, 2025Sds gives negative charge to protein so it can run towards positively charged electrode
Somya Yadav
September 5, 2025SDS coat the protein with negative charge, so that the sample can run on gel and protein gets separated on the basis of mol. Wt.
Aakansha sharma Sharma
October 1, 2025The correct answer is:
(a) SDS coats the protein with a negative charge so that the sample can run through the gel.