Which of these is an advantage of difference in gel electrophoresis (DiGE) compared to gel free approaches
(a) More sensitive
(b) Intact proteins allow detection of changes in protein modification
(c) Allows a greater range of proteins to be analyzed
(d) Proteins are identified as part of quantification
The correct answer is:
(b) Intact proteins allow detection of changes in protein modification
Explanation:
Difference in Gel Electrophoresis (DiGE) is a powerful 2D gel-based proteomics technique that allows the comparison of multiple protein samples on the same gel using different fluorescent dyes. Unlike gel-free approaches (such as mass spectrometry-based shotgun proteomics), DiGE retains proteins in their intact form, making it especially useful for detecting post-translational modifications (PTMs) like phosphorylation, glycosylation, and acetylation.
- (a) More sensitive → While DiGE is sensitive, gel-free approaches (e.g., LC-MS/MS) can sometimes offer even higher sensitivity.
- (c) Allows a greater range of proteins to be analyzed → Gel-free methods generally provide broader proteome coverage.
- (d) Proteins are identified as part of quantification → In DiGE, protein identification requires an additional mass spectrometry (MS) step, whereas gel-free approaches integrate identification and quantification.
Thus, (b) Intact proteins allow detection of changes in protein modification is the best answer. ✅
81 Comments
Lavanya
February 26, 2025Ok
Mahima Sharma
August 24, 2025Allow to be More quantity of protein to be analysed
Suman bhakar
February 26, 2025Ok
Ashwini Shekhawat
February 26, 2025Done sir
Parul
February 26, 2025Sir in explanation answer is b whereas in video explanations correct answer is C
Lokesh kumawat
February 26, 2025I have dout in this questions according to video explanation sir ne 2d-page se compare krke option c ans diya jo ki shi hai but 2d page bhi toh gel technique hi hai na pr yha question me pucha hai compare to gel free tech. Toh according toh written explanation option B statement is right but ye dono es question ke answer kaise ho skte hai if option B correct answer hota hai toh vo dige ka advantage kaise hua ye toh gel free tech ka explaination hai
Prami masih
February 26, 2025👍✅
Pramila kumari
February 26, 2025👍✅
Prami masih
February 26, 2025Okay 👍
Arushi saini
February 26, 2025👍👍
Pooja jat
February 26, 2025Done
Shivani meena
February 26, 2025Done ✅
Priya khandal
February 26, 2025Ok sir
Payal Soni
February 27, 2025Sir explanation me B answer de rkha h or video me sir ne C bataya h
Jyoti Meena
February 27, 2025Done
TAMANNA FOGAT
February 27, 2025sir whic h is correct ans b or c
Kabeer narwal
February 27, 2025Answer is C
Ujjwal
February 27, 2025Done 👍
Akshita
August 14, 2025Correct answer B
Intact proteins allow detection of changes in protein modification
Ujjwal
February 27, 2025Good
yogesh sharma
February 27, 2025Great explanation sir 🙏
yogesh sharma
February 27, 2025Sir option confusion
Rohit Meena
February 28, 2025Okay
Abhilasha
March 1, 2025✅
Meena Kumari
March 2, 2025Sir option confusion kyu h
Sumit Sultan
March 3, 2025Done ✔️
VIKRAM GAHLOT
March 5, 2025Nice sir
Anita choudhary
April 19, 2025Which is correct b or c
Khushi Agarwal
August 14, 2025Intact proteins allow detection of changes in protein modification
Dharmpal Swami
August 14, 2025Option B write
Neelam Sharma
August 14, 2025Allows a greater range of proteins to be analyzed
Savita Garwa
August 14, 2025B ryt answer
Deekshita Khandelwal
August 14, 2025Done
Mitali
August 14, 2025(b) Intact proteins allow detection of changes in protein modification is the best answer. ✅
Soniya Shekhawat
August 14, 2025I think c option is correct bcz by DIGE we analyse greater range of protein.
