Southern Blotting: A Powerful Technique for DNA Fragment Detection
The Southern blotting technique is used for the detection of:
A. RNA fragmene on membranes by specific radioactive antibodies
B. DNA fragments on membranes by specific radioactive antibodies
C. DNA fragments on membranes by a radioactive DNA probe
D. Proteins on membranes using a radioactive DNA probe
What Does Southern Blotting Detect?
Southern blotting is a widely used molecular biology technique for detecting specific DNA sequences in a complex mixture. It allows researchers to identify genetic mutations, gene presence, and DNA polymorphisms.
The correct answer is:
✅ C. DNA fragments on membranes by a radioactive DNA probe
Why Is This the Correct Answer?
- Southern blotting detects DNA, not RNA or proteins.
- A DNA probe (radioactive or fluorescent-labeled) is used to hybridize with complementary DNA fragments on a membrane.
- This method is crucial in gene mapping, mutation analysis, and forensic studies.
Southern Blotting Process:
-
DNA Extraction & Digestion
- Genomic DNA is extracted and digested with restriction enzymes, cutting it into smaller fragments.
-
Agarose Gel Electrophoresis
- The DNA fragments are separated by size using agarose gel electrophoresis.
-
Denaturation & Transfer
- DNA is denatured into single strands and transferred onto a nitrocellulose or nylon membrane.
-
Hybridization with a Radioactive DNA Probe
- A labeled DNA probe binds to the target DNA sequence on the membrane.
-
Detection
- The hybridized DNA is visualized using autoradiography (if the probe is radioactive) or chemiluminescence.
Why the Other Options Are Incorrect:
❌ A. RNA fragments on membranes by specific radioactive antibodies
- This describes Northern blotting, which detects RNA, not DNA.
❌ B. DNA fragments on membranes by specific radioactive antibodies
- Southern blotting uses DNA probes, not antibodies.
❌ D. Proteins on membranes using a radioactive DNA probe
- This is incorrect because Western blotting detects proteins, but it does not use DNA probes.
Conclusion:
Southern blotting is a crucial method for detecting specific DNA fragments using a radioactive DNA probe. It remains a fundamental technique in genetics, diagnostics, and forensic investigations.
89 Comments
Suman bhakar
March 3, 2025Done
Mahima Sharma
August 21, 2025Fragment on membrane detected by dna prob
Ujjwal
March 3, 2025Okh sir
Akshay mahawar
March 3, 2025Easy👍
Arushi
March 3, 2025Done👍
Jyoti Meena
March 3, 2025Done
Ritika Jangir
March 3, 2025Done
Abhilasha
March 3, 2025Done
Priyam choudhary
March 3, 2025Okay 🆗
Priti khandal
March 3, 2025done👍
Nisha
March 4, 2025Done
Prami Masih
March 4, 2025Okay sir
Lokesh kumawat
March 4, 2025Done
VIKRAM GAHLOT
March 7, 2025done
Khushi Vaishnav
August 19, 2025DNA fragments on membranes by a radioactive DNA probe
Neelam Sharma
August 19, 2025DNA fragments on membranes by a radioactive DNA probe
Aakansha Sharma
August 20, 2025Option C
Dipti Sharma
August 20, 2025C. DNA fragments on membranes by a radioactive DNA probe
Khushi Mehra
August 20, 2025Southern blotting refers to the detection of specific DNA sequences on membrane by using radioactive DNA probe.
shruti sharma
August 20, 2025option c
Diksha Chhipa
August 20, 2025Done
Anurag Giri
August 20, 2025Ans c
Southern blotting is a crucial method for detecting specific DNA fragments using a radioactive DNA probe
Dharmpal Swami
August 20, 2025Dna fragments on membrane by specific radioactive probe
Bharti Yadav
August 20, 2025C. DNA fragments on membranes by a radioactive DNA probe
Nilofar Khan
August 20, 2025Dna fragments on membrane by a radioactive dna probe
Mansukh Kapoor
August 20, 2025The correct answer is option 3rd
Southern blotting determines the DNA fragments on membrane by using radioactive DNA probes
Sakshi yadav
August 20, 2025Option C
Varsha Tatla
August 20, 2025Option third will be correct 💯
Aman Choudhary
August 20, 2025Option c is correct
SB determine DNA fragments on membrane by radioactive DNA probe
Surbhi Rajawat
August 20, 2025Answer will be C because in Southern blotting we detect specific DNA sequence using radioactive probe.
Priya choudhary
August 20, 2025Option c is correct,dna fragments on membranes by a radioactive DNA probe
Priya dhakad
August 20, 2025DNA fragments on membranes by a radioactive DNA probe answer c is correct
anjani sharma
August 20, 2025Answer c DNA fragments on membranes by a radioactive DNA probe
Aafreen
August 20, 2025Ans-3
DNA fragments on membranes by a radioactive DNA probe
Kavita choudhary
August 20, 2025DNA fragments on membrane by a radioactive DNA probe
Neelam Sharma
August 20, 2025DNA fragment in membrane transfer and radioactive DNA prob used in southern blotting
Deepika Sheoran
August 20, 2025Answer Correct
DNA fragments on membrane by a Radio active DNA probe.
