Gel Electrophoresis to work Negatively charged nucleic acids Agarose or polyacrylamide to separate Ethidium bromide (EtBr)

Which of the following is important for gel electrophoresis to work?
a. Negatively charged nucleic acids to migrate through the gel.
b. Ethidium bromide to provide a means to visualize the DNA in the gel.
c. Agarose or polyacrylamide to separate the DNA based on size.
d. All of the above are important for gel electrophoresis

 

The correct answer is:

(d) All of the above are important for gel electrophoresis.

Explanation:

Gel electrophoresis works by separating nucleic acids (DNA/RNA) or proteins based on size and charge. Each option listed is crucial for the process:

  1. (a) Negatively charged nucleic acids to migrate through the gel

    • DNA and RNA are negatively charged due to their phosphate backbone.
    • Under an electric field, they migrate toward the positive electrode (anode).
  2. (b) Ethidium bromide (EtBr) to provide a means to visualize the DNA in the gel

    • EtBr is a fluorescent dye that intercalates between DNA bases.
    • Under UV light, it makes DNA bands visible.
    • Alternative dyes like SYBR Safe or GelRed can also be used.
  3. (c) Agarose or polyacrylamide to separate the DNA based on size

    • Agarose gel is used for larger DNA fragments (100 bp to several kb).
    • Polyacrylamide gel is used for smaller DNA or RNA fragments and provides higher resolution.
    • The gel matrix acts like a sieve, allowing smaller fragments to migrate faster than larger ones.

Conclusion:

Since all three factors are essential for gel electrophoresis to function correctly, the correct answer is:

(d) All of the above are important for gel electrophoresis.

97 Comments
  • Parul
    February 25, 2025

    Okay sir able to do it and understood the importance and difference between knowledge available in books and provided by you in classes.

  • Alina mansuri
    February 25, 2025

    Got it

    • Mahima Sharma
      August 24, 2025

      Negative charg nucleic acid
      Etbr to provide a visualization dna in the gel
      Agrose and page based on the size

  • Ujjwal
    February 25, 2025

    Good sir

  • Ritika Jangir
    February 26, 2025

    Done

  • Shreeji Charan
    February 26, 2025

    Was correct

  • Lavanya Sharma
    February 26, 2025

    Was correct sir

  • Suman bhakar
    February 26, 2025

    Done

  • Ashwini Shekhawat
    February 26, 2025

    To the point explanation ✨

  • Lokesh kumawat
    February 26, 2025

    Done

  • Abhilasha
    February 26, 2025

    Done

  • Arushi
    February 26, 2025

    ☑️👍

  • Shivani meena
    February 26, 2025

    Done ✅

  • Vikram
    February 26, 2025

    👍🏼

  • Vikram
    February 26, 2025

    👍🏼👍🏻

  • Vikram
    February 26, 2025

    🆗

  • Jyoti Meena
    February 27, 2025

    Done

  • Pooja jat
    February 27, 2025

    Nice to see point to point nd easy way of explanation.

  • Kabeer Narwal
    February 27, 2025

    👍👍

  • Prami Masih
    February 27, 2025

    Okay sir

  • yogesh sharma
    February 27, 2025

    Great explanation sir 🙏

  • Nisha
    February 27, 2025

    Nice way explantion

  • yogesh sharma
    February 27, 2025

    Got it sir

  • Priya khandal
    March 2, 2025

    Done sir

  • Sumit Sultan
    March 3, 2025

    👍👍💫

  • Anita choudhary
    April 18, 2025

    Done 👍✅

  • Anurag Giri
    August 13, 2025

    Ans is 4 Negatively charged nucleic acids to migrate through the gel towards positive electrode and Ethidium bromide (EtBr) to provide a means to visualize the DNA in the gel under uv light and agarose gel used for separation of larger dna frag. And polysacc. Use for small dna frag

  • Khushi Agarwal
    August 13, 2025

    Option 4 is correct here
    Tino point milke hi gel electrophoresis kaam karta hai
    Charge movement ke liye,
    Gel separation ke liye,
    Dye visualization ke liye.

