The electrophoresis technique that use isoelectric focusing is
(a) AGE
(b) PFGE
(c) 2D PAGE
(d) SDS PAGE
Correct answer: (C) 2D PAGE
Explanation:
Isoelectric focusing (IEF) is a technique used in Two-Dimensional Gel Electrophoresis (2D-PAGE) as the first dimension to separate proteins based on their isoelectric point (pI).
Why 2D-PAGE Uses Isoelectric Focusing?
-
First Dimension: Isoelectric Focusing (IEF)
- Proteins migrate in a pH gradient until they reach their pI (isoelectric point), where they have no net charge and stop moving.
- This separates proteins based on charge.
-
Second Dimension: SDS-PAGE
- After IEF, proteins are further separated using SDS-PAGE, which sorts them by molecular mass.
Thus, IEF is a key step in 2D-PAGE, making it the correct answer.
Why Not the Other Options?
-
(A) AGE (Agarose Gel Electrophoresis) ❌
- Used for DNA, RNA, or large proteins, but it does not involve isoelectric focusing.
-
(B) PFGE (Pulsed-Field Gel Electrophoresis) ❌
- A special type of gel electrophoresis for separating large DNA molecules, but it does not use IEF.
-
(D) SDS-PAGE ❌
- SDS-PAGE separates proteins by size only and does not involve charge-based separation like IEF.
Final Answer:
✅ (C) 2D PAGE
88 Comments
Nisha
February 25, 2025Done
Mahima Sharma
August 24, 20252D page
Shreeji Charan
February 26, 2025Was right
Suman bhakar
February 26, 2025Was right
Aa
February 26, 2025😍😍
Parul
February 26, 2025Easy one. Understood sir
Parul
February 26, 2025Easily understood sir.
Arushi
February 26, 2025☑️
Pooja jat
February 27, 2025Easy one .
Lavanya Sharma
February 27, 2025This was easy.
Lavanya Sharma
February 27, 2025✅👍🏻
Prami Masih
February 27, 2025Okay sir
yogesh sharma
February 27, 2025Before explanation 👹
After explanation 🤡
Ujjwal
February 27, 2025✔️👍
yogesh sharma
February 27, 2025Done sir
Lokesh kumawat
February 28, 2025Done
Abhilasha
March 1, 2025Right
Priya khandal
March 2, 2025👍
VIKRAM GAHLOT
March 7, 2025👍
Anita choudhary
April 18, 2025👍
Aman Choudhary
August 13, 2025option c is correct 2D PAGE it separate protein on the basis of charge and molecular weight
Mohd juber Ali
August 14, 2025Option(c) 2D gel electrophoresis techniques
Khushi Agarwal
August 14, 2025Option c is correct
2d page
Anurag Giri
August 14, 2025Ans c 2D page
Dharmpal Swami
August 14, 20252d page
Priya dhakad
August 14, 20252D PAGE
Neelam Sharma
August 14, 20252D PAGE
Anjani sharma
August 14, 20252D PAGE
Sneha Kumawat
August 14, 2025Correct answer is 2D page
Devika
August 14, 20252D page
shruti sharma
August 14, 20252D PAGE
Mansukh Kapoor
August 14, 2025The correct answer is option 3rd
2D PAGE separates the protein in IEF on the basis of iso electric point
Soniya Shekhawat
August 14, 2025Easy 😁
Dipti Sharma
August 14, 2025(C) 2D PAGE
Nilofar Khan
August 14, 2025Correct answer is (c) 2D PAGE
Karishma Don
August 14, 20252D PAGE- 1st IEF and 2nd SDS-PAGE
Priti khandal
August 14, 20252 D page
Komal Pareek
August 14, 20252d page
Priya khandal
August 14, 20252D page
Roopal Sharma
August 14, 20252D PAGE electrophoresis technique use the isoelectric focusing
Deepika Sheoran
August 15, 2025Option C is correct answer
2D PAGE
Priyanka choudhary
August 15, 20252D page was correct answer 👍
Shivani
August 15, 2025(c). 2D page
Mitali
August 16, 2025Correct answer: (C) 2D PAGE
Divya rani
August 16, 20252D = IEF+SDS Page
Aafreen
August 16, 20252-D PAGE
Varsha tatla
August 19, 20252d page
Kavita choudhary
August 17, 20252-D PAGE
Kajal
August 17, 20252 D phase
Avni
August 17, 2025(C) 2D PAGE
Sakshi
August 17, 20252d page is correct option
Khushi Vaishnav
August 17, 2025Isoelectric focusing (IEF) is a technique used in Two-Dimensional Gel Electrophoresis (2D-PAGE)
Simran Saini
August 18, 20252D PAGE.
