In electrophoresis, protein will move until

In electrophoresis, protein will move until

(a) Its ph is greater than pi
(b) Its ph is smaller than pi
(c) Its ph is equal to pi
(d) Pi is greater than ph

 

 

The correct answer is:

(c) Its pH is equal to pI

Explanation:

In electrophoresis, proteins migrate based on their net charge, which depends on the surrounding pH relative to their isoelectric point (pI):

  • When pH > pI → The protein carries a negative charge and moves towards the positive electrode (anode).
  • When pH < pI → The protein carries a positive charge and moves towards the negative electrode (cathode).
  • When pH = pI → The protein has no net charge (neutral) and stops migrating in the gel.

This is the principle behind Isoelectric Focusing (IEF), where proteins move until they reach their pI, at which point they stop migrating.

Thus, option (c) “Its pH is equal to pI” is correct. ✅

96 Comments
  • Lavanya Sharma
    February 26, 2025

    Got it 👍🏻

  • Suman bhakar
    February 26, 2025

    The best 👌👌

  • Arvind
    February 26, 2025

    👍👍👍

  • Ujjwal
    February 26, 2025

    Very nice sir

  • Ashwini Shekhawat
    February 26, 2025

    💯

  • Parul
    February 26, 2025

    Done sir. Understood

  • Abhilasha
    February 26, 2025

    👍🏻

  • Arushi
    February 26, 2025

    ☑️

  • Abhilasha
    February 26, 2025

    Done ✅

  • Lokesh kumawat
    February 26, 2025

    Done

  • Vikram
    February 26, 2025

  • Payal
    February 27, 2025

    Yes sir

  • Pooja jat
    February 27, 2025

    Got it 👍

  • Kabeer Narwal
    February 27, 2025

    👍

  • Prami Masih
    February 27, 2025

    Super sir

  • Priya khandal
    February 27, 2025

    Ok sir

  • yogesh sharma
    February 27, 2025

    Easy 😄

  • Nisha
    February 27, 2025

    Understood

  • Pallavi
    February 27, 2025

    Done

  • yogesh sharma
    February 27, 2025

    Easy

  • Beena Meena
    March 2, 2025

  • Beena Meena
    March 2, 2025

    Done

  • Priti khandal
    March 2, 2025

    Done Sir

  • Sumit Sultan
    March 3, 2025

    ✔️👍Done Sir

  • VIKRAM GAHLOT
    March 7, 2025

    👍

  • Anita choudhary
    April 19, 2025

  • Aman Choudhary
    August 13, 2025

    option c is correct when ph = pi the net charge is neutral so it stop moving

    • Mahima Sharma
      August 24, 2025

      Ph is 0 so stop moving

  • Anurag Giri
    August 14, 2025

    Ans c In electrophoresis, proteins migrate based on their net charge, which depends on the surrounding pH relative to their isoelectric point When pH = pI The protein has no net charge (neutral) and stops migrating in the gel.

  • Khushi Agarwal
    August 14, 2025

    Option 3 is correct
    Pi = ph

  • Dharmpal Swami
    August 14, 2025

    PH is equal to pi

  • Anjani sharma
    August 14, 2025

    Option 3 when PH =PI

  • Neelam Sharma
    August 14, 2025

    Its ph is equal to pi

  • Karishma Don
    August 14, 2025

    ph=pI

  • Khushi Mehra
    August 14, 2025

    Answer 3
    Until pH =pI

  • Mitali
    August 14, 2025

    option (c) “Its pH is equal to pI” is correct. ✅

  • Soniya Shekhawat
    August 14, 2025

    ph is equal to pI …..

  • Roopal Sharma
    August 14, 2025

    pH equal to pi

  • Priya dhakad
    August 14, 2025

    PH is equal to PI becoz protein has no net charge and stop migrating in gel

  • Priya dhakad
    August 14, 2025

    PH is equal to PI becoz protein has no net charge and stop migrating inthe gel

  • Mohd juber Ali
    August 14, 2025

    Ans is (c)The protein move until they reach their PI
    Means pH= PI ( neutral)

  • Mansukh Kapoor
    August 14, 2025

    The correct answer is option 3rd
    PI equals to the pH than protein stops migrating

  • Dipti Sharma
    August 14, 2025

    (c) Its pH is equal to pI

  • Deepika Sheoran
    August 14, 2025

    Ph is equal to PI. is correct.

