APS (Ammonium persulphate) is used in SDS-PAGE for
(A) preventing oxidation
(B) cross-linking
(C) its role as a catalyst
(D) free radical formation
Correct answer: (D) Free radical formation
Explanation:
Ammonium persulfate (APS) is a crucial component in SDS-PAGE because it helps in the polymerization of acrylamide to form the gel matrix.
- APS acts as an initiator by generating free radicals.
- When combined with TEMED (Tetramethylethylenediamine), APS decomposes into sulfate radicals, which initiate the polymerization of acrylamide and bisacrylamide to form the polyacrylamide gel.
Why Not the Other Options?
-
(A) Preventing oxidation ❌
- APS does not prevent oxidation; instead, it produces oxidative free radicals for polymerization.
-
(B) Cross-linking ❌
- Bisacrylamide (not APS) is responsible for cross-linking in SDS-PAGE.
-
(C) Its role as a catalyst ❌
- APS is not exactly a catalyst; it acts as a radical initiator.
- TEMED is the actual catalyst that accelerates APS decomposition to generate free radicals.
Final Answer:
✅ (D) Free radical formation
67 Comments
Prami Masih
February 27, 2025Well done sir
Mahima Sharma
August 16, 2025Free radical provider
Mahima Sharma
August 24, 2025Free radical formation
Arushi
April 1, 2025👍✔️
Anita choudhary
April 18, 2025✅✅
Anurag Giri
August 13, 2025Ans 4 free radical formation
Khushi Agarwal
August 13, 2025Aps is free radical provider
Soniya Shekhawat
August 13, 2025AMMONIUM PER SULPHATE provide free radical for polymerization of acrylamide.
Aman Choudhary
August 13, 2025option d is correct APS is a free radical provider
Deepika sheoran
August 14, 2025Aps is free Radical formation.
Mohd juber Ali
August 14, 2025Aps is a free radical provider for polymerization
Dharmpal Swami
August 14, 2025Free radical formation
Priya dhakad
August 14, 2025Option d is correct
Aps provide free radical.
Vijay kumar Meena
August 25, 2025Free radical formation
Neelam Sharma
August 14, 2025Free radical
Anjani sharma
August 14, 2025Answer 4 provide free radical
Aafreen
August 16, 2025For Free radical formation
Sneha Kumawat
August 14, 2025Answer is free radical formation
shruti sharma
August 14, 2025free radical formation
Avni
August 14, 2025Correct answer is (D) Free radical formation
Mansukh Kapoor
August 14, 2025The correct answer is option 4th
Aps provides free radicals
Priyanka choudhary
August 15, 2025Aps is free radial provided
Dipti Sharma
August 14, 2025Answer is (D) Free radical formation.
Nilofar Khan
August 14, 2025Answer is d
Karishma
August 14, 2025generate free radicals
Priti khandal
August 14, 2025Answer d is correct free radical formation
Priya khandal
August 14, 2025Free radical formation
Roopal Sharma
August 14, 2025Aps is a free radicle generator and initiate polymerization of acrylamide and bisacrylamide
Deepika Sheoran
August 15, 2025Option D is correct answer
Free radical formation.
Shivani
August 15, 2025APS forms free radical
Mitali saini
August 16, 2025Correct answer: (D) Free radical formation
Divya rani
August 16, 2025Yes Aps is a free radical provider and in SDS catalyst is TEMED
Mahima Sharma
August 16, 2025Free radical provider
Payal
August 17, 2025Aps used for free radical formation
Kavita choudhary
August 17, 2025Answer is d free radical formation
Kajal
August 17, 2025Free radical formation
Sakshi
August 17, 2025D is correct option
Because ammonium per sulphate releasesd free radical in sds
Varsha Tatla
August 19, 2025Aps is a important role plays in SDS PAGE it helps in polymerization of acrylamide by providing the free radical
Alec
August 24, 2025option d – it is used for free radical formation.
Manisha choudhary
August 20, 2025PAGE m polymerization free radical dwara hota h
Aps oxidation ko prevent nhi krta balki oxidative free radical provide krwata h jo polymerization k initiation k liye bhi important h
Catalyst TEMED hota h jo covalent bond formation krwata h
Bhavana kankhedia
August 24, 2025free radical formation.
Bharti Yadav
August 24, 2025Free radical formation
Priyanka Choudhary
August 24, 2025D is correct answer,Free radical formation
Sonam Saini
August 24, 2025Mukh mulk formation
Niti Tanwar
August 24, 2025Free radical formation
Anjali
August 24, 2025Aps used for free radical formation
Falguni Prakash
August 24, 2025Free radical
Sakshi
August 24, 2025Ammonium per sulphate is free radical formation
Devika
August 25, 2025Free radical formation
Kashish yadav
August 25, 2025Free radical formation
sakshi khatri
August 25, 2025free radical formation
sakshi khatri
August 25, 2025done
Ayush Dubey
August 25, 2025Free radical formation
Surendra Doodi
August 27, 2025Free radical formation
Monika meena
August 27, 2025Ammonium per sulphate (APS) provide free radicals formation
Monika meena
August 27, 2025Ammonium per sulphate (APS) provide free radicals formation ✅
Pratibha Jain
August 30, 2025Correct answer is option (D) Free radical formation
Sheikh Aleen
August 30, 2025Free radical formation
Anisha Beniwal
August 30, 2025Free radical formation
Vanshika Sharma
August 31, 2025Free radical formation
Shobha Kanwar
August 31, 2025D is correct answer,Free radical formation
Akshita Jangid
September 1, 2025APS is used in SDS PAGE for free radical formation
Asha Gurzzar
September 1, 2025D is correct,for providing free radicals
Anisha jakhar
September 3, 2025(D) Free radical formation
Muskan Yadav
September 3, 2025Free radical formation
Somya Yadav
September 5, 2025APS used as free radical provider
Aakansha sharma Sharma
October 1, 2025Correct answer: (D) Free radical formation