Detecting Alpha-Amylase Gene Polymorphism: The Role of Southern Blotting
Alpha-amylase gene polymorphism is determined by which technique?
A. Southern blot
B. Slot blot
C. Dot blot
D. Northern blot
Which Technique Determines Alpha-Amylase Gene Polymorphism?
Genetic polymorphisms refer to variations in DNA sequences among individuals. These variations can be identified using different blotting techniques. The correct answer for detecting alpha-amylase gene polymorphism is:
✅ A. Southern Blot
Southern blotting is a DNA-based hybridization technique used to analyze gene polymorphisms, including structural changes, insertions, deletions, and restriction fragment length polymorphisms (RFLPs).
Why Is Southern Blotting Used for Gene Polymorphism?
- Targets DNA: Southern blotting detects variations in genomic DNA, making it suitable for studying polymorphisms.
- Restriction Enzyme Analysis: The DNA is digested with restriction enzymes to identify differences in fragment lengths due to genetic variations.
- Specific Hybridization: A labeled DNA probe hybridizes with the alpha-amylase gene, allowing visualization of polymorphic regions.
Steps of Southern Blotting for Gene Polymorphism Analysis:
-
DNA Extraction:
- Genomic DNA is extracted from cells or tissue samples.
-
Digestion with Restriction Enzymes:
- DNA is cut into fragments using specific restriction enzymes.
-
Gel Electrophoresis:
- The DNA fragments are separated by agarose gel electrophoresis.
-
Transfer to Nitrocellulose or Nylon Membrane:
- The DNA is transferred onto a solid membrane for further analysis.
-
Hybridization with a DNA Probe:
- A labeled complementary DNA probe binds to the alpha-amylase gene region.
-
Detection:
- The hybridized probe is detected using autoradiography or chemiluminescence to analyze polymorphisms.
Why the Other Options Are Incorrect:
❌ B. Slot Blot – Detects nucleic acids but does not provide fragment size information.
❌ C. Dot Blot – Similar to Slot Blot but lacks size resolution and cannot analyze polymorphisms.
❌ D. Northern Blot – Used for RNA analysis, not DNA polymorphism detection.
Conclusion:
Southern blotting remains a crucial technique for detecting alpha-amylase gene polymorphisms, helping researchers study genetic diversity, disease associations, and evolutionary patterns.
79 Comments
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March 3, 2025👍👍
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Nisha
March 4, 2025Done
Prami Masih
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Lokesh kumawat
March 4, 2025Done
VIKRAM GAHLOT
March 7, 2025👍
Neelam Sharma
August 19, 2025Southern blotting
Dipti Sharma
August 20, 2025Southern blotting
Khushi Mehra
August 20, 2025Gene polymorphism refers to the variation in DNA sequences among individuals
Southern blotting is used to detect specific DNA
Answer isA
Divya rani
August 21, 2025Gene polymorphism means variation in dna sequence and we detect the specific dna sequence By Southern blotting
Bharti Yadav
August 20, 2025Southern blotting
Mahima Sharma
August 21, 2025Southern blotting
Mansukh Kapoor
August 20, 2025The correct answer is option 1st
Southern blotting
Varsha Tatla
August 20, 2025Southern blotting
Sakshi yadav
August 20, 2025Southern blotting
Aman Choudhary
August 20, 2025Option A is correct
Southern blotting
Priya choudhary
August 20, 2025Option a is correct answer,this technique is used to detect specific DNA sequences in DNA samples
Surbhi Rajawat
August 20, 2025Gene polymorphism is the degree of variation in a gene in different individuals. Hence we have Southern blotting to determine the gene sequence.
anjani sharma
August 20, 2025Southern blotting
Aafreen
August 20, 2025Southern blotting is a DNA-based hybridization technique used to analyze gene polymorphisms
Kavita choudhary
August 20, 2025Sourthan blotting
Deepika Sheoran
August 20, 2025Option A is correct answer
Southern blotting
Mohd juber Ali
August 21, 2025(A) Southern blotting is right
labelled dna probe hybridize with alpha amylase (radiolabel complimentary dna probe binds with alpha amylase region )
Sneha .......
August 21, 2025Surthern blotting
Santosh Saini
August 21, 2025Southern blotting
Khushi Vaishnav
August 21, 2025Southern blotting is a DNA-based hybridization technique used to analyze gene polymorphisms
Simran Saini
August 21, 2025Southern blot.