Devika
August 25, 20252D PAGE
Meenakshi Choudhary
August 19, 20252D PAGE
Varsha tatla
August 19, 2025Easily solved it
Manisha choudhary
August 20, 2025Agarose gel electrophoresis m size and shape k base p separate kiya jata h DNA ,rna and large protein molecules ko
PFGE pulse field gel electrophoresis m large DNA ko chromosomes ko separate kiya jata h
Sds page size k base p separate hota h charge k base p nhi
Isoelectric focusing,2D PAGE m size ,charge k base p separate hota h
Komal Sharma
August 23, 2025Isoelectric focusing (IEF) is a technique used in Two-Dimensional Gel Electrophoresis (2D-PAGE) as the first dimension to separate proteins based on their isoelectric point (pI) so answer is 2 D PAGE
Komal Sharma
August 23, 2025Done ✅
Bhavana kankhedia
August 24, 2025in 2D-PAGE
Bharti Yadav
August 24, 20252D PAGE electrophoresis technique use the isoelectric focusing
Urmi sisodiya
August 24, 20252d page because it seperate the molecules on the basis of charge and size
Akshita Jangid
August 24, 20252D PAGE electrophoresis technique uses isoelectric focusing
Ankita Pareek
August 24, 2025Electrophoresis that use isoelectric focusing is 2D PAGE
Sonam
August 24, 20252D page
Alec
August 24, 2025option c – 2D PAGE is the correct ans.
Shivani Panwar
August 25, 20252d page
Niti Tanwar
August 24, 20252d page
Falguni Prakash
August 24, 20252D page
Kashish yadav
August 25, 20252D PAGE
sakshi khatri
August 25, 20252D page
Vijay kumar Meena
August 25, 20252 D page ✔️
Ayush Dubey
August 25, 20252D PAGE
Anju
August 25, 20252D PAGE
Surendra Doodi
August 27, 2025Isoelectric focusing (IEF) is a technique used in Two-Dimensional Gel Electrophoresis (2D-PAGE) as the first dimension to separate proteins based on their isoelectric point
Pratibha Jain
August 30, 2025Correct answer is option (C) 2D PAGE
Sheikh Aleen
August 30, 20252 D PAGE
Anisha Beniwal
August 30, 20252D PAGE
Neeraj Sharma
August 30, 2025In 2D PAGE first the sample is run in IEF electrophoresis hence option C
Vanshika Sharma
August 31, 20252 D PAGE
Shobha Kanwar
August 31, 2025Option(c) 2D gel electrophoresis techniques
Akshita Jangid
September 1, 20252D PAGE
Asha Gurzzar
September 1, 20252D pAGE will be the technique
Anisha jakhar
September 3, 20252D PAGE
Muskan Yadav
September 3, 20252D PAGE separate proteins based on their isoelectric point (pI)
Ajay Sharma
September 4, 2025It’s 2d page , one dimension seperate protein on the basis of charge (ph gradient) called isoelectric focusing and one seperate on the basis of molecular weight that is gel electrophoresis
Somya Yadav
September 5, 20252D PAGE
Aakansha sharma Sharma
October 1, 2025Correct answer: (C) 2D PAGE