In an electrophoresis system, using a native gel (agarose) proteins are separated due to difference in total charge.
What decides the charge of protein ?
(a) Voltage
(b) Buffer’s pH
(c) Protein size
(d) Support medium
✅ Correct answer: (B) Buffer’s pH
Explanation:
The total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids.
- The net charge of a protein depends on its isoelectric point (pI), which is the pH at which the protein has no net charge.
- If the buffer pH is below the pI, the protein gains positive charge and moves toward the cathode (-).
- If the buffer pH is above the pI, the protein gains negative charge and moves toward the anode (+).
- Native gel electrophoresis (agarose or polyacrylamide) separates proteins based on charge and shape without denaturation, unlike SDS-PAGE.
Why Not the Other Options?
-
(A) Voltage ❌
- Voltage affects the speed at which proteins migrate but does not determine their charge.
-
(C) Protein size ❌
- In native gel electrophoresis, size may influence mobility, but charge is the primary factor.
- Size is the main factor in SDS-PAGE, not native gels.
-
(D) Support medium ❌
- The gel (agarose or polyacrylamide) acts as a matrix for separation but does not determine the protein’s charge.
Final Answer:
✅ (B) Buffer’s pH
79 Comments
Nisha
February 25, 2025Done
Mahima Sharma
August 24, 2025Buffer ph
Shreeji charan
February 26, 2025Jesi surrounding vesa charge
Suman bhakar
February 26, 2025Nice explanation
Parul
February 26, 2025Well understood.
Arushi
February 26, 2025☑️👍
Pooja jat
February 27, 2025✅️👍👍
Lavanya Sharma
February 27, 2025👍🏻
Prami Masih
February 27, 2025Very nice sir
yogesh sharma
February 27, 2025Well defined by sir
Ujjwal
February 27, 2025Nice explanation sir
yogesh sharma
February 27, 2025Done sir
Akshay mahawar
February 28, 2025Done 👍
Lokesh kumawat
February 28, 2025Done
Abhilasha
March 1, 2025Got it 👍🏻
Priya khandal
March 2, 2025Nice explanation sir samajh me aa gaya
VIKRAM GAHLOT
March 7, 2025ok sir
Monu
March 24, 2025,👍👍👍
Anurag Giri
August 14, 2025Ans b total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids
Khushi Agarwal
August 14, 2025Charge depend on ph
Aman Choudhary
August 14, 2025option B is correct
Because it depend on medium if the medium is highly acidic then charge is positive and the medium is highly basic then charge is negative
Sakshi
August 25, 2025Buffers ph decide charge on protein
Priya dhakad
August 14, 2025The total charge of a protein in native gel electrophoresis is determined by the pH of the buffer becoz protein contain positive and negative charge
Priya dhakad
August 14, 2025A protein in native gel electrophoresis is determined by the pH of the buffer becoz protein contain positive and negative charge
Anjani sharma
August 14, 2025Answer b buffers ph
Soniya Shekhawat
August 14, 2025Charge depend upon buffer pH .
Sneha Kumawat
August 14, 2025Charge depends upon the buffer’s pH
Priyanka choudhary
August 15, 2025Buffer-ph is correct answer 👍
Mansukh Kapoor
August 14, 2025The correct answer is option 2nd
Buffer pH decides the charge on the protein
Karishma Don
August 14, 2025it depends on buffer’s ph
shruti sharma
August 14, 2025buffer ph
Dipti Sharma
August 14, 2025(B) Buffer’s pH
Acidic pH +ve charge
Basic pH -ve charge
Neelam Sharma
August 14, 2025Buffer PH
Deepika Sheoran
August 14, 2025Charge depends upon the buffer PH.
Monika jangid
August 14, 2025Buffer ph
Priti khandal
August 14, 2025Buffer pg
Priti khandal
August 14, 2025Buffer ph
Komal Pareek
August 14, 2025Best Institute for CSIR NET Lifesciences.
Great Explanation
Priya khandal
August 14, 2025Buffer PH
Roopal Sharma
August 14, 2025Buffer ph
Mitali
August 15, 2025(B) Buffer’s pH
Shivani
August 15, 2025Buffer’s ph is the right answer
Divya rani
August 16, 2025Charge on protein is depend on pi so if buffer’s ph>pi Then protein is negatively charged and if ph<pi then protein is positively charged.
Aafreen
August 17, 2025Buffer’s PH
Payal Gaur
August 17, 2025Sepration of protein by charge is define by buffer’s ph.
Kavita choudhary
August 17, 2025Buffer pH
Sheikh Aleen
August 17, 2025Buffer pH
Kajal
August 17, 2025Buffer PH
Avni
August 17, 2025Buffer’s pH
Khushi Vaishnav
August 17, 2025Buffers ph
Simran Saini
August 18, 2025Buffer’s ph.
Meenakshi Choudhary
August 19, 2025Buffer’s pH
Komal Sharma
August 23, 2025The total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids option B is correct
Bharti Yadav
August 24, 2025total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids.
Priyanka Choudhary
August 24, 2025Correct answer is buffer ph
Bhavana kankhedia
August 24, 2025buffer pH
Urmi sisodiya
August 24, 2025Buffer pH because protein contain both positive and negative charge
Sonam Saini
August 24, 2025pH of the buffer
Alec
August 24, 2025option b – it depends on the ph of the buffer
Niti tanwar
August 24, 2025Separation of protein by charge -buffer’s pH
Falguni Prakash
August 24, 20252
Anjali
August 24, 2025Buffer’s ph
Ankita Pareek
August 24, 2025PH of buffer
Anju
August 24, 2025Ans : b
Protein carries both +ve and -ve charge amino acids
Kashish yadav
August 25, 2025Buffer’s ph
sakshi khatri
August 25, 2025done
Ayush Dubey
August 25, 2025Buffer’s pH
Devika
August 25, 2025Charge depend on buffers ph
Pratibha Jain
August 30, 2025Correct answer is option (B)
Total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids
Sheikh Aleen
August 30, 2025Buffer’s pH
Anisha Beniwal
August 31, 2025Buffer’s pH
Vanshika Sharma
August 31, 2025Total charge in native gel is decided by ph of buffer
Shobha Kanwar
August 31, 2025option B is correct Bcoz it depend on medium if the medium is highly acidic then charge is positive and the medium is highly basic then charge is negative
Anisha jakhar
September 3, 2025Correct answer: (B) Buffer’s pH
Muskan Yadav
September 3, 2025The total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids.
Ajay Sharma
September 4, 2025Buffers ph decide whether the protein is positively charged or negatively charged and if ph is equal to pi then the protein will be neutral
Somya Yadav
September 5, 2025Buffer ph
Meera Gurjar
September 13, 2025Buffer pH
Aakansha sharma Sharma
October 1, 2025Correct answer: (B) Buffer’s pH