Which of the following approaches is used to measure the approximate mass of the purified protein complex and
to determine its subunit composition?
(a) Polyacrylamide gel electrophoresis and molecular cloning
(b) Ion-exchange chromatography and density-gradient centrifugation
(c)Genomics and recombinant DNA technology
(d)Gel-filtration chromatography and Western blotting
The correct answer is:
(d) Gel-filtration chromatography and Western blotting
Explanation:
-
Gel-filtration chromatography (Size-exclusion chromatography, SEC)
- Separates proteins based on size and approximate molecular weight.
- Larger protein complexes elute first, while smaller ones elute later due to differential movement through porous beads.
- Helps estimate the mass of a purified protein complex.
-
Western blotting
- Detects specific proteins using antibodies.
- Can help determine the subunit composition of a protein complex if individual subunits are resolved on a SDS-PAGE gel and probed with specific antibodies.
Why Not the Other Options?
-
(a) Polyacrylamide gel electrophoresis and molecular cloning →
- PAGE (SDS-PAGE or native PAGE) helps analyze protein size but is not the best method for determining the approximate mass of an intact protein complex.
- Molecular cloning is for gene manipulation, not protein mass analysis.
-
(b) Ion-exchange chromatography and density-gradient centrifugation →
- Ion-exchange chromatography separates proteins based on charge, not mass.
- Density-gradient centrifugation is more useful for nucleic acids or organelles rather than purified protein complexes.
-
(c) Genomics and recombinant DNA technology →
- These are used for studying genes and producing recombinant proteins but do not determine protein mass or subunit composition.
Conclusion:
Gel-filtration chromatography provides an estimate of protein complex mass, while Western blotting helps analyze subunit composition, making (d) the correct answer.
68 Comments
Akshay mahawar
February 25, 2025Done 👍
Suman bhakar
February 26, 2025Ok
Mahima Sharma
August 24, 2025Gel filtration chromatography provide an estimate of protein complexes mass and western blotting
Arushi
February 26, 2025👍👍
Parul
February 27, 2025Understood sir.
Pooja jat
February 27, 2025Done
Lavanya Sharma
February 27, 2025🙏🏻✅
Lavanya Sharma
February 27, 2025Nicely done and the solution given is also very nice .
Lavanya Sharma
February 27, 2025Did it sir!
Prami Masih
February 27, 2025Done sir
Nisha
February 27, 2025Understood
yogesh sharma
February 27, 2025Done sir
Shreeji Charan
March 1, 2025🙋♀️
Lokesh kumawat
March 1, 2025Done
Abhilasha
March 1, 2025Done
Ujjwal
March 3, 2025✔️✔️
VIKRAM GAHLOT
March 7, 2025👍
Monika meena
August 27, 2025Gel filtration chromatography and western blotting
Monika meena
August 27, 2025Gel filtration chromatography and western blotting D answer is correct 👍
Anita choudhary
April 18, 2025Okay 👍
Aman Choudhary
August 13, 2025option d is correct Gel-filtration chromatography and Western blotting bcz it separate protein on the basis of size it also known as size exclusion chromatography
Mohd juber Ali
August 14, 2025Option (4)
Size exclusion chromatography separate dna on the basis of size and m.w.of purifi protein complex. And western blot detect specific dna by using Ab and also determine subunit composition of protein complex
Khushi Agarwal
August 14, 2025Gel electrophoresis
Mansukh Kapoor
August 14, 2025The correct answer is option 4th Gel filtration chromatography also known as size exclusion chromatography that means it separates the Dna on the basis of size.
