22. An intron in a yeast reporter gene carries a mutation in the splice site branch point (UACUAAC to UACA*AAC). To suppress the mutation, a library of point mutants of […]
Tag: Splicing
Tag: Splicing
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mRNA Export Required ATP | Unspliced mRNA Retention | Dbp5 Role as RNA helicase
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- June 4, 2025
- 7 Comments
21. Which one of the following statements about mRNA export from the nucleus is INCORRECT? (1) Cellular pre-rnRNAs with introns are generally not exported out of nucleus (2) Dbp5 is […]
Nuclear Retention and Export of Pre-mRNAs with Splice Site Mutations
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- June 3, 2025
- 9 Comments
20. In order to ensure that only fully processed mature mRNAs are allowed to be exported to cytosol, pre- mRNAs associated with snRNPs are retained in the nucleus. To demonstrate […]
Nucleus Export Spliced RNA associated with the poly A binding and cap binding complex
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- June 3, 2025
- 11 Comments
19. Which one of the following mRNAs is most likely to be exported out of the nucleus? (1) Spliced RNA associated with the poly A binding and cap binding complex. […]
Pre-mRNA Splicing | snRNPs and the Spliceosome
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- June 3, 2025
- 9 Comments
18. Pre-mRNAs are rapidly bound by snRNPs which carry out dual steps of splicing that removes the intron and joins the upstream and downstream exons. The following statements describe some […]
The Branch Point Sequence | Small Nuclear RNAs U2 and U6.
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- June 3, 2025
- 12 Comments
17. Which of following is reactive centre for splicing of exons during processing of m-RNA? (1) U1 and U5 (2) Branch point, U2 and U6 (3) Branch point, U4 and […]
Multiple mRNA Sizes from a Prokaryotic Gene in Transgenic Tobacco Plants
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- June 3, 2025
- 6 Comments
16. A 1257 bp genomic DNA sequence of a prokaryotic gene was cloned under a strong constitutive promoter along with a suitable polyA signal and used for development of transgenic […]
Eukaryotic Gene Expression: Processing of hn-RNA Yields mRNA
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- June 3, 2025
- 8 Comments
15. Regarding gene expression of eukaryotes, which statement is correct? (1) m-RNA and template DNA are has same sequence (2) m-RNA and protein synthesis can occur simultaneously (3) processing of […]
Role of Cyclin T1 in the Human P-TEFb Complex | Transcription Elongation
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- June 3, 2025
- 12 Comments
The Role of Cyclin T1 in the Human P-TEFb Complex: Key Regulator of Transcription Elongation 14. The positive transcription elongation factor. P-TEFb, is a Mufti-protein complex that plays an essential […]
Eukaryotic RNA Processing | Phosphorylation of the Ser 2 | Polyadenylation | Xrn 2 degrades RNA
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- June 3, 2025
- 9 Comments
13. Which one of the following statements about eukaryotic RNA processing is INCORRECT? (1) Termination of transcription occurs co- transcriptionally at the polyadenylation site. (2) Phosphorylation of the Ser 2 […]