Avni
August 21, 2025Southern blotting
Sakshi Kanwar
August 22, 2025Option A southern blotting because polymorphism is seen in DNA molecule as DNA POLYMERASE is error prone leads to changes and for DNA southern blotting is used
Anurag Giri
August 22, 2025(A) Southern blotting is right
labelled dna probe hybridize with alpha amylase (radiolabel complimentary dna probe binds with alpha amylase region )
Soniya Shekhawat
August 22, 2025Southern blotting is a technique for the hybridization of DNAby labelled probe used to analyse the gene polymorphism .
Arushi
August 22, 2025Southern blot
Kirti agarwal
August 23, 2025Southern blot
Priya dhakad
August 23, 2025Southern blotting is a DNA-based hybridization technique used to analyze gene polymorphisms so option is A
Priya dhakad
August 23, 2025Southern blotting is a DNA-based hybridization technique used to analyze gene polymorphisms so option is A correct
Priya khandal
August 23, 2025Southern blotting
Ankita Pareek
August 23, 2025Genetic polymorphism is the variation in dna sequence among individuals and out of given options Southern blotting is dna based hybridisation techniques which is used for gene polymorphism detection
karishma don
August 23, 2025Southern blotting is a DNA-based hybridization technique used to analyze gene polymorphisms, including RFLPs.
Kanishka Parnami
August 23, 2025Southern Blotting
Divya Mandhania
August 24, 2025Genetic polymorphism is the variation in DNAsequence and we detect the specific DN sequence by the technique southern toting so the correct answer is A
Devika
August 24, 2025Gene polymorphism is related to variations in dna sequences and for dna based hybridization we use Southern blotting
Yogita
August 24, 2025Southern blotting
Aakanksha Sharma
August 24, 2025Southern blotting
Himanshi
August 24, 2025Southern blotting
Himanshi
August 24, 2025Southern blot
Anisha Beniwal
August 24, 2025Southern blotting
AKANKSHA RAJPUT
August 24, 2025The correct answer is option (ONE )
Southern blotting
Shivani
August 24, 2025Alpha amylase gene polymorphism is determined by Southern blotting technique
Bhavana kankhedia
August 24, 2025Southern Blot
Kajal
August 24, 2025Southern blotting is a dna based hybrid isation technique used to analyse alpha amylase gene polymorphism.
Ayush
August 24, 2025Southern blotting
Priyanka Verma
August 24, 2025southern blotting is used to detect the alpha amylase gene polymorphism
Sonam Saini
August 24, 2025Southern bloting
Surendra Doodi
August 24, 2025Southern blotting
sakshi khatri
August 24, 2025southern blot
Falguni Prakash
August 24, 2025Southern blotting
Swati Choudhary
August 24, 2025Southern blotting is a DNA-based hybridization technique used to analyze gene polymorphisms so option is A.
Kajal
August 25, 2025Southern blott
Priyanka Meena
August 26, 2025Southern blotting
Muskan Yadav
August 26, 2025The correct answer for detecting alpha-amylase gene polymorphism is Southern blotting
Neha Yadav
August 27, 2025Southern blot
Manisha choudhary
August 27, 2025Gene m variations k liye southern blotting krenge gene part of DNA h and Southern blotting DNA hybridization technique h
Restriction endonuclease enzyme s cut krne p different size k fragments milte h jis s RFLP kaa pta lgta h
Slot blot nucliec acid k liye hota h pr different fragment size information nhi hota
Dot blot m bhi polymorphism kaa pta nhi lgta h
Komal Sharma
August 30, 2025Southern blotting is a DNA-based hybridization technique used to analyze gene polymorphisms, including structural changes, insertions, deletions, and restriction fragment length polymorphisms (RFLPs).
Shriyanshi verma
August 30, 2025Southern blotting
Alec
August 30, 2025Alpha amylase gene polymerization is detected by southern blotting.
Pratibha Jain
August 30, 2025Correct answer is option (A)
Southern Blot
Southern blotting is a DNA-based hybridization technique used to analyze gene polymorphisms, including structural changes, insertions, deletions, and restriction fragment length polymorphisms (RFLPs).
Anisha jakhar
August 31, 2025Southern blot
Shobha Kanwar
August 31, 2025Southern blotting is a DNA-based hybridization technique used to analyze gene polymorphisms.
Kanica Sunwalka
August 31, 2025s.blot used to analyze gene polymorphism
Anjali
September 1, 2025A is correct answer
Deepika sheoran
November 15, 2025Southern blotting