A mixture of Cytochrome-C (MW 11.7 kD) and Myoglobin (MW 17.2 KD) are to be separated by polyacrylamide gel electrophoresis. Their isoelectric pH (pI) values are 9.6 and 7.2 respectively. […]
Tag: SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis)
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CSIR NET Previous Year Questions Gel Electrophoresis
Determination of the molecular weight by molecular exclusion chromatography
A protein was purified to homogeneity. Determination of the molecular weight by molecular exclusion chromatography yields 60 kd. Chromatography in the presence of 6 M urea yields a 30-kd species. […]
CSIR NET Previous Year Questions Gel Electrophoresis
DNA replication with radio-labeled nucleotides, followed by urea-polyacrylamide gel electrophoresis (PAGE)
In an invitro experiment using radio-labeled nucleotides, a researcher is trying to analyze the possible products or DNA replication by resolving the products using urea-polyacrylamide gel electrophoresis. In one experimental […]
CSIR NET Previous Year Questions Gel Electrophoresis
To determine the Isoelectric point of a protein
To determine the isoelectric point of a protein, first establish that a gel: (a) Contains a denaturing detergent that can distribute uniform negative charges over the protein’s surface. (b) Exhibits […]
CSIR NET Previous Year Questions Gel Electrophoresis
Two-dimensional gel electrophoresis (2D-GE)
The first step in two-dimensional gel electrophoresis generates a series of protein bands by isoelectric focusing. In a second step a strip of this gel is turned 90 degrees, placed […]
CSIR NET Previous Year Questions Gel Electrophoresis
Determine the isoelectric point of a protein
To determine the isoelectric point of a protein, first establish that a gel: (a) Contains a denaturing detergent that can distribute uniform negative charges over the protein’s surface. (b) Exhibits […]
CSIR NET Previous Year Questions Gel Electrophoresis
To determine the Isoelectric point of a Protein, Isoelectric focusing
To determine the isoelectric point of a protein, first establish that a gel: (a) Contains a denaturing detergent that can distribute uniform negative charges over the protein’s surface. (b) Exhibits […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS-PAGE & Protein Separation Role of SDS Explained
By adding SDS (sodium dodecyl sulfate) during the electrophoresis of proteins, it is possible to: (a) Determine a protein’s isoelectric point. (b) Determine an enzyme’s specific activity. (c) Determine the […]
CSIR NET Previous Year Questions Gel Electrophoresis
Tracking Dye in SDS PAGE of Protein
Which of these is used as a tracking dye in SDS PAGE of protein (a) Bromo phenol blue (b) Xylene cyanol (c) Orange G (d) All of the above […]
CSIR NET Previous Year Questions Gel Electrophoresis
In Electrophoresis Protein Will Move Until
In electrophoresis, protein will move until (a) Its ph is greater than pi (b) Its ph is smaller than pi (c) Its ph is equal to pi (d) Pi is […]
