Serum protein is separated in 1% agarose gel, 25minutes at 100 volts, stained with Ponseau S protein stain. The
separation of the protein is not optimal. What would you do to improve the separation
(a) Increase the agarose concentration
(b) Run the electrophoresis at 75V instead of 100
(c) Run the electrophoresis for 15 minutes instead of 25 minutes
(d) Use a lipid stain instead of protein stain
Correct answer: (A) Increase the agarose concentration
Explanation:
The resolution of protein separation in agarose gel electrophoresis depends on several factors, including gel concentration, voltage, and running time.
- Increasing the agarose concentration improves the pore size of the gel, allowing better separation of serum proteins based on charge and size.
- Serum proteins vary in molecular weight, and using a higher agarose concentration (e.g., 1.5% or 2% instead of 1%) provides better resolution, especially for smaller proteins.
Why Not the Other Options?
-
(B) Run the electrophoresis at 75V instead of 100V ❌
- Lower voltage slows down migration and may reduce resolution instead of improving it.
- Optimizing agarose concentration is more effective than lowering voltage.
-
(C) Run the electrophoresis for 15 minutes instead of 25 minutes ❌
- Shortening the run time may lead to insufficient separation, causing poor resolution.
- Instead, running for a longer time at optimal voltage could enhance separation.
-
(D) Use a lipid stain instead of a protein stain ❌
- Ponseau S is a protein stain, which is appropriate for serum protein analysis.
- A lipid stain (e.g., Sudan Black) is not relevant for protein separation.
Final Answer:
✅ (A) Increase the agarose concentration
68 Comments
Nisha
February 25, 2025Ok
Parul
February 25, 2025Easily understood. Okay done
Mitali
August 15, 2025(A) Increase the agarose concentration
Ritika Jangir
February 26, 2025Done
Shreeji Charam
February 26, 2025Guessed it wright but reason explanation se samaj aaya
Lavanya Sharma
February 26, 2025Explained well!
Lavanya Sharma
February 26, 2025Explained nicely.
Suman bhakar
February 26, 2025Done
Ujjwal
February 26, 2025Done
Ujjwal
February 26, 2025Okh done
Ashwini Shekhawat
February 26, 2025Done
Arushi
February 26, 2025☑️
Pooja jat
February 27, 2025Done.
Kabeer Narwal
February 27, 2025👍
Prami Masih
February 27, 2025Very nice sir
BANWARI lal khatana
February 27, 2025Well done ✅
yogesh sharma
February 27, 2025Well defined sir ☺️
Lokesh kumawat
February 27, 2025Explanation is perfect done
yogesh sharma
February 27, 2025The way sir explain topics are easiest
Abhilasha
March 1, 2025✅
Beena Meena
March 2, 2025✅
Priya khandal
March 2, 2025Done sir
VIKRAM GAHLOT
March 7, 2025👍
Anita choudhary
April 19, 2025✅
Mohd juber Ali
August 14, 2025agarose concentration increase karke then the separation of protein is optimal
Khushi Agarwal
August 14, 2025Option a is correct
Bcs conc increase krenge to pore size decrease hogi to protein clr dekh lenge
Anjani sharma
August 14, 2025Increase the concentration of agarose will improve pore size ,so that protein can be detected clearly
Soniya Shekhawat
August 14, 2025By increasing the concentration of agrose the pose size is decrease so protein is easily detected.
Sneha Kumawat
August 14, 2025The correct answer is increase the concentration of agrose gel
Karishma Don
August 14, 2025the resolution of protein separation depends on gel conc. , voltage and running time. So the inc. conc. of agarose will lead to small pores and high resolution of proteins
Dipti Sharma
August 14, 2025(A) Increase the agarose concentration will improve pore size
Roopal Sharma
August 14, 2025By increasing tha afrose concentration so pore size is increased
Monika jangid
August 14, 2025Inc. Agarose gel conc bcoz it improve pore size and protein detected
Priti khandal
August 14, 2025Increase Agarose concertration A option is right
Priya khandal
August 14, 2025Increase the agarose concentration
Priyanka choudhary in
August 15, 2025Right answer is increase the agarose concentration
Shivani
August 15, 2025Increase the agarose concentration
Divya rani
August 16, 2025Serum protein’s molecular weight is more so when we increase the concentration of Agarose Then the pore will produce in small size Then the separation of serum protein will better observed .
shruti sharma
August 16, 2025a is correct ans
Aafreen
August 17, 2025Increase the agarose concentration
Kajal
August 17, 2025Option a
Inc. the agarose concentration
Avni
August 17, 2025Increase the agarose concentration
Simran Saini
August 18, 2025Increase the agarose concentration.
Meenakshi Choudhary
August 18, 2025A is correct answer
Neeraj Sharma
August 18, 2025Option A is correct as all the other three options are not suitable and also increasing the concentration of agarose will decrease the pore size which helps in better resolution
Komal Sharma
August 22, 2025The resolution of protein separation in agarose gel electrophoresis depends on several factors, including gel concentration, voltage, and running time so option A is correct
Bharti Yadav
August 24, 2025Increase the agarose concentration
Priyanka Choudhary
August 24, 2025Right answer is increase the agarose concentration
Urmi sisodiya
August 24, 2025Increase agarose concentration because the pore size get decreased and the molecules get seperate easily
Bhavana kankhedia
August 24, 2025Increase the agarose concentration
Sonam Saini
August 24, 2025Agarose Increase concentration
Anjali
August 24, 2025Increase agarose concentration .
Kashish yadav
August 25, 2025Increase the agarose concentration
sakshi khatri
August 25, 2025done
Ayush Dubey
August 25, 2025Increase the agarose concentration
Pratibha Jain
August 30, 2025Correct answer is option (A)
Increase the agarose concentration
Shriyanshi verma
August 30, 2025The correct answer is increase the concentration of agrose gel
Sheikh Aleen
August 30, 2025Increase the agarose concentration
Patel Radha
August 30, 2025Done
Anisha Beniwal
August 31, 2025Increase the agarose concentration
Shobha Kanwar
August 31, 2025Right answer is increase the agarose concentration
Vijay Marmat
September 5, 2025Increase the agarose concentration ✅️
Anisha jakhar
September 3, 2025(A) Increase the agarose concentration
Muskan Yadav
September 3, 2025Increase the agarose concentration
Ajay Sharma
September 4, 2025Should increase agarose concentration it will help increase pore size and channels further helps in resolving
Somya Yadav
September 5, 2025Inc. Agarose gel concentration
Meera Gurjar
September 13, 2025Increase agarose concentration
Aakansha sharma Sharma
October 1, 2025Correct answer: (A) Increase the agarose concentration