Which of the following is important for gel electrophoresis to work?
a. Negatively charged nucleic acids to migrate through the gel.
b. Ethidium bromide to provide a means to visualize the DNA in the gel.
c. Agarose or polyacrylamide to separate the DNA based on size.
d. All of the above are important for gel electrophoresis
The correct answer is:
(d) All of the above are important for gel electrophoresis.
Explanation:
Gel electrophoresis works by separating nucleic acids (DNA/RNA) or proteins based on size and charge. Each option listed is crucial for the process:
-
(a) Negatively charged nucleic acids to migrate through the gel
- DNA and RNA are negatively charged due to their phosphate backbone.
- Under an electric field, they migrate toward the positive electrode (anode).
-
(b) Ethidium bromide (EtBr) to provide a means to visualize the DNA in the gel
- EtBr is a fluorescent dye that intercalates between DNA bases.
- Under UV light, it makes DNA bands visible.
- Alternative dyes like SYBR Safe or GelRed can also be used.
-
(c) Agarose or polyacrylamide to separate the DNA based on size
- Agarose gel is used for larger DNA fragments (100 bp to several kb).
- Polyacrylamide gel is used for smaller DNA or RNA fragments and provides higher resolution.
- The gel matrix acts like a sieve, allowing smaller fragments to migrate faster than larger ones.
Conclusion:
Since all three factors are essential for gel electrophoresis to function correctly, the correct answer is:
(d) All of the above are important for gel electrophoresis.



97 Comments
Parul
February 25, 2025Okay sir able to do it and understood the importance and difference between knowledge available in books and provided by you in classes.
Alina mansuri
February 25, 2025Got it
Mahima Sharma
August 24, 2025Negative charg nucleic acid
Etbr to provide a visualization dna in the gel
Agrose and page based on the size
Ujjwal
February 25, 2025Good sir
Ritika Jangir
February 26, 2025Done
Shreeji Charan
February 26, 2025Was correct
Lavanya Sharma
February 26, 2025Was correct sir
Suman bhakar
February 26, 2025Done
Ashwini Shekhawat
February 26, 2025To the point explanation ✨
Lokesh kumawat
February 26, 2025Done
Abhilasha
February 26, 2025Done
Arushi
February 26, 2025☑️👍
Shivani meena
February 26, 2025Done ✅
Vikram
February 26, 2025👍🏼
Vikram
February 26, 2025👍🏼👍🏻
Vikram
February 26, 2025🆗
Jyoti Meena
February 27, 2025Done
Pooja jat
February 27, 2025Nice to see point to point nd easy way of explanation.
Kabeer Narwal
February 27, 2025👍👍
Prami Masih
February 27, 2025Okay sir
yogesh sharma
February 27, 2025Great explanation sir 🙏
Nisha
February 27, 2025Nice way explantion
yogesh sharma
February 27, 2025Got it sir
Priya khandal
March 2, 2025Done sir
Sumit Sultan
March 3, 2025👍👍💫
Anita choudhary
April 18, 2025Done 👍✅
Anurag Giri
August 13, 2025Ans is 4 Negatively charged nucleic acids to migrate through the gel towards positive electrode and Ethidium bromide (EtBr) to provide a means to visualize the DNA in the gel under uv light and agarose gel used for separation of larger dna frag. And polysacc. Use for small dna frag
Khushi Agarwal
August 13, 2025Option 4 is correct here
Tino point milke hi gel electrophoresis kaam karta hai
Charge movement ke liye,
Gel separation ke liye,
Dye visualization ke liye.
Aman Choudhary
August 13, 2025option d is correct All of the above are important for gel electrophoresis
for movement charge is used, for visualization dye and for separation gel is used
Soniya Shekhawat
August 13, 2025Done sir👍
Mohd juber Ali
August 14, 2025Option (d)
All option right
shruti sharma
August 14, 2025option d is correct
Dharmpal Swami
August 14, 2025For gel electrophoresis –
1.negative charge migrate in gel
2.Et. br. to detect dna in gel
3.Agr.and PAGE separate dna based on size
Priya dhakad
August 14, 2025Option d is correct
All 3 points are important for gel electrophoresis
Mansukh Kapoor
August 14, 2025The correct answer is option 4th because Gel electrophoresis mainly separates the DNA and RNA on the basis of charge and size . So all the points gives the correct explanation of this.
Savita Garwa
August 14, 2025D is correct answer
Anjani sharma
August 14, 2025Answer 4 all of them are correct
Akshita Saini
August 14, 2025Correct ans D
Pratibha sethiya
August 14, 2025Correct answer is 4
All of the above
Aafreen
August 14, 2025Ans-4 All are correct….
1) Negative charge migrates towards positive electrode
2) Dye-used to stain DNA in the gel
3) Leading to separation based on size.
Dipti Sharma
August 14, 2025All of the above is necessary
Vijay kumar Meena
August 24, 20254 ✔️
Neelam Sharma
August 14, 2025All of the above
Karishma
August 14, 2025all of the above is imp for gel electro
Krishna Tripathi
August 14, 2025The answer is option D all of the above are necessary for gel electrophoresis.