  • Aman Choudhary
    August 13, 2025

    option d is correct All of the above are important for gel electrophoresis
    for movement charge is used, for visualization dye and for separation gel is used

  • Soniya Shekhawat
    August 13, 2025

    Done sir👍

  • Mohd juber Ali
    August 14, 2025

    Option (d)
    All option right

  • shruti sharma
    August 14, 2025

    option d is correct

  • Dharmpal Swami
    August 14, 2025

    For gel electrophoresis –
    1.negative charge migrate in gel
    2.Et. br. to detect dna in gel
    3.Agr.and PAGE separate dna based on size

  • Priya dhakad
    August 14, 2025

    Option d is correct
    All 3 points are important for gel electrophoresis

  • Mansukh Kapoor
    August 14, 2025

    The correct answer is option 4th because Gel electrophoresis mainly separates the DNA and RNA on the basis of charge and size . So all the points gives the correct explanation of this.

  • Savita Garwa
    August 14, 2025

    D is correct answer

  • Anjani sharma
    August 14, 2025

    Answer 4 all of them are correct

  • Akshita Saini
    August 14, 2025

    Correct ans D

  • Pratibha sethiya
    August 14, 2025

    Correct answer is 4
    All of the above

  • Aafreen
    August 14, 2025

    Ans-4 All are correct….
    1) Negative charge migrates towards positive electrode
    2) Dye-used to stain DNA in the gel
    3) Leading to separation based on size.

  • Dipti Sharma
    August 14, 2025

    All of the above is necessary

    • Vijay kumar Meena
      August 24, 2025

      4 ✔️

  • Neelam Sharma
    August 14, 2025

    All of the above

  • Karishma
    August 14, 2025

    all of the above is imp for gel electro

  • Krishna Tripathi
    August 14, 2025

    The answer is option D all of the above are necessary for gel electrophoresis.

  • Priti khandal
    August 14, 2025

    yes sir d option is a right

  • Priya khandal
    August 14, 2025

    All of the above are important for gel electrophoresis

  • Roopal Sharma
    August 14, 2025

    ll of the above are important for gel electrophoresis

  • Deepika Sheoran
    August 15, 2025

    All of the above is necessary for gel electrophoresis.

  • Santosh Saini
    August 15, 2025

    gel electrophoresis = -ve charge nucleic acid to migrate through the gel , EtBr used for detection of DNA , agarose or polyacryl amide to separate the DNA boase on size, so 4th option is correct

  • Shivani
    August 15, 2025

    All of the above are important .

  • Nilofar Khan
    August 16, 2025

    Correct answer is d

  • payal
    August 17, 2025

    Answer d all of the above are important for gel electrophoresis

  • Manisha choudhary
    August 17, 2025

    Gel m DNA and RNA electric field m move krte h
    Separation size and shape k base p hota h
    Sds page m separation size shape k base p hoga
    Page m protein p natural charge size shape k base p separate hoga
    So 4th option is right

    • Manisha choudhary
      August 17, 2025

      Srry
      Sds detergent h Sds protein ko denature kr deta and uniformly negative charge provide krta h so y bs size k base p sepration allow krta h shape and charge k base p nhi

    • Manisha choudhary
      August 17, 2025

      Srry sds detergent hso y protein ko denature kr deta h and uniformly negative charge provide krta hai
      Y size k base p sepration allow krta h shape and charge k base p nhi

  • Kavita choudhary
    August 17, 2025

    All of the above important for gel electrophoresis

  • Surbhi Rajawat
    August 17, 2025

    A particular gel matrix like agarose or polyacrylamide and the negative charge on DNA are important to make it run in the gel and then etbr like dyes are used to visualise the DNA. Hence all three points are correct

  • Kajal
    August 17, 2025

    All of these are imp. For gel electrophoresis

  • Avni
    August 17, 2025

    (d) All of the above are important for gel electrophoresis

  • Khushi Vaishnav
    August 17, 2025

    All above 4 are needed.

