Western Blotting: A Key Technique for Protein Detection and Analysis 35. What are the techniques use to transferred protein onto nitrocellulose paper? a. Far western b. Southern blotting c. Northern […]
Tag: DOT BLOT
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CSIR NET Previous Year Questions on Blotting
Beta Rays and Their Transmission in Different Isotopes
Beta Rays and Their Transmission in Different Isotopes ß rays are not transmitted by which one of the isotopes given below? (A) 14C (B) 3H (C) 32P (D) 125 I […]
CSIR NET Previous Year Questions on Blotting
Radioactive Decay Calculation: Activity of one milli Curie of P-Labelled ATP After 60 Days
Radioactive Decay Calculation: Activity of one milli Curie of P-Labelled ATP After 60 Days Calculate the activity of one milli Curie of P-labelled ATP after 60 days. If the half […]
CSIR NET Previous Year Questions on Blotting
Optimizing Stringency in Wash Buffers for Hybridization Assays
Optimizing Stringency in Wash Buffers for Hybridization Assays How could increase the stringency of a wash buffer so as to allow the detection of only completely matched hybrids? a. Lower […]
CSIR NET Previous Year Questions on Blotting
Southern Blotting vs. Northern Blotting: Understanding the Differences
Southern Blotting vs. Northern Blotting: Understanding the Differences How southern blotting is different from northen blotting P. The target is DNA Q. The probe should be selected taking introns into […]
CSIR NET Previous Year Questions on Blotting
Understanding Nucleic Acid Probes and Hybridization
Understanding Nucleic Acid Probes and Hybridization What is true statement about a nucleic acid probe to hybridize a. both the probe and the sequence to which it hybridizes must be […]
CSIR NET Previous Year Questions on Blotting
Sequence of Steps in Western Blotting: Understanding the Process
Sequence of Steps in Western Blotting: Understanding the Process Sequence of steps in western blotting is P. Protein denaturation Q. Hydrogen peroxide reduction R. Primary antibody binging S. Transfer onto […]
CSIR NET Previous Year Questions on Blotting
Role of Reporter Molecules in DNA Hybridization Assays
Role of Reporter Molecules in DNA Hybridization Assays How reporter molecule function in a DNA hybridization assay? a. Enhances the stringency of the hybridization reaction b. Binds the target DNA […]
CSIR NET Previous Year Questions on Blotting
Understanding Hybridization in DNA Fingerprinting
Understanding Hybridization in DNA Fingerprinting The work ‘Hybridization’ in DNA finger printing means a. Pairing between the nucleotides of DNA sample with probe b. Pairing between the nucleotides of DNA […]
CSIR NET Previous Year Questions on Blotting
Southern Blotting for Polymorphism Detection in Fetal Cells
Southern Blotting for Polymorphism Detection in Fetal Cells A protein D is encoded by a gene, which is 5 Kb long and has three Hind Ill restriction enzyme sites. The […]
