Which of the following statement about isoelectric focusing is correct
(a) Proteins separated by isoelectric focusing cannot be tested for biological activity
(b) Proteins separated by it can be tested for biological activity
(c) The separation of proteins is only based on charge
(d) The separation of protein is only based on size
✅ Correct answer: (C) The separation of proteins is only based on charge
Explanation:
Isoelectric Focusing (IEF) is a technique that separates proteins based on their isoelectric point (pI), which is the pH at which a protein has no net charge and stops moving in an electric field.
- Proteins migrate in a pH gradient until they reach their pI, where they have zero net charge and stop.
- The separation is entirely based on charge, not size.
- IEF is used in 2D-PAGE as the first dimension to separate proteins by charge before SDS-PAGE separates them by size.
Why Not the Other Options?
-
(A) Proteins separated by isoelectric focusing cannot be tested for biological activity ❌
- Incorrect, because IEF does not denature proteins as SDS-PAGE does.
- If mild conditions are used, proteins can retain biological activity.
-
(B) Proteins separated by it can be tested for biological activity ❌
- Partially true, but not always.
- If denaturing conditions (e.g., urea) are used, proteins may lose activity.
- However, native IEF can preserve function, making this statement not universally correct.
-
(D) The separation of protein is only based on size ❌
- Incorrect, because IEF does not separate proteins by size, only by charge (pI).
- SDS-PAGE separates by size, not IEF.
Final Answer:
✅ (C) The separation of proteins is only based on charge
58 Comments
Prami Masih
February 27, 2025Done 👍 sir
Mahima Sharma
August 24, 2025Seperate of protein based on chrge
Arushi
April 1, 2025👍✔️
Mohd juber Ali
August 14, 2025IEF separate protein on the basis of charge (PI)
Anurag Giri
August 14, 2025Ans c IEF is used in 2D-PAGE as the first dimension to separate proteins by charge
Khushi Agarwal
August 14, 2025Option c is correct
Saparates by charge
Aman Choudhary
August 14, 2025option C is correct it separate the protein on the basis of charge
Dharmpal Swami
August 14, 2025IEF- separate protein based on charge
Priya dhakad
August 14, 2025The separation of protein is based on charge so option c is correct
Anjani sharma
August 14, 2025Answer c charge
Sneha Kumawat
August 14, 2025The separation of protein is based on charge
Soniya Shekhawat
August 14, 2025Easy sir👍🏻
Mansukh Kapoor
August 14, 2025The correct answer is option 3rd
In IEF the separation is only on the basis of the charge
Nilofar Khan
August 14, 2025Correct answer is c
Nilofar Khan
August 14, 2025Correct answer is c
Karishma Don
August 14, 2025sepparation is based on charge
Dipti Sharma
August 14, 2025(C) The separation of proteins is only based on charge
Priyanka choudhary
August 15, 2025Correct answer is option c,the separation of protein is only based charge
Anju
August 25, 2025Ans:c
Pi=ph
So the separation is based on charge
Neelam Sharma
August 14, 2025Proteins separated by it can be tested for biological activity
Deepika Sheoran
August 14, 2025Option c is correct answer.
Saparation of protein is based on charge.
Priti khandal
August 14, 2025The separation of protein is only based upon charge
Komal Pareek
August 14, 2025a is correct
Roopal Sharma
August 14, 2025The separation is entirely bsses on charge
Mitali
August 15, 2025(C) The separation of proteins is only based on charge
Shivani
August 15, 2025(c). Separation of protein only based upon charge
Divya rani
August 16, 2025Yes right answer is C because IEF is only based on charge in a ampholyte ph gradient
Aafreen
August 17, 2025The separation of proteins is only based on charge
Payal Gaur
August 17, 2025Sepration of protein on the basis of charge in IEF
Kavita choudhary
August 17, 2025The separation of protein on base apon size
Kajal
August 17, 2025Isoelectric focusing is based on charge
Avni
August 17, 2025separation of protein is based on charge
Neeraj Sharma
August 18, 2025Option C is correct as IEF only separates the proteins on the basis of their charge and hence we get the pi of the proteins
Manisha choudhary
August 20, 2025Isoelectric focusing pi value k base p seprate krta h protein ko
Is s protein ki property pta nhi chlti saari too biological activity nhi pta kr skte is s
Protein ki pi value pta chlti h jis p protein ki perticular ph p net charge zero hota h
Bhavana kankhedia
August 24, 2025protein separation is based on charge
Bharti Yadav
August 24, 2025The separation of proteins is only based on charge
Priyanka Choudhary
August 24, 2025The separation of proteins is only based on charge ,c is correct answer
Bhavana kankhedia
August 24, 2025Isoelectric focusing is based on charge
Sonam Saini
August 24, 2025Separation of proteins is based only on charge
Alec
August 24, 2025option c – separation of proteins is based only on charge.
Niti Tanwar
August 24, 2025Option c
Separation of protein based only on charge
Sakshi
August 25, 2025Seperation of protein based on charge
Falguni Prakash
August 24, 2025Option c on the basis of charge
Kashish yadav
August 25, 2025Option c
sakshi khatri
August 25, 2025done
Vijay kumar Meena
August 25, 2025C ✔️
Ayush Dubey
August 25, 2025The separation of proteins is only based on charge
Devika
August 25, 2025The separation of protein is based on charge
shruti sharma
August 25, 2025The correct answer is option 3rd
In IEF the separation is only on the basis of the charge
Pratibha Jain
August 30, 2025Correct answer is option (C) The separation of proteins is only based on charge
Sheikh Aleen
August 30, 2025The separation of proteins is only based on charge
Anisha Beniwal
August 31, 2025The separation of proteins is only based on charge
Shobha Kanwar
August 31, 2025The separation of protein is based on charge so option c is correct
Asha Gurzzar
September 1, 2025Seperation of protein is only based on charge in isoelectric focusing,so option c is correct
Anisha jakhar
September 3, 2025The separation of proteins is only based on charge
Muskan Yadav
September 3, 2025The separation of proteins is only based on charge.
Somya Yadav
September 5, 2025IEF separates protein on the basis of charge
Aakansha sharma Sharma
October 1, 2025Correct answer: (C) The separation of proteins is only based on charge