Priti khandal
August 14, 2025yes sir d option is a right
Priya khandal
August 14, 2025All of the above are important for gel electrophoresis
Roopal Sharma
August 14, 2025ll of the above are important for gel electrophoresis
Deepika Sheoran
August 15, 2025All of the above is necessary for gel electrophoresis.
Santosh Saini
August 15, 2025gel electrophoresis = -ve charge nucleic acid to migrate through the gel , EtBr used for detection of DNA , agarose or polyacryl amide to separate the DNA boase on size, so 4th option is correct
Shivani
August 15, 2025All of the above are important .
Nilofar Khan
August 16, 2025Correct answer is d
payal
August 17, 2025Answer d all of the above are important for gel electrophoresis
Manisha choudhary
August 17, 2025Gel m DNA and RNA electric field m move krte h
Separation size and shape k base p hota h
Sds page m separation size shape k base p hoga
Page m protein p natural charge size shape k base p separate hoga
So 4th option is right
Manisha choudhary
August 17, 2025Srry
Sds detergent h Sds protein ko denature kr deta and uniformly negative charge provide krta h so y bs size k base p sepration allow krta h shape and charge k base p nhi
Manisha choudhary
August 17, 2025Srry sds detergent hso y protein ko denature kr deta h and uniformly negative charge provide krta hai
Y size k base p sepration allow krta h shape and charge k base p nhi
Kavita choudhary
August 17, 2025All of the above important for gel electrophoresis
Surbhi Rajawat
August 17, 2025A particular gel matrix like agarose or polyacrylamide and the negative charge on DNA are important to make it run in the gel and then etbr like dyes are used to visualise the DNA. Hence all three points are correct
Kajal
August 17, 2025All of these are imp. For gel electrophoresis
Avni
August 17, 2025(d) All of the above are important for gel electrophoresis
Khushi Vaishnav
August 17, 2025All above 4 are needed.
Sakshi
August 17, 2025“D” option is correct
Because all the above factors necessary for gel electrophoresis
Shrddha Modi
August 17, 2025D is correct option
Meenakshi Choudhary
August 18, 2025all three factors are essential for gel electrophoresis
Simran Saini
August 18, 2025All of the above are important for gel electrophoresis.
Varsha Tatla
August 19, 2025Given all information req. For gel electoforasis
Komal Sharma
August 21, 2025(d) All of the above are important for gel electrophoresis.
Hanshika Bansal
August 23, 2025Done sir
Bhavana kankhedia
August 24, 2025all of the above are important for gel electrophoresis
Priyanka Choudhary
August 24, 2025Correct answer is option d,all options are right answers,Gel electrophoresis works by separating nucleic acids ,DNA/RNA,or proteins based on size and charge
Bharti Yadav
August 24, 2025All of the above are important for gel electrophoresis
Urmi sisodiya
August 24, 2025For gel electrophoresis all 4 is needed that is ethidium or polyacrylamide gel for seperation of dna rna, visualization of bands in gel
Priyanka Choudhary
August 24, 2025Option d is correct all of the above,
Because tino hi important h
Charge movement
Gel separation
Dye visualization
Sonam Saini
August 24, 2025Option d is right
Ankita Pareek
August 24, 2025All the options are correct
Alec
August 24, 2025option d – all of the above are necessary.
Niti Tanwar
August 24, 2025Option d is right
Anjali
August 24, 2025All of the above
Falguni Prakash
August 24, 2025Option D all are correct
Devika
August 25, 2025All of the above are necessary for gel electrophoresis
Option .d
Kashish yadav
August 25, 2025Ok sir
sakshi khatri
August 25, 2025d all
Shivani Panwar
August 25, 2025All of the above are important for gel electrophoresis 👍
Monika meena
August 27, 20254 option is correct 1 .) charge movement 2.) etbr to provide a means to visualise the dna in gel 3.) agarose gel or polyacrylamide separate the dna based on their size
Surendra Doodi
August 27, 2025Negative charg nucleic acid
Etbr to provide a visualization dna in the gel
Agrose and page based on the size
Pratibha Jain
August 30, 2025Correct answer is option (4)
Negative charge migrates towards positive electrode
Dye-used to stain DNA in the gel
Leading to separation based on size.
Sheikh Aleen
August 30, 2025Answer d all of the above are important for gel electrophoresis
Anisha Beniwal
August 30, 2025All of the above
Vanshika Sharma
August 30, 2025All of the above are imp for gel electrophoresis
Akshita Jangid
August 31, 2025Gel electrophoresis works by separating nucleic acids or proteins based on charge and molecular weight
Shobha Kanwar
August 31, 2025Option (d)All option are right
Asha Gurzzar
August 31, 2025D is correct all of the above
Ajay Sharma
September 2, 2025All are required for gel to work
Anisha jakhar
September 3, 2025(d) All of the above are important for gel electrophoresis.
Muskan Yadav
September 3, 2025All of the above are important for gel electrophoresis.
Somya Yadav
September 5, 2025All of the above
Aakansha sharma Sharma
October 1, 2025The correct answer is:
(d) All of the above are important for gel electrophoresis