Understanding Hybridization in DNA Fingerprinting
The work ‘Hybridization’ in DNA finger printing means
a. Pairing between the nucleotides of DNA sample with probe
b. Pairing between the nucleotides of DNA and mRNA
c. Pairing between the nucleotides of probe with mRNA
d. Pairing between the nucleosides with mRNA
What Does Hybridization Mean in DNA Fingerprinting?
DNA fingerprinting is a molecular technique used for identifying genetic differences among individuals. A key step in this process is hybridization, which refers to the pairing of complementary nucleotide sequences.
The correct answer is:
(A) Pairing between the nucleotides of the DNA sample with the probe
How Hybridization Works in DNA Fingerprinting
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DNA Extraction & Fragmentation
- DNA is first extracted from the sample (blood, saliva, hair, etc.).
- The DNA is then cut into fragments using restriction enzymes.
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Gel Electrophoresis
- The fragmented DNA is loaded onto an agarose gel and separated using an electric field.
- Smaller DNA fragments migrate faster, while larger ones move slower.
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Transfer to a Membrane (Southern Blotting)
- The DNA fragments are transferred onto a nylon or nitrocellulose membrane through capillary action.
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Hybridization with a DNA Probe
- A radioactive or fluorescent-labeled DNA probe is added to the membrane.
- This probe contains a specific DNA sequence that is complementary to the target DNA.
- The probe binds to matching sequences in the DNA sample through base-pairing (A-T, G-C).
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Detection
- The labeled probe allows the identification of specific DNA sequences using autoradiography or fluorescence scanning.
Why the Other Options Are Incorrect
-
(B) Pairing between the nucleotides of DNA and mRNA
- This describes transcription, not hybridization.
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(C) Pairing between the nucleotides of the probe with mRNA
- This occurs in Northern blotting, not in DNA fingerprinting.
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(D) Pairing between nucleosides with mRNA
- Nucleosides lack a phosphate group and do not participate in hybridization.
Conclusion
In DNA fingerprinting, hybridization refers to the binding of a DNA probe to its complementary DNA sequence in a sample. This specific base pairing allows scientists to identify genetic variations, making hybridization a crucial step in forensic investigations, paternity testing, and genetic research.
70 Comments
Ujjwal
March 8, 2025Okh sir
Mahima Sharma
August 21, 2025Binding of dna prob to be its complimentary dna sequences
Suman bhakar
March 8, 2025Done
Aafreen Khan
August 21, 2025Pairing between the nucleotides of the DNA sample with the probe becoz hybridization refers to the binding of a DNA probe to its complementary DNA sequence
Aakansha Sharma
August 20, 2025Option A
Aakansha Sharma
August 20, 2025Option A is right
Dipti Sharma
August 20, 2025A. Pairing between the nucleotides of the DNA sample with the probe.
Anurag Giri
August 20, 2025Ans a Pairing between the nucleotides of the DNA sample with the probe
hybridization, which refers to the pairing of complementary nucleotide sequences
Neelam Sharma
August 20, 2025Pairing between the nucleotides of the DNA sample with the probe
Nilofar Khan
August 20, 2025Pairing between the nucleotides of the dna sample and the probe
Dharmpal Swami
August 20, 2025Hybridization -binding dna probe to its completely dna sequence
Khushi Mehra
August 20, 2025In DNA fingerprinting, pairing is done between sample DNA and probe
Mansukh Kapoor
August 20, 2025The correct answer is option 1st
Hybridization refers to as the pairing of the DNA fragments with the probes
Priya dhakad
August 20, 2025Pairing between the nucleotides of the DNA sample with the probe answer first is correct
anjani sharma
August 20, 2025Answer 1 Pairing between the nucleotides of the DNA sample with the probe
Kavita choudhary
August 20, 2025Paring between the nucleotide of the DNA sample with the probe
Surbhi Rajawat
August 20, 2025Hybridization here refers to formation of a different or intermediate DNA different from original. Therefore it refers to the DNA binded with probe
Deepika Sheoran
August 20, 2025Pairing between the nucleotides of the DNA sample &the probe.
