Total charge of a protein in native gel is determined by the pH

In an electrophoresis system, using a native gel (agarose) proteins are separated due to difference in total charge.
What decides the charge of protein ?
(a) Voltage
(b) Buffer’s pH
(c) Protein size
(d) Support medium

 

Correct answer: (B) Buffer’s pH


Explanation:

The total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids.

  • The net charge of a protein depends on its isoelectric point (pI), which is the pH at which the protein has no net charge.
  • If the buffer pH is below the pI, the protein gains positive charge and moves toward the cathode (-).
  • If the buffer pH is above the pI, the protein gains negative charge and moves toward the anode (+).
  • Native gel electrophoresis (agarose or polyacrylamide) separates proteins based on charge and shape without denaturation, unlike SDS-PAGE.

Why Not the Other Options?

  • (A) Voltage

    • Voltage affects the speed at which proteins migrate but does not determine their charge.
  • (C) Protein size

    • In native gel electrophoresis, size may influence mobility, but charge is the primary factor.
    • Size is the main factor in SDS-PAGE, not native gels.
  • (D) Support medium

    • The gel (agarose or polyacrylamide) acts as a matrix for separation but does not determine the protein’s charge.

Final Answer:

(B) Buffer’s pH

79 Comments
  • Nisha
    February 25, 2025

    Done

    • Mahima Sharma
      August 24, 2025

      Buffer ph

  • Shreeji charan
    February 26, 2025

    Jesi surrounding vesa charge

  • Suman bhakar
    February 26, 2025

    Nice explanation

  • Parul
    February 26, 2025

    Well understood.

  • Arushi
    February 26, 2025

    ☑️👍

  • Pooja jat
    February 27, 2025

    ✅️👍👍

  • Lavanya Sharma
    February 27, 2025

    👍🏻

  • Prami Masih
    February 27, 2025

    Very nice sir

  • yogesh sharma
    February 27, 2025

    Well defined by sir

  • Ujjwal
    February 27, 2025

    Nice explanation sir

  • yogesh sharma
    February 27, 2025

    Done sir

  • Akshay mahawar
    February 28, 2025

    Done 👍

  • Lokesh kumawat
    February 28, 2025

    Done

  • Abhilasha
    March 1, 2025

    Got it 👍🏻

  • Priya khandal
    March 2, 2025

    Nice explanation sir samajh me aa gaya

  • VIKRAM GAHLOT
    March 7, 2025

    ok sir

  • Monu
    March 24, 2025

    ,👍👍👍

  • Anurag Giri
    August 14, 2025

    Ans b total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids

  • Khushi Agarwal
    August 14, 2025

    Charge depend on ph

  • Aman Choudhary
    August 14, 2025

    option B is correct
    Because it depend on medium if the medium is highly acidic then charge is positive and the medium is highly basic then charge is negative

    • Sakshi
      August 25, 2025

      Buffers ph decide charge on protein

  • Priya dhakad
    August 14, 2025

    The total charge of a protein in native gel electrophoresis is determined by the pH of the buffer becoz protein contain positive and negative charge

  • Priya dhakad
    August 14, 2025

    A protein in native gel electrophoresis is determined by the pH of the buffer becoz protein contain positive and negative charge

  • Anjani sharma
    August 14, 2025

    Answer b buffers ph

  • Soniya Shekhawat
    August 14, 2025

    Charge depend upon buffer pH .

  • Sneha Kumawat
    August 14, 2025

    Charge depends upon the buffer’s pH

    • Priyanka choudhary
      August 15, 2025

      Buffer-ph is correct answer 👍

  • Mansukh Kapoor
    August 14, 2025

    The correct answer is option 2nd
    Buffer pH decides the charge on the protein

  • Karishma Don
    August 14, 2025

    it depends on buffer’s ph

  • shruti sharma
    August 14, 2025

    buffer ph

  • Dipti Sharma
    August 14, 2025

    (B) Buffer’s pH
    Acidic pH +ve charge
    Basic pH -ve charge

  • Neelam Sharma
    August 14, 2025

    Buffer PH

  • Deepika Sheoran
    August 14, 2025

    Charge depends upon the buffer PH.

