In electrophoresis, protein will move until
(a) Its ph is greater than pi
(b) Its ph is smaller than pi
(c) Its ph is equal to pi
(d) Pi is greater than ph
The correct answer is:
(c) Its pH is equal to pI
Explanation:
In electrophoresis, proteins migrate based on their net charge, which depends on the surrounding pH relative to their isoelectric point (pI):
- When pH > pI → The protein carries a negative charge and moves towards the positive electrode (anode).
- When pH < pI → The protein carries a positive charge and moves towards the negative electrode (cathode).
- When pH = pI → The protein has no net charge (neutral) and stops migrating in the gel.
This is the principle behind Isoelectric Focusing (IEF), where proteins move until they reach their pI, at which point they stop migrating.
Thus, option (c) “Its pH is equal to pI” is correct. ✅
96 Comments
Lavanya Sharma
February 26, 2025Got it 👍🏻
Suman bhakar
February 26, 2025The best 👌👌
Arvind
February 26, 2025👍👍👍
Ujjwal
February 26, 2025Very nice sir
Ashwini Shekhawat
February 26, 2025💯
Parul
February 26, 2025Done sir. Understood
Abhilasha
February 26, 2025👍🏻
Arushi
February 26, 2025☑️
Abhilasha
February 26, 2025Done ✅
Lokesh kumawat
February 26, 2025Done
Vikram
February 26, 2025✅
Payal
February 27, 2025Yes sir
Pooja jat
February 27, 2025Got it 👍
Kabeer Narwal
February 27, 2025👍
Prami Masih
February 27, 2025Super sir
Priya khandal
February 27, 2025Ok sir
yogesh sharma
February 27, 2025Easy 😄
Nisha
February 27, 2025Understood
Pallavi
February 27, 2025Done
yogesh sharma
February 27, 2025Easy
Beena Meena
March 2, 2025✅
Beena Meena
March 2, 2025Done
Priti khandal
March 2, 2025Done Sir
Sumit Sultan
March 3, 2025✔️👍Done Sir
VIKRAM GAHLOT
March 7, 2025👍
Anita choudhary
April 19, 2025✅
Aman Choudhary
August 13, 2025option c is correct when ph = pi the net charge is neutral so it stop moving
Mahima Sharma
August 24, 2025Ph is 0 so stop moving
Anurag Giri
August 14, 2025Ans c In electrophoresis, proteins migrate based on their net charge, which depends on the surrounding pH relative to their isoelectric point When pH = pI The protein has no net charge (neutral) and stops migrating in the gel.
Khushi Agarwal
August 14, 2025Option 3 is correct
Pi = ph
Dharmpal Swami
August 14, 2025PH is equal to pi
Anjani sharma
August 14, 2025Option 3 when PH =PI
Neelam Sharma
August 14, 2025Its ph is equal to pi
Karishma Don
August 14, 2025ph=pI
Khushi Mehra
August 14, 2025Answer 3
Until pH =pI
Mitali
August 14, 2025option (c) “Its pH is equal to pI” is correct. ✅
Soniya Shekhawat
August 14, 2025ph is equal to pI …..
Roopal Sharma
August 14, 2025pH equal to pi
Priya dhakad
August 14, 2025PH is equal to PI becoz protein has no net charge and stop migrating in gel
Priya dhakad
August 14, 2025PH is equal to PI becoz protein has no net charge and stop migrating inthe gel
Mohd juber Ali
August 14, 2025Ans is (c)The protein move until they reach their PI
Means pH= PI ( neutral)
Mansukh Kapoor
August 14, 2025The correct answer is option 3rd
PI equals to the pH than protein stops migrating
Dipti Sharma
August 14, 2025(c) Its pH is equal to pI
Deepika Sheoran
August 14, 2025Ph is equal to PI. is correct.