Neha
August 21, 2025Hybridization means in dna finger Printing pairing between nucleotide of the dna sample and the probe
Mohd juber Ali
August 21, 2025Option (a)
Priyanka choudhary
August 22, 2025Correct answer is option A
Pairing between the nucleotides of the DNA sample with the probe
Aman Choudhary
August 21, 2025Option A is correct
Pairing between the nucleotides of the DNA sample with the probe
Khushi Vaishnav
August 21, 2025Pairing between the nucleotides of the DNA sample with the probe
Sakshi yadav
August 21, 2025Pairing b/w the nucleotides of the DNA sample with the probe
Payal Gaur
August 21, 2025Pairing between the nucleotide of DNA wand probe include in DNA fingerprinting
Avni
August 21, 2025A) Pairing between the nucleotides of the DNA sample with the probe
Santosh Saini
August 21, 2025Pairing of complementary nucleotide sequence with probe
Divya rani
August 21, 2025Dna finger printing is the application of southern blotting so pairing between nucleotide of dna with probe is performed.
Simran Saini
August 21, 2025Pairing between the nucleotides of DNA sample with probe.
shruti sharma
August 22, 2025pairing between the nucleotide of dns sample with probe
Sakshi Kanwar
August 22, 2025As DNA fingerprinting means identifying specific DNA sequence using a DNA probe ……A
karishma
August 22, 2025Pairing between the nucleotides of the DNA sample with the probe
Sakshi
August 22, 2025A is correct option
Pair with nucleotideof dna sample with probe
Kirti agarwal
August 23, 2025Pairing between the nucleotide sequence of dna sample with probe
Priya khandal
August 23, 2025Pairing the between nucleotide with mrna
Akshita Jangid
August 23, 2025Pairing between the nucleotides of the DNA sample and the probe
Kajal
August 23, 2025Hybridization meaning in DNA finger printing is the pairing of nucleotides of dna sample with that of probe….
Bharti Yadav
August 23, 2025Hybridization in DNA fingerprinting means pairing between the nucleotides of the DNA sample with the probe
Yogita
August 24, 2025Pairing between the nucleotides of the DNA sample with the probe
Sneha Kumawat
August 24, 2025Pairing between the nucleotides of DNA sample with probe
Arushi Saini
August 24, 2025Pairing probe with DNA
Devika
August 24, 2025A. Is right
AKANKSHA RAJPUT
August 24, 2025hybridization means pairing of complementary nucleotide sequences.
Ankita Pareek
August 24, 2025Pairing of the nucleotide with probe is hybridization
Shivani
August 24, 2025The hybridization in dna fingerprinting means Pairing between the nucleotides with the probe
Bhavana kankhedia
August 24, 2025Pairing between the nucleotides of the DNA sample with the probe
Neeraj Sharma
August 24, 2025Option A is correct as DNA fingerprinting is nothing but southern blotting and in southern blotting the probe is get attached with specific DNA sample
Anisha Beniwal
August 24, 2025Option A
Ayush Dubey
August 24, 2025Pairing between the nucleotides of the DNA sample with the probe
Himanshi
August 24, 2025Pairing between the nucleotides of the DNA sample with the probe.
Tanvi Panwar
August 24, 2025Hybridization means pairing of nucleotides of DNA with DNA probes.
Surendra Doodi
August 24, 2025Option 1 is correct – Pairing between the nucleotides of the DNA sample with the probe
Priyanka Verma
August 24, 2025option A is correct because pairing between the nucleotides of DNA
Sonam Saini
August 24, 2025A is right answer
sakshi khatri
August 24, 2025a is correct
Falguni Prakash
August 24, 2025A option
Kashish yadav
August 25, 2025Option A
Kajal
August 25, 2025Pairing of nucleotide of dna with the probe
Priyanka Meena
August 26, 2025The work hybridization DNA finger printing bzc paring between the nucleotide of the DNA sample with the probe
Muskan Yadav
August 26, 2025Pairing between the nucleotides of the DNA sample with the probe.
Manisha choudhary
August 27, 2025Correct answer is Pairing of nucleotide with the DNA
Kanica Sunwalka
August 28, 2025done
Komal Sharma
August 29, 2025DNA fingerprinting is a molecular technique used for identifying genetic differences among individuals. A key step in this process is hybridization, which refers to the pairing of complementary nucleotide sequences.
Shriyanshi verma
August 30, 2025Binding of dna prob to be its complimentary dna sequences
Asha Gurzzar
August 30, 2025A is correct pairing between probe and dna nucleotide
Alec
August 30, 2025pairing between the nucleotides of the Dna sample with the probe.
Pratibha Jain
August 30, 2025The correct answer is option (A)
Pairing between the nucleotides of the DNA sample with the probe
Anisha jakhar
August 31, 2025A is the correct option
Shobha Kanwar
August 31, 2025The correct answer is:
(A) Pairing between the nucleotides of the DNA sample with the probe
Anjali
September 1, 2025Pairing between the nucleotides of the DNA sample with the probe