In SDS PAGE the protein sample is first (a) Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis (b) Fractionated by electrophoresis then treated with […]
Tag: SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis)
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CSIR NET Previous Year Questions Gel Electrophoresis
The electrophoresis technique that use isoelectric focusing is
The electrophoresis technique that use isoelectric focusing is (a) AGE (b) PFGE (c) 2D PAGE (d) SDS PAGE Correct answer: (C) 2D PAGE Explanation: Isoelectric focusing (IEF) is a technique […]
CSIR NET Previous Year Questions Gel Electrophoresis
APS (Ammonium persulphate) is used in SDS-PAGE
APS (Ammonium persulphate) is used in SDS-PAGE for (A) preventing oxidation (B) cross-linking (C) its role as a catalyst (D) free radical formation Correct answer: (D) Free radical formation Explanation: […]
Education
Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands
Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands of 35 kDa and 45 kDa. However, in a gel filtration chromatography, the same protein […]
Education
Separated the two proteins from the mixture one is monomeric
What method we should use to separated the two proteins from the mixture in which one is monomeric with MW of 10 kDa and other is of two Homodimer’s with […]
CSIR NET Previous Year Questions Gel Electrophoresis
An oligonucleotide bound to a protein migrates slower than the free oligonucleotide in a polyacrylamide gel
An oligonucleotide bound to a protein migrates slower than the free oligonucleotide in a polyacrylamide gel. If antibody specific to the oligonucleotide binding protein is bound to this complex, the […]
CSIR NET Previous Year Questions Gel Electrophoresis
Chymotrypsinogen in the native state and reduced unfolded sates is loaded- Gel-electrophoresis
Chymotrypsinogen in the native state and reduced unfolded sates is loaded in well A and B respectively. They are electrophoreses and stain, one of the following results is likely to […]
CSIR NET Previous Year Questions Gel Electrophoresis
Proteins A and B having a molecular weight of 16500 and 35400 move 1.3 cm and 4.6 cm
Proteins A and B having a molecular weight of 16500 and 35400 move 1.3 cm and 4.6 cm, respectively, when electrophoresed through a gel. What is the molecular weight of […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS Used in the Electrophoresis of Proteins
Why is SDS used in the electrophoresis of proteins? a. SDS coats the protein with a negative charge so that the sample can run through the gel. b. SDS is […]