HIMANI FAUJDAR
August 14, 2025Ans 3 pH =pI because net charge is zero
Monika jangid
August 14, 2025Ph= pi than stop migration
Surbhi Rajawat
August 14, 2025When Ph becomes equal to PI the protein becomes neutral and hence doesn not move towards the charged electrode
Priyanka choudhary
August 14, 2025pH and Pi level is equal
Priya khandal
August 14, 2025It’s pH equal to Pi
shruti sharma
August 15, 2025ph=pi option c
Shivani
August 15, 2025(c) in electrophoresis protein will move until it’s ph is equal to pI
Kajal
August 15, 2025Where pH equals to PI
Divya rani
August 16, 2025Ph=pi because net charge become zero so protein will not move.
Kavita choudhary
August 17, 2025pH is equal to pi
Aafreen
August 17, 2025Ans-3 Its ph is equal to pi
Khushi Vaishnav
August 17, 2025Ph is equal to pi
Avni
August 17, 2025(C) pH=pi
Neeraj Sharma
August 17, 2025Option C is correct as when the ph is get equal to the pi the net charge on the protein is zero so it will not further move as the separation is based on charge
Simran Saini
August 18, 2025ph is equal to pi.
Meenakshi Choudhary
August 18, 2025Its pH is equal to pI
Komal Sharma
August 22, 2025This is the principle behind Isoelectric Focusing (IEF), where proteins move until they reach their pI, at which point they stop migrating. So option 3 is correct bcz ph is equal to pi
Varsha Tatla
August 23, 2025pH equal to pl
Bharti Yadav
August 24, 2025When pH = pI The protein has no net charge (neutral) and stops migrating in the gel.
Priyanka Choudhary
August 24, 2025Option c is correct answer,Its pH is equal to pI
Ankita Pareek
August 24, 2025In electrophoresis protein will move until their ph is equal to pi
MOHIT AKHAND
August 24, 2025Option (C)
Done sir ✅
Urmi sisodiya
August 24, 2025When ph gets equal to pi then the migration becomes stop
Akshita Jangid
August 24, 2025In electrophoresis protein move until its ph is equal to pi
Anil Choudhary
August 24, 2025Right answer= C
Bhavana kankhedia
August 24, 2025Its pH is equal to pi
Sonam Saini
August 24, 2025its ph is equal to pi
Alec
August 24, 2025(c) protein will move until it’s ph = pi.
Niti Tanwar
August 24, 2025Protein will move pH =pi
C is right answer
Anjali
August 24, 2025option (c) “Its pH is equal to pI” is correct.
Seema
August 24, 2025C) protein will move until ph is equal to pi.
Anju
August 24, 2025Ans: c
Pi =ph
Seema
August 24, 2025Protein will move until ph is equal to pi.
Devika
August 24, 2025pH is equal to pi
Falguni Prakash
August 24, 2025C
Kashish yadav
August 25, 2025Ok sir
Ayush Dubey
August 25, 2025Its pH is equal to pI
sakshi khatri
August 25, 2025done
Pratibha Jain
August 30, 2025The correct answer is option (c)
Its pH is equal to pI
Shriyanshi verma
August 30, 2025option c is correct when ph = pi the net charge is neutral so it stop moving
Sheikh Aleen
August 30, 2025pH is equal to pI
Surendra Doodi
August 30, 2025In electrophoresis, proteins migrate based on their net charge, which depends on the surrounding pH relative to their isoelectric point .
When pH is equal to pi then protein have no any charge means neutral……so no moment in gel…
Patel Radha
August 30, 2025Option 3 is correct Pi = ph
Vanshika Sharma
August 31, 2025When protein has no net charge hence stops migrating
Shobha Kanwar
August 31, 2025Option 3 is correct As ph is equal to pi
Aartii sharma
September 1, 2025When ph is equal to pi
Anisha
September 3, 2025pH is equal to pi
Muskan Yadav
September 3, 2025Its pH is equal to pI
Ajay Sharma
September 4, 2025If both ph and pi are equal protein becomes neutral and stops moving
Somya Yadav
September 5, 2025Ph is equal to pi
Meera Gurjar
September 13, 2025pH is equal to P
Aakansha sharma Sharma
October 1, 2025correct answer is:
(c) Its pH is equal to pI