Western blotting is the technique used for detect the subunits of the protein
Aafreen
August 14, 2025Option 4 is correct Gel filtration chromatography and western blotting
It separates proteins based on size and approximate molecular weight
Manisha gujar
August 14, 2025Gel filter filteration and western blotting is correct
Neelam Sharma
August 14, 2025Gel-filtration chromatography and Western blotting
Priti khandal
August 14, 2025the correct answer d Because gel filtration chromatography provide an estimate protein complex Mass while western bloating helps analyse subunit composition
Santosh Saini
August 15, 2025Gel filtration chromatography and western blotting
Manisha choudhary
August 16, 2025Sds page and native page protein size btate h mass k liye sahi method nhi h
Ion exchange chromatography charge k base p separate krta h mass k base p nhi
Gel filtration chromatography s mass pta kr skte h and western blotting subunit composition btata h
Avni
August 17, 2025Gel filtration chromatography and western Blotting is the correct answer
Kajal
August 17, 2025Gel filtration chromatography and western blotting
Shrddha Modi
August 17, 2025Gel filtration chromatography use for Detect the mass and Western bloating technique use for differentiate sub units. 4th is correct
Varsha Tatla
August 19, 2025Clear, best approach for purify the protien on the basis of molecular weight n western blotting use in determination of protein subunit
Sneha .......
August 20, 2025Gel filtration chromatography and westran blotting
Sheikh Aleen
August 21, 2025Gel-filtration chromatography and Western blotting
Bhavana Kankhedia
August 24, 2025gel-filtration chromatography and western blotting.
Bharti Yadav
August 24, 2025Gel-filtration chromatography separate proteins on the basis of size and approximate mol.wt. and Western blotting detect specific proteins using Ab and determine subunit composition of proteins.
Payal Gaur
August 24, 2025Gel filtration chromatography and western blotting technique
Meenakshi Choudhary
August 24, 2025Gel-filtration chromatography provides an estimate of protein complex mass, while Western blotting helps analyze subunit composition.
Option d is correct
Priyanka Choudhary
August 24, 2025D is correct answer,Gel-filtration chromatography and Western blotting
Sonam Saini
August 24, 2025Ses is right
Niti Tanwar
August 24, 2025Gel filtration chrometography and Western blotting
Priya dhakad
August 24, 2025Gel-filtration chromatography provides an estimate of protein complex mass, while Western blotting helps analyze subunit composition.
Anjali
August 24, 2025Gel-filtration chromatography use for to detect the mass and Western blotting.
Falguni Prakash
August 24, 2025Gel filtration chromatography and western blotting
Sakshi
August 24, 2025Gel filtration chromatography and western bloating is correct
Vijay kumar Meena
August 24, 2025D ✔️
Devika
August 25, 2025Gel filtration chromatography and western blottin
Kashish yadav
August 25, 2025Option d
sakshi khatri
August 25, 2025done
Shivani Panwar
August 25, 2025Done sir 🌺✅
Ayush Dubey
August 25, 2025Gel-filtration chromatography and Western blotting
Komal Sharma
August 25, 2025Gel-filtration chromatography provides an estimate of protein complex mass, while Western blotting helps analyze subunit composition, making (d) the correct answer.
shruti sharma
August 25, 2025Gel-filtration chromatography use for to detect the mass and Western blotting
Pratibha Jain
August 30, 2025Correct answer is option (d)
Gel filtration chromatography use for Detect the mass and Western bloating technique use for differentiate sub units.
anjani sharma
August 30, 2025Answer d
Gel-filtration chromatography and Western blotting
Anisha Beniwal
August 30, 2025Gel-filtration chromatography provides an estimate of protein complex mass, while Western blotting helps analyze subunit composition, making
Akshita Jangid
August 31, 2025Gel-filtration chromatography and Western blotting
Meera Gurjar
August 31, 2025Gel filtration chromatography
Asha Gurzzar
August 31, 2025D is correct,gel filtration chromatography and western blotting
Neelam Sharma
September 1, 2025Gel-filtration chromatography provides an estimate of protein complex mass, while Western blotting helps analyze subunit composition, making (d) the correct answer
Anisha jakhar
September 3, 2025(d) Gel-filtration chromatography and Western blotting
Muskan Yadav
September 3, 2025Gel-filtration chromatography and Western blotting
Ajay Sharma
September 4, 2025Gel filtration for mass and western blotting for subunits detection (sds)
Somya Yadav
September 5, 2025Gel filtration chromatography and western blotting D answer is correct 👍
Neelam Sharma
September 9, 2025Gel-filtration chromatography and
Aakansha sharma Sharma
October 1, 2025The correct answer is:
(d) Gel-filtration chromatography and Western blotting