  • Sakshi
    August 17, 2025

    “D” option is correct
    Because all the above factors necessary for gel electrophoresis

  • Shrddha Modi
    August 17, 2025

    D is correct option

  • Meenakshi Choudhary
    August 18, 2025

    all three factors are essential for gel electrophoresis

  • Simran Saini
    August 18, 2025

    All of the above are important for gel electrophoresis.

  • Varsha Tatla
    August 19, 2025

    Given all information req. For gel electoforasis

  • Komal Sharma
    August 21, 2025

    (d) All of the above are important for gel electrophoresis.

  • Hanshika Bansal
    August 23, 2025

    Done sir

  • Bhavana kankhedia
    August 24, 2025

    all of the above are important for gel electrophoresis

  • Priyanka Choudhary
    August 24, 2025

    Correct answer is option d,all options are right answers,Gel electrophoresis works by separating nucleic acids ,DNA/RNA,or proteins based on size and charge

  • Bharti Yadav
    August 24, 2025

    All of the above are important for gel electrophoresis

  • Urmi sisodiya
    August 24, 2025

    For gel electrophoresis all 4 is needed that is ethidium or polyacrylamide gel for seperation of dna rna, visualization of bands in gel

  • Priyanka Choudhary
    August 24, 2025

    Option d is correct all of the above,
    Because tino hi important h
    Charge movement
    Gel separation
    Dye visualization

  • Sonam Saini
    August 24, 2025

    Option d is right

  • Ankita Pareek
    August 24, 2025

    All the options are correct

  • Alec
    August 24, 2025

    option d – all of the above are necessary.

  • Niti Tanwar
    August 24, 2025

    Option d is right

  • Anjali
    August 24, 2025

    All of the above

  • Falguni Prakash
    August 24, 2025

    Option D all are correct

  • Devika
    August 25, 2025

    All of the above are necessary for gel electrophoresis
    Option .d

  • Kashish yadav
    August 25, 2025

    Ok sir

  • sakshi khatri
    August 25, 2025

    d all

  • Shivani Panwar
    August 25, 2025

    All of the above are important for gel electrophoresis 👍

    • Monika meena
      August 27, 2025

      4 option is correct 1 .) charge movement 2.) etbr to provide a means to visualise the dna in gel 3.) agarose gel or polyacrylamide separate the dna based on their size

  • Surendra Doodi
    August 27, 2025

    Negative charg nucleic acid
    Etbr to provide a visualization dna in the gel
    Agrose and page based on the size

  • Pratibha Jain
    August 30, 2025

    Correct answer is option (4)
    Negative charge migrates towards positive electrode
    Dye-used to stain DNA in the gel
    Leading to separation based on size.

  • Sheikh Aleen
    August 30, 2025

    Answer d all of the above are important for gel electrophoresis

  • Anisha Beniwal
    August 30, 2025

    All of the above

  • Vanshika Sharma
    August 30, 2025

    All of the above are imp for gel electrophoresis

  • Akshita Jangid
    August 31, 2025

    Gel electrophoresis works by separating nucleic acids or proteins based on charge and molecular weight

  • Shobha Kanwar
    August 31, 2025

    Option (d)All option are right

  • Asha Gurzzar
    August 31, 2025

    D is correct all of the above

  • Ajay Sharma
    September 2, 2025

    All are required for gel to work

  • Anisha jakhar
    September 3, 2025

    (d) All of the above are important for gel electrophoresis.

  • Muskan Yadav
    September 3, 2025

    All of the above are important for gel electrophoresis.

  • Somya Yadav
    September 5, 2025

    All of the above

  • Aakansha sharma Sharma
    October 1, 2025

    The correct answer is:

    (d) All of the above are important for gel electrophoresis

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