  • Monika jangid
    August 14, 2025

    Buffer ph

  • Priti khandal
    August 14, 2025

    Buffer pg

  • Priti khandal
    August 14, 2025

    Buffer ph

  • Komal Pareek
    August 14, 2025

    Best Institute for CSIR NET Lifesciences.
    Great Explanation

  • Priya khandal
    August 14, 2025

    Buffer PH

  • Roopal Sharma
    August 14, 2025

    Buffer ph

  • Mitali
    August 15, 2025

    (B) Buffer’s pH

  • Shivani
    August 15, 2025

    Buffer’s ph is the right answer

  • Divya rani
    August 16, 2025

    Charge on protein is depend on pi so if buffer’s ph>pi Then protein is negatively charged and if ph<pi then protein is positively charged.

  • Aafreen
    August 17, 2025

    Buffer’s PH

  • Payal Gaur
    August 17, 2025

    Sepration of protein by charge is define by buffer’s ph.

  • Kavita choudhary
    August 17, 2025

    Buffer pH

  • Sheikh Aleen
    August 17, 2025

    Buffer pH

  • Kajal
    August 17, 2025

    Buffer PH

  • Avni
    August 17, 2025

    Buffer’s pH

  • Khushi Vaishnav
    August 17, 2025

    Buffers ph

  • Simran Saini
    August 18, 2025

    Buffer’s ph.

  • Meenakshi Choudhary
    August 19, 2025

    Buffer’s pH

  • Komal Sharma
    August 23, 2025

    The total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids option B is correct

  • Bharti Yadav
    August 24, 2025

    total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids.

  • Priyanka Choudhary
    August 24, 2025

    Correct answer is buffer ph

  • Bhavana kankhedia
    August 24, 2025

    buffer pH

  • Urmi sisodiya
    August 24, 2025

    Buffer pH because protein contain both positive and negative charge

  • Sonam Saini
    August 24, 2025

    pH of the buffer

  • Alec
    August 24, 2025

    option b – it depends on the ph of the buffer

  • Niti tanwar
    August 24, 2025

    Separation of protein by charge -buffer’s pH

  • Falguni Prakash
    August 24, 2025

    2

  • Anjali
    August 24, 2025

    Buffer’s ph

  • Ankita Pareek
    August 24, 2025

    PH of buffer

  • Anju
    August 24, 2025

    Ans : b
    Protein carries both +ve and -ve charge amino acids

  • Kashish yadav
    August 25, 2025

    Buffer’s ph

  • sakshi khatri
    August 25, 2025

    done

  • Ayush Dubey
    August 25, 2025

    Buffer’s pH

  • Devika
    August 25, 2025

    Charge depend on buffers ph

  • Pratibha Jain
    August 30, 2025

    Correct answer is option (B)
    Total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids

  • Sheikh Aleen
    August 30, 2025

    Buffer’s pH

  • Anisha Beniwal
    August 31, 2025

    Buffer’s pH

  • Vanshika Sharma
    August 31, 2025

    Total charge in native gel is decided by ph of buffer

  • Shobha Kanwar
    August 31, 2025

    option B is correct Bcoz it depend on medium if the medium is highly acidic then charge is positive and the medium is highly basic then charge is negative

  • Anisha jakhar
    September 3, 2025

    Correct answer: (B) Buffer’s pH

  • Muskan Yadav
    September 3, 2025

    The total charge of a protein in native gel electrophoresis is determined by the pH of the buffer because proteins contain both positively and negatively charged amino acids.

  • Ajay Sharma
    September 4, 2025

    Buffers ph decide whether the protein is positively charged or negatively charged and if ph is equal to pi then the protein will be neutral

  • Somya Yadav
    September 5, 2025

    Buffer ph

  • Meera Gurjar
    September 13, 2025

    Buffer pH

  • Aakansha sharma Sharma
    October 1, 2025

    Correct answer: (B) Buffer’s pH

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