A mixture of Cytochrome-C (MW 11.7 kD) and Myoglobin (MW 17.2 KD) are to be separated by polyacrylamide gel electrophoresis. Their isoelectric pH (pI) values are 9.6 and 7.2 respectively. […]
Tag: Two-Dimensional Polyacrylamide Gel Electrophoresis
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CSIR NET Previous Year Questions Gel Electrophoresis
Determination of the molecular weight by molecular exclusion chromatography
A protein was purified to homogeneity. Determination of the molecular weight by molecular exclusion chromatography yields 60 kd. Chromatography in the presence of 6 M urea yields a 30-kd species. […]
CSIR NET Previous Year Questions Gel Electrophoresis
DNA replication with radio-labeled nucleotides, followed by urea-polyacrylamide gel electrophoresis (PAGE)
In an invitro experiment using radio-labeled nucleotides, a researcher is trying to analyze the possible products or DNA replication by resolving the products using urea-polyacrylamide gel electrophoresis. In one experimental […]
CSIR NET Previous Year Questions Gel Electrophoresis
To determine the Isoelectric point of a protein
To determine the isoelectric point of a protein, first establish that a gel: (a) Contains a denaturing detergent that can distribute uniform negative charges over the protein’s surface. (b) Exhibits […]
CSIR NET Previous Year Questions Gel Electrophoresis
Two-dimensional gel electrophoresis (2D-GE)
The first step in two-dimensional gel electrophoresis generates a series of protein bands by isoelectric focusing. In a second step a strip of this gel is turned 90 degrees, placed […]
CSIR NET Previous Year Questions Gel Electrophoresis
Determine the isoelectric point of a protein
To determine the isoelectric point of a protein, first establish that a gel: (a) Contains a denaturing detergent that can distribute uniform negative charges over the protein’s surface. (b) Exhibits […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS-PAGE & Protein Separation Role of SDS Explained
By adding SDS (sodium dodecyl sulfate) during the electrophoresis of proteins, it is possible to: (a) Determine a protein’s isoelectric point. (b) Determine an enzyme’s specific activity. (c) Determine the […]
CSIR NET Previous Year Questions Gel Electrophoresis
The electrophoretic mobility of migration of different biomolecules
The electrophoretic mobility of migration of different biomolecules In electrophoresis, the electrophoretic mobility determines the characteristics of migration of different biomolecules. Which of the following is not having any influence […]
CSIR NET Previous Year Questions Gel Electrophoresis
In SDS PAGE of protein one SDS molecule will bind to
In SDS PAGE of protein molecule separation, one SDS molecule will bind to (a) Every amino acid (b) Every two amino acids (c) Every three amino acids (d) Every four […]
CSIR NET Previous Year Questions Gel Electrophoresis
Advantage of discontinuous buffer system in SDS and native PAGE
The main advantage of discontinuous buffer system in SDS and native PAGE is (a) Conformation of protein is conserved (b) Constantly maintain the charge of proteins (c) Assist in migration […]
