If proteins are separated according to their electrophoretic mobility then type of electrophoresis is
(a) SDS PAGE
(b) Free flow electrophoresis
(c) Electro focusing
(d) Affinity electrophoresis
✅ Correct answer: (B) Free flow electrophoresis
Explanation:
Electrophoretic mobility is the rate at which a molecule moves in an electric field, which depends on factors like charge, size, and shape. Free-flow electrophoresis (FFE) is a specialized technique that separates proteins based on their electrophoretic mobility in a continuous buffer system.
Why is “Free-Flow Electrophoresis” the correct choice?
- Free-Flow Electrophoresis (FFE) separates proteins based on their electrophoretic mobility in a free-flowing liquid medium rather than a gel.
- It is useful for separating charged molecules in a continuous manner without the constraint of a gel matrix.
- Electrophoretic mobility depends on the charge-to-mass ratio of the protein in a given buffer system.
Why Not the Other Options?
-
(A) SDS-PAGE ❌
- SDS-PAGE separates proteins based on size, not electrophoretic mobility.
- SDS coats proteins with a uniform negative charge, meaning mobility depends only on molecular weight, not inherent charge.
-
(C) Electro-focusing (IEF) ❌
- Isoelectric focusing (IEF) separates proteins based on their isoelectric point (pI), not their general electrophoretic mobility.
-
(D) Affinity electrophoresis ❌
- Affinity electrophoresis separates proteins based on specific interactions with ligands (e.g., antigen-antibody binding) rather than electrophoretic mobility.
Final Answer:
✅ (B) Free flow electrophoresis
67 Comments
Nisha
February 25, 2025Done
Shreeji Charan
February 26, 2025Sir ye puri tarah samaj nhi aaya na iska video explanation dikh rha h
Pooja jat
February 27, 2025I think the ans . Is A.,, but in solutions its ans is b .
Question not understood.
Suman bhakar
February 26, 2025Video se smjh aa gya sir
Parul
February 26, 2025Done sir.
Arushi
February 26, 2025✔️
Arushi
February 26, 2025☑️☑️
Lavanya Sharma
February 27, 2025✅
Prami Masih
February 27, 2025Okay sir
yogesh sharma
February 27, 2025Sir please attach video for explanation
Ujjwal
February 27, 2025✔️✔️
yogesh sharma
February 27, 2025Sir video solution is not attached
Lokesh kumawat
February 28, 2025Required video solution
VIKRAM GAHLOT
March 7, 2025👍
Mohd juber Ali
August 14, 2025Option (B)
Soniya Shekhawat
August 14, 2025SDS is basis of size, affinty basis of antigen antibody interaction and IEF based on isoelectric point and charge so answer is free flow electrophoresis.
Mansukh Kapoor
August 14, 2025The correct answer is option 2nd because in free flow electrophoresis the freely movement of charge particles in the gel
shruti sharma
August 14, 2025ans is free flow electrophoresis
Karishma Don
August 14, 2025Free flow electrophoresis is the ans as SDS-PAGE separates on the basis of size, IEF SEPARATES ON THE BASIS OF pI.
Neelam Sharma
August 14, 2025Free flow electrophoresis
Deepika Sheoran
August 14, 2025Option b is correct answer
Free flow electrophoresis.
Priti khandal
August 14, 2025Free flow electrophoresis
Priya khandal
August 14, 2025Free Flow electrophoresis
Roopal Sharma
August 14, 2025Done
Priyanka choudhary
August 15, 2025Free flow electrophoresis is right answer 👍
Mitali
August 15, 2025(B) Free flow electrophoresis
Divya rani
August 16, 2025Free flow means liquid medium buffer and it is based on electrophoretic mobility
Mitali saini
August 15, 2025(B) Free flow electrophoresis
Shivani
August 15, 2025Free flow electrophoresis is the right answer
Heena
August 16, 2025Free flow electrophoresis
Aafreen
August 16, 2025Free Flow Electrophoresis
Kajal
August 17, 2025Free flow electrophoresis
Avni
August 17, 2025free flow electrophoresis
Khushi Vaishnav
August 17, 2025Free flow electrophoresis
Simran Saini
August 18, 2025Free flow electrophoresis.
Meenakshi Choudhary
August 19, 2025Free flow electrophoresis
Varsha tatla
August 19, 2025Correct answer will be free Flow electrophoresis
Manisha choudhary
August 20, 2025Free flow electrophoresis m protein electromobaility k base p Free flowing liquid medium m bina kisi gel ki badha k continuous sepration hota h
Sds page m mass k base p hota h
Electro focusing m isoelectric point k base p separate hota h
Affinity Electrophoresis m ligant specific binding k through isolate kiya jata h
Komal Sharma
August 23, 2025Electrophoretic mobility is the rate at which a molecule moves in an electric field, which depends on factors like charge, size, and shape. Free-flow electrophoresis (FFE) is a specialized technique that separates proteins based on their electrophoretic mobility in a continuous buffer system so option B is correct
Bhavana kankhedia
August 24, 2025free flow electrophoresis.
Bharti Yadav
August 24, 2025Free flow electrophoresis
Priyanka Choudhary
August 24, 2025Correct answer will we option b,Free flow electrophoresis
Akshita Jangid
August 24, 2025Free flow electrophoresis
Sonam Saini
August 24, 2025Free flow eracophosis
Alec
August 24, 2025option b – it is free flow electrophoresis.
Priya dhakad
August 24, 2025The separates proteins based on their electrophoretic mobility in a continuous buffer system.
Niti Tanwar
August 24, 2025B is correct – free flow electrophoresis
Falguni Prakash
August 24, 2025B
Sakshi
August 24, 2025Free flow electrophoresis is correct option
Kashish yadav
August 25, 2025Free flow electrophoresis
sakshi khatri
August 25, 2025done
Vijay kumar Meena
August 25, 2025Done ✔️
Ayush Dubey
August 25, 2025Free flow electrophoresis
Devika
August 25, 2025Free flow electrophoresis separates molecule on the basis of their electrophoretic mobility
Anju
August 25, 2025Ans:b
FFE technique that separate proteins on the bases of their electrophoretic mobility
Surendra Doodi
August 27, 2025Correct answer-B Free flow electrophoresis
Pratibha Jain
August 30, 2025Correct answer is option (B) Free flow electrophoresis
Sheikh Aleen
August 30, 2025Free flow electrophoresis
Anisha Beniwal
August 30, 2025Free flow electrophoresis
anjani sharma
August 30, 2025Answer b
Free flow electrophoresis
Vanshika Sharma
August 31, 2025Free flow electrophoresis
Shobha Kanwar
August 31, 2025Free flow means liquid medium buffer and it is based on electrophoretic mobility
Akshita Jangid
September 1, 2025Free flow electrophoresis technique separate proteins on the basis of their electrophoretic mobility
Asha Gurzzar
September 1, 2025B is correct free flow electrophoresis
Muskan Yadav
September 3, 2025Free flow electrophoresis
Somya Yadav
September 5, 2025Free flow electrophoresis
Aakansha sharma Sharma
October 1, 2025SDS is basis of size, affinty basis of antigen antibody interaction and IEF based on isoelectric point and charge so answer is free flow electrophoresis.