Tag: Splicing

12. The 3’ end of most eukaryotic mRNAs is defined by the addition of a polyA tail- a processing reaction called polyadenylation. The addition of poly A tail is carried […]

11. You are working with an in vitro eukaryotic transcription system, which produced both capped and uncapped mRNAs. You incubated these mRNAs with mammalian cell nuclear extract and then quantified […]

Introns in Eukaryotic Genes: Found in rRNA, tRNA, and mRNA Encoding Genes 10. Introns in the eukaryotic genes are found in: (1) rRNA and mRNA encoding genes but not in […]

9. A gene consists of two introns, a 5′ UTR and 3’UTR region, the probable number of exon will be. (1) 2             (2) 3 (3) 4             (4) 5 Calculating the […]

8. An eukaryotic cell undergoing mRNA synthesis and processing was incubated with32P labelled ATP, with the label at the β-position. Where do you think the radioactive isotope will appear in […]

7. HeLa cell extract was used to study transcription of a gene ‘X’ having six introns. RNA POI II complex containing all associated proteins was isolated from actively transcribing system […]

Function of the Carboxyl-Terminal Domain (CTD) of RNA Polymerase II: Identifying the Non-Associated Role 6. The Carboxyl-Terminal domain (CTD) of RNA polymerase II consists of heptapeptide repeats (YSPTSPS). Other proteins […]

5. Eukaryotic mRNAs have an enzymatic appended cap structure consisting of a 7-methylguanosine residue joined to the initial 5′ nucleotide of the transcripts. Given below are a few statements regarding […]

4. Eukaryotic mRNA are modified to possess a 5′ cap structure. Which one of the following is an INCORRECT statement about the function of the 5′ cap structure? (1) It […]

3. Which of the following is necessary for transport of m-RNA from nucleus (1) Alternative Splicing (2) 5′-Capping (3) RNA editing (4) Secondary structure Necessary Factor for mRNA Transport from […]