How can we pick a fragment of DNA from EtBr stained gel, How would you pick the fragment you are interested in?
(a) By digesting with an enzyme, which will give you fragments of size that you are interested in
(b) By probing the gel with a piece of the sample DNA (c)By digesting with multiple enzymes
(d)By performing a PCR
The correct answer is:
(a) By digesting with an enzyme, which will give you fragments of the size that you are interested in.
Explanation:
- Restriction enzyme digestion is used to cut DNA at specific sequences, producing fragments of known sizes.
- After digestion, agarose gel electrophoresis is performed to separate the fragments based on size.
- The gel is stained with ethidium bromide (EtBr) (or other intercalating dyes like SYBR Safe) to visualize DNA under UV light.
- To isolate a specific fragment, we:
- Identify the band corresponding to the desired size.
- Cut the gel at that location.
- Extract the DNA using a gel extraction kit or other purification methods.
Why Not the Other Options?
- (b) Probing the gel with a piece of sample DNA → This is used in Southern blotting, not for picking a DNA fragment from a gel.
- (c) Digesting with multiple enzymes → This can help refine fragment selection but is not the method for physically picking a fragment from a gel.
- (d) Performing a PCR → PCR amplifies DNA but does not help in picking a DNA fragment from a gel.
Thus, option (a) is the correct answer.
78 Comments
Akshay mahawar
February 25, 2025Done 👍
Shreeji Charan
February 26, 2025Didn’t knew the answer bt video explanation cleared it
Mahima Sharma
August 24, 2025Specific dna fragment ke liye gel ko probe ke sath hybrid krege
Suman bhakar
February 26, 2025My answer not correct but video se smjh aa gya
Jonu meena
February 26, 2025👍🏻
Arushi
February 26, 2025☑️👍
Pooja jat
February 27, 2025My ans. Is not appropriate ,, but through this video solution i got clear my all doubts .
Thank you sir, for explaining us in such a easy way.
Lavanya Sharma
February 27, 2025I did not get the question but the solution helped me to understand it .
Prami Masih
February 27, 2025Okay sir
Ujjwal
February 27, 2025✔️✔️👍
yogesh sharma
February 27, 2025Sir different answers in video and theory
Shreeji Charan
March 1, 2025✔️✔️
Lokesh kumawat
March 1, 2025Done from Video solution
Abhilasha
March 1, 2025✅
Nisha
March 1, 2025Such a supportive learning video
Manisha gujar
August 14, 2025Option b is correct probe used for pick dna fragment
Manisha gujar
August 14, 2025Option b is correct dna fragment ko pick krenge probe use kr ke
Priti khandal
March 3, 2025Okk sir
VIKRAM GAHLOT
March 7, 2025ok sir
shivani meena
March 16, 2025done
Kabeer Narwal
March 24, 2025👍
Anita choudhary
April 18, 2025👍👍
Mohd juber Ali
August 14, 2025Option (a)
Pcr only amplifies dna not picking dna fragment and probing gel used in southern blotting so only option a is right
shruti sharma
August 14, 2025read it
Khushi Agarwal
August 14, 2025I understood this question but not the answer because in the explanation the 1st option is given as correct but this asked after digest so id understand And in the video solution option 2 is mentioned by using probe but I think the probe detects it a bit, right?
Aafreen
August 14, 2025May be 2nd but 1st given as correct answer
Dp
August 14, 2025Write answer 2
Son
August 14, 2025By vedio solution gave proper correct explanation of question so.by using probe we can detect particular fragment of DNA fragments
Mitali
August 14, 2025The correct answer is:
(a) By digesting with an enzyme, which will give you fragments of the size that you are interested in.
Bharti Yadav
August 14, 2025Restriction enzymes are used to cut the specific fragment so by using enzyme will get desired fragment so option a is correct.