  • HIMANI FAUJDAR
    August 14, 2025

    Ans 3 pH =pI because net charge is zero

  • Monika jangid
    August 14, 2025

    Ph= pi than stop migration

  • Surbhi Rajawat
    August 14, 2025

    When Ph becomes equal to PI the protein becomes neutral and hence doesn not move towards the charged electrode

  • Priyanka choudhary
    August 14, 2025

    pH and Pi level is equal

  • Priya khandal
    August 14, 2025

    It’s pH equal to Pi

  • shruti sharma
    August 15, 2025

    ph=pi option c

  • Shivani
    August 15, 2025

    (c) in electrophoresis protein will move until it’s ph is equal to pI

  • Kajal
    August 15, 2025

    Where pH equals to PI

  • Divya rani
    August 16, 2025

    Ph=pi because net charge become zero so protein will not move.

  • Kavita choudhary
    August 17, 2025

    pH is equal to pi

  • Aafreen
    August 17, 2025

    Ans-3 Its ph is equal to pi

  • Khushi Vaishnav
    August 17, 2025

    Ph is equal to pi

  • Avni
    August 17, 2025

    (C) pH=pi

  • Neeraj Sharma
    August 17, 2025

    Option C is correct as when the ph is get equal to the pi the net charge on the protein is zero so it will not further move as the separation is based on charge

  • Simran Saini
    August 18, 2025

    ph is equal to pi.

  • Meenakshi Choudhary
    August 18, 2025

    Its pH is equal to pI

  • Komal Sharma
    August 22, 2025

    This is the principle behind Isoelectric Focusing (IEF), where proteins move until they reach their pI, at which point they stop migrating. So option 3 is correct bcz ph is equal to pi

  • Varsha Tatla
    August 23, 2025

    pH equal to pl

  • Bharti Yadav
    August 24, 2025

    When pH = pI The protein has no net charge (neutral) and stops migrating in the gel.

  • Priyanka Choudhary
    August 24, 2025

    Option c is correct answer,Its pH is equal to pI

  • Ankita Pareek
    August 24, 2025

    In electrophoresis protein will move until their ph is equal to pi

  • MOHIT AKHAND
    August 24, 2025

    Option (C)
    Done sir ✅

  • Urmi sisodiya
    August 24, 2025

    When ph gets equal to pi then the migration becomes stop

  • Akshita Jangid
    August 24, 2025

    In electrophoresis protein move until its ph is equal to pi

  • Anil Choudhary
    August 24, 2025

    Right answer= C

  • Bhavana kankhedia
    August 24, 2025

    Its pH is equal to pi

  • Sonam Saini
    August 24, 2025

    its ph is equal to pi

  • Alec
    August 24, 2025

    (c) protein will move until it’s ph = pi.

  • Niti Tanwar
    August 24, 2025

    Protein will move pH =pi
    C is right answer

  • Anjali
    August 24, 2025

    option (c) “Its pH is equal to pI” is correct.

  • Seema
    August 24, 2025

    C) protein will move until ph is equal to pi.

  • Anju
    August 24, 2025

    Ans: c
    Pi =ph

  • Seema
    August 24, 2025

    Protein will move until ph is equal to pi.

  • Devika
    August 24, 2025

    pH is equal to pi

  • Falguni Prakash
    August 24, 2025

    C

  • Kashish yadav
    August 25, 2025

    Ok sir

  • Ayush Dubey
    August 25, 2025

    Its pH is equal to pI

  • sakshi khatri
    August 25, 2025

    done

  • Pratibha Jain
    August 30, 2025

    The correct answer is option (c)
    Its pH is equal to pI

  • Shriyanshi verma
    August 30, 2025

    option c is correct when ph = pi the net charge is neutral so it stop moving

  • Sheikh Aleen
    August 30, 2025

    pH is equal to pI

  • Surendra Doodi
    August 30, 2025

    In electrophoresis, proteins migrate based on their net charge, which depends on the surrounding pH relative to their isoelectric point .
    When pH is equal to pi then protein have no any charge means neutral……so no moment in gel…

  • Patel Radha
    August 30, 2025

    Option 3 is correct Pi = ph

  • Vanshika Sharma
    August 31, 2025

    When protein has no net charge hence stops migrating

  • Shobha Kanwar
    August 31, 2025

    Option 3 is correct As ph is equal to pi

  • Aartii sharma
    September 1, 2025

    When ph is equal to pi

  • Anisha
    September 3, 2025

    pH is equal to pi

  • Muskan Yadav
    September 3, 2025

    Its pH is equal to pI

  • Ajay Sharma
    September 4, 2025

    If both ph and pi are equal protein becomes neutral and stops moving

  • Somya Yadav
    September 5, 2025

    Ph is equal to pi

  • Meera Gurjar
    September 13, 2025

    pH is equal to P

  • Aakansha sharma Sharma
    October 1, 2025

    correct answer is:

    (c) Its pH is equal to pI

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