Glycerol is added to protein samples before they are loaded to the wells of PAGE. The function of glycerol is to
(a) Stabilize protein structure
(b) Provide density to the sample
(c) Both A & B
(d) Helps to reduce disulphide bonds by b- mercapto ethanol
The correct answer is:
(b) Provide density to the sample
Explanation:
Glycerol is added to protein samples before loading onto polyacrylamide gel electrophoresis (PAGE) for the following reason:
- Increased Density: Glycerol makes the sample denser than the running buffer, allowing it to sink into the well rather than diffusing into the buffer. This ensures proper loading and prevents sample loss.
Why not the other options?
- (a) Stabilize protein structure → Glycerol can stabilize proteins in some cases, but in PAGE, it primarily helps in loading, not structure stabilization.
- (c) Both A & B → While glycerol can have stabilizing effects in other applications, its main role in PAGE is to increase sample density, making option (b) the best choice.
- (d) Helps to reduce disulfide bonds by β-mercaptoethanol → β-mercaptoethanol itself reduces disulfide bonds, not glycerol.
Thus, option (b) “Provide density to the sample” is the correct answer. ✅
77 Comments
Suman bhakar
February 26, 2025Done
Mahima Sharma
August 24, 2025Provide density to sample
Arushi
February 26, 2025☑️👍
Parul
February 27, 2025Easily done. Understood sir
Pooja jat
February 27, 2025Easy one .,, nicely understood.
Lavanya Sharma
February 27, 2025Solved sir!
Lavanya Sharma
February 27, 2025Perfectly did it !
Prami Masih
February 27, 2025Very nice sir
Ujjwal
February 27, 2025Done sir
Nisha
February 27, 2025Done
yogesh sharma
February 27, 2025Done sir
Akshay mahawar
February 28, 2025Done👍
Lokesh kumawat
March 1, 2025Done
Abhilasha
March 1, 2025Right ✅
Shalu Choudhary
March 2, 2025Done sir
VIKRAM GAHLOT
March 7, 2025👍
Anita choudhary
April 19, 2025👍☑️
Aman Choudhary
August 13, 2025option b is correct glycerol make protein sample denser by which the band show clear
Khushi Agarwal
August 14, 2025Option b is correct
Glycerol heavy hota hai, isliye wo sample ka density badha deta hai, taaki wo seedha neeche well ke bottom me settle ho jaye aur electrophoresis start hone par achhi tarah migrate kare.
Dharmpal Swami
August 14, 2025Provide density to the sample
Neelam Sharma
August 14, 2025Provide density to the sample
Anjani sharma
August 14, 2025Provide density to the sample in electrophoresis ,so answer b
Karishma Don
August 14, 2025it provides the density to the proteins
Deekshita Khandelwal
August 14, 2025Easy understood
Deekshita Khandelwal
August 14, 2025Easy understood, nice explaination
Mitali
August 14, 2025option (b) “Provide density to the sample” is the correct answer. ✅
Roopal Sharma
August 14, 2025Provide density to sample
Priya dhakad
August 14, 2025Provide density to the sample option b is correct
Mohd juber Ali
August 14, 2025Option (b) glycerol provide density to the sample and prevent sample loss
Mansukh Kapoor
August 14, 2025The correct answer is option 2nd
Provides density to the sample because when glycerol is added in it then it increases density of the sample
Deepika Sheoran
August 14, 2025Option b is correct.Provide density to the sample.
Sheikh Aleen
August 21, 2025Provide density to the sample
Dipti Sharma
August 14, 2025Glycerol Increase Density of sample.
Sneha Kumawat
August 14, 2025Glycerol Provide density to the sample
Monika jangid
August 14, 2025Glycerol Provide density to the sample
Surbhi Rajawat
August 14, 2025Glycerol addition makes the sample denser therefore it runs slowly in the gel and separation is better
Priya khandal
August 14, 2025Provide density to the sample
Shivani
August 15, 2025(b) increase density of sample
Kajal
August 15, 2025Option b
Provide density to the sample
shruti sharma
August 15, 2025provide density to the sample
Kavita choudhary
August 17, 2025Provide density of the sample
Aafreen
August 17, 2025Provide density to the sample bcoz glycerol addition makes sample denser
Avni
August 17, 2025Glycerol provide density to the sample
Neeraj Sharma
August 17, 2025Option 2 is correct as glycerol will provide density to the sample so the sample can run in page easily
Simran Saini
August 18, 2025Provide density to the sample.
Sheikh Aleen
August 21, 2025Provide density to the sample
Varsha Tatla
August 23, 2025In SDS , glycerol provide density to sample .it insure proper loading nd prevent sample loss
Bharti Yadav
August 24, 2025Provide density to the sample
Priyanka Choudhary
August 24, 2025Option b,Provide density to the sample is right answer
MOHIT AKHAND
August 24, 2025(B)
Done sir ✅
Anil Choudhary
August 24, 2025Provide density to the sample
Bhavana kankhedia
August 24, 2025Provide density to the sample
Bhavana kankhedia
August 24, 2025Provide density to sample
Alec
August 24, 2025glycerol is added to provide density to the protein sample
Sonam Saini
August 24, 2025Option d is right
Niti Tanwar
August 24, 2025It provide density to the sample
Anjali
August 24, 2025Provide density to the sample
Anju
August 24, 2025Ans:b
Glycerol is viscous so it make sample denser
Seema
August 24, 2025Glycerol is added to increase density of the sample .
Devika
August 24, 2025Option b
Provide density to sample
Falguni Prakash
August 24, 2025Density
Kashish yadav
August 25, 2025Provide density to the sample
Ayush Dubey
August 25, 2025Provide density to the sample
sakshi khatri
August 25, 2025done
Komal Sharma
August 25, 2025Glycerol makes the sample denser than the running buffer, allowing it to sink into the well rather than diffusing into the buffer. This ensures proper loading and prevents sample loss. So option B is correct
Pratibha Jain
August 30, 2025The correct answer is option (b)
Provide density to the sample
Shriyanshi verma