Ankit gujjar
August 14, 20251 is the correct answer bec enzyme breakdown the particles the fragments will appear of known length
Dipti Sharma
August 14, 2025By probing the gel with a piece of sample dna
Priti khandal
August 14, 2025Option b is righht answer sir By probing the gel with a piece of sample dna
Priti khandal
August 14, 2025Option b is righht answer sir By probing the gel with a piece of sample dna
Neelam Sharma
August 14, 2025Probing the gel with a piece of sample DNA and Southern blotting, tech. Use
Priya khandal
August 14, 2025By probinding the gel with apiece of the sample dna
Monika Meena
August 15, 2025By probing the gel with a peice of sample DNA b is correct answer 👍
Mahima Sharma
August 15, 2025Probe use karke dna pick karege
Priyanka choudhary
August 16, 2025Correct answer is option 1st
Shrddha Modi
August 17, 2025Option b through sourthan blotting
Divya rani
August 17, 2025Probe the gel with piece of dna is tge right answer
Kavita choudhary
August 17, 2025Right answer is option a
Sheikh Aleen
August 17, 2025Right ans is option B
Surbhi Rajawat
August 17, 2025Restriction enzymes cut DNA at a specific position therefore to obtain the desired DNA fragment we will need to cut the complete DNA at specific site ny Restriction nucleases
Khushi Vaishnav
August 17, 2025By digesting with an enzyme, which will give you fragments of the size that you are interested in.
Simran Saini
August 18, 2025By digesting with an enzyme, which will give you fragments of size that you are interested in.
Varsha Tatla
August 19, 2025Yes,we can pick the dna fragment by etbr .by the following steps
#digestion by restriction enz- known size dna produce
#gel electrophoresis -produce fragment seprate on the basis of size
#gel stain with etbr- dna fragment visualize under uv light
So, option 2 will be correct answer here
Meenakshi Choudhary
August 19, 2025Option b is correct answer
Komal Sharma
August 23, 2025Option B is correct by probing the gel
Bhavana Kankhedia
August 23, 2025by the digesting with an enzyme, which will give you fragments of size that you are interested in.
karishma don
August 24, 2025confused
Priyanka Choudhary
August 24, 2025By digesting with an enzyme, which will give you fragments of the size that you are interested in
Priyanka Choudhary
August 24, 2025A will be right answer digesting with an enzyme, which will give you fragments of the size that you are interested in.
Sonam Saini
August 24, 2025Right opetion a
Urmi sisodiya
August 24, 2025I think option 2 is correct
Priya dhakad
August 24, 2025By digesting with an enzyme, which will give you fragments of the size that you are interested in.
Anjali
August 24, 2025Option a is correct answer by digesting with an enzyme, which will give you fragments of the size that you are interested in.
Falguni Prakash
August 24, 2025Option A
Ankita Pareek
August 24, 2025By digesting with an enzyme which will give fragments of the size in which you are interested
Vijay kumar Meena
August 24, 2025B correct
Devika
August 25, 2025By digesting with enzyme which will give you the fragments you are interested in
sakshi khatri
August 25, 2025a is correct
Kashish yadav
August 25, 2025Ok sir
sakshi khatri
August 25, 2025done
Shivani Panwar
August 25, 2025Done sir 🌺✅
Ayush Dubey
August 25, 2025By digesting with an enzyme, which will give you fragments of the size that you are interested in.
Harish Kumar
August 26, 2025Btbr ke Karan hamen DNA ke band dikhai deti Hai hamen jis size ka chahie vah kat ke le sakte hain
Surendra Doodi
August 27, 2025By digesting with an enzyme, which will give you fragments of the size that you are interested in.
Pratibha Jain
August 30, 2025Correct answer is option (a)
By digesting with an enzyme which will give fragments of the size in which you are interested
Vanshika Sharma
August 30, 2025Restriction enzyme digestion cut the dna fragments of size in which you are interested
anjani sharma
August 30, 2025Answer a
By digesting with an enzyme, which will give you fragments of the size that you are interested in.
Akshita Jangid
August 31, 2025By digesting with an enzyme, which will give fragments of the size that needed.
Shobha Kanwar
August 31, 2025Option 1 is correct answer bcoz enzyme breakdown the particles the fragments will appear of known length
Asha Gurzzar
August 31, 2025According to me option b is correct,by using probe
Anisha jakhar
September 3, 2025(a) By digesting with an enzyme, which will give you fragments of the size that you are interested in.
Muskan Yadav
September 3, 2025By digesting with an enzyme, which will give you fragments of the size that you are interested in.
Ajay Sharma
September 4, 2025By digesting with an enzyme
Aakansha sharma Sharma
September 29, 2025(b) By probing the gel with a piece of the sample DNA