Manisha
August 21, 2025Southern blotting detection of DNA
Neha
August 21, 2025Dna fragments on membrane by radioactive dna probe
Payal Gaur
August 21, 2025DNA fragments on membrane by radio active DNA probe.
Sneha .......
August 21, 2025Dna fragments on membrane by radio active dna probe
Mohd juber Ali
August 21, 2025(C) southern blotting used to detect specific dna sequence on membrane by specific radiolabel dna probe
Santosh Saini
August 21, 2025Southern blotting detect to specific DNA by specific radioactive DNA probe
Meera Gurjar
August 21, 2025Fragments on membrane by radiolabelled DNA probe
Simran Saini
August 21, 2025DNA fragments on membrane by a radioactive DNA probe.
Avni
August 21, 2025Dna fragments on membrane by a radioactive DNA probe
Bhawna Choudhary
August 21, 2025DNA fragments on membrane by a radioactive DNA probe
Divya rani
August 21, 2025Detection of dna sequence on nitrocellulose membrane by using a radio labelled dna probe
Sakshi Kanwar
August 22, 2025Option C
Sneha Kumawat
August 22, 2025Southern blotting is used to specific dna sequence by using specific radio labelled probe
Soniya Shekhawat
August 22, 2025Southern blotting is used to detect a specific DNA sequence by radio labelled probe .
karishma
August 22, 2025southern blotting detects DNA fragments on membranes by a radioactive DNA probe
Arushi
August 22, 2025Radioactive DNA probes are used to detect DNA fragments on membrane
Kirti agarwal
August 23, 2025Opt c
Priya khandal
August 23, 2025DNA fragments on membrane by a radioactive DNA problem
Ankita Pareek
August 23, 2025Southern blotting detect specific dna on membrane by using radioactive dna probe
Niti Tanwar
August 23, 2025Right answer is c dna fragment on membrane
Akshita Jangid
August 23, 2025Southern blotting detects DNA fragments on a membrane by a radioactive DNA probe
Divya Mandhania
August 23, 2025C is right answer
Devika
August 24, 2025C.dna fragments on membrane by probe
Yogita
August 24, 2025DNA fragments on membrane by a radioactive DNA probe
Himanshi
August 24, 2025fragments on membranes by a radioactive DNA probe
Anisha Beniwal
August 24, 2025Option c is correct
AKANKSHA RAJPUT
August 24, 2025DONE SIR
Shivani
August 24, 2025Southen blotting is used to detect DNA fragments on membrane by using radioactive DNA probe
Kajal
August 24, 2025Dna fragments on membrane by radioactive probe
Bhavana kankhedia
August 24, 2025DNA fragments on membranes by a radioactive DNA probe
Ayush
August 24, 2025DNA fragments on membranes by a radioactive DNA probe
Tanvi Panwar
August 24, 2025Southern blotting is a technique used for detection of specific DNA by DNA probes.
Priyanka Verma
August 24, 2025southern blotting is used to detect the DNA fragments by radioactive probe
Sonam Saini
August 24, 2025Opetion c is Right
Surendra Doodi
August 24, 2025DNA fragments on membranes by a radioactive DNA probe
sakshi khatri
August 24, 2025c is correct
Falguni Prakash
August 24, 2025C
Swati Choudhary
August 24, 2025done
Kajal
August 25, 2025Dna fragments on membrane by radioactive dna probe
Priyanka Meena
August 26, 2025DNA fragments on membrane by a radioactive DNA
Muskan Yadav
August 26, 2025DNA fragments on membranes by a radioactive DNA probe.
Manisha choudhary
August 27, 2025Southern blotting gene identify krne gene mutations,DNA polymorphism k liye important h
Southern blotting m DNA probe use krte h antibody nhi
Probe radiolabeled h too detection k liye autoradiography krte h nhi too chemiluminiscene
Kanica Sunwalka
August 28, 2025option c
Komal Sharma
August 30, 2025DNA fragments on membranes by a radioactive DNA probe
Shriyanshi verma
August 30, 2025Fragment on membrane detected by dna prob
Alec
August 30, 2025Southern blotting is used to detect dna fragments on membrane by a radioactive probe.
Asha Gurzzar
August 30, 2025C is correct, southern blotting is used for specific dna detection
Pratibha Jain
August 30, 2025correct answer is option (C)
DNA fragments on membranes by a radioactive DNA probe
Anisha jakhar
August 31, 2025Option c
Shobha Kanwar
August 31, 2025Southern blotting refers to the detection of specific DNA sequences on membrane by using radioactive DNA probe
Anjali
September 1, 2025DNA fragments on membranes by a radioactive DNA probe . So correct answer is C
Deepika sheoran
November 15, 2025Option C is correct