Two dimensional gel electrophoresis is a technique for separating proteins (A) Based on charge (B) Based on mass (C) Based on both charge and mass (D) Based on its pI […]
Tag: Polyacrylamide gel
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CSIR NET Previous Year Questions Gel Electrophoresis
APS (Ammonium persulphate) is used in SDS-PAGE
APS (Ammonium persulphate) is used in SDS-PAGE for (A) preventing oxidation (B) cross-linking (C) its role as a catalyst (D) free radical formation Correct answer: (D) Free radical formation Explanation: […]
Education
Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands
Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands of 35 kDa and 45 kDa. However, in a gel filtration chromatography, the same protein […]
Education
Separated the two proteins from the mixture one is monomeric
What method we should use to separated the two proteins from the mixture in which one is monomeric with MW of 10 kDa and other is of two Homodimer’s with […]
CSIR NET Previous Year Questions Gel Electrophoresis
An oligonucleotide bound to a protein migrates slower than the free oligonucleotide in a polyacrylamide gel
An oligonucleotide bound to a protein migrates slower than the free oligonucleotide in a polyacrylamide gel. If antibody specific to the oligonucleotide binding protein is bound to this complex, the […]
CSIR NET Previous Year Questions Gel Electrophoresis
Important for Gel Electrophoresis to work
Which of the following is important for gel electrophoresis to work? a. Negatively charged nucleic acids to migrate through the gel. b. Ethidium bromide to provide a means to visualize […]