Meenakshi Choudhary
August 14, 2025I think c option is correct bcz dige allow a greater range of proteins to be analyzed
Sneha Kumawat
August 14, 2025The answer is allow a greater range of proteins to be analysed it is advantage of DIGE
Urmi
August 24, 2025I think option c is correct bcoz in dige we can analyse greater range of proteins with help of different flurochrome.
Arushi
August 14, 2025Option c should be correct
Dipti Sharma
August 14, 2025Answer is C
Deepika Sheoran
August 14, 2025Option b is correct
DIGE we analyse greater range of protein.
Monika jangid
August 14, 2025Intact protein allow detection of changes
Parul Yadav
August 14, 2025Compared to gel free approach DiGE retains proteins in their intact form hence useful in detecting PTM.
Priya khandal
August 14, 2025Intact proteins allow detection of change in protein modification
Ankita Pareek
August 15, 2025Intact protein allow detection of change in protein modification
shruti sharma
August 15, 2025option c
Priyanka Choudhary
August 24, 2025Correct answer is the Intact proteins allow detection of changes in protein modification
Shivani
August 15, 2025Option C is correct answer .
Kajal
August 15, 2025Intact protein allow detection of change in protein modification
Kavita choudhary
August 17, 2025Option b
Khushi Vaishnav
August 17, 2025Intact proteins allow detection of changes in protein modification
Neeraj Sharma
August 17, 2025Answer is c as the DiGE will helps us to analyze more proteins as we use different fluorochromes
Simran Saini
August 18, 2025Intact proteins allow detection of changes in protein modification.
Komal Sharma
August 19, 2025Done ✅
Bharti Yadav
August 24, 2025Intact proteins allow detection of changes in protein modification
karishma don
August 24, 2025DiGE protein ko intact form meh rkhta hai and also it detects the post translational modifications
Varsha Tatla
August 24, 2025Diff. In gel electrophoresis crucial role play in detection of protein modification
Priyanka Choudhary
August 24, 2025I think c is right answer,Allows a greater range of proteins to be analyzed
Priya dhakad
August 24, 2025Intact proteins allow detection of changes in protein modification.
Anil Choudhary
August 24, 2025Right answer= Intact proteins allow detection of changes in protein modification
Bhavana kankhedia
August 24, 2025Intact proteins allow detection of changes in protein modification
Sonam
August 24, 2025इंटेक्स प्रोटीन allow डिटेक्शन ऑफ़ चेंज इन प्रोटीन मोडिफिकेशन
Alec
August 24, 2025done sir
Anju
August 24, 2025Ans: b
Analysis of multiple protein sample can be done using different dyes
Falguni Prakash
August 24, 2025C
Kashish yadav
August 25, 2025Ok sir
Ayush Dubey
August 25, 2025Intact proteins allow detection of changes in protein modification
sakshi khatri
August 25, 2025done
Pratibha Jain
August 30, 2025The correct answer is option (b)
Intact proteins allow detection of changes in protein modification
Shriyanshi verma
August 30, 2025Allow to be More quantity of protein to be analysed
Sheikh Aleen
August 30, 2025Intact proteins allow detection of changes in protein modification
Surendra Doodi
August 30, 2025??
Patel Radha
August 30, 2025Done
anjani sharma
August 30, 2025Intact proteins allow detection of change in protein modification
Vanshika Sharma
August 31, 2025Intact protein allow detection of change in protien modification
Shobha Kanwar
August 31, 2025b) Intact proteins allow detection of changes in protein modification is the best answer
Ajay Sharma
September 2, 2025Option b is more correct in this regard
Anisha jakhar
September 3, 2025(b) Intact proteins allow detection of changes in protein modification
Muskan Yadav
September 3, 2025Intact proteins allow detection of changes in protein modification
Meera Gurjar
September 13, 2025Intact protein allows in detecting of change in protein
Aakansha sharma Sharma
October 1, 2025(b) Intact proteins allow detection of changes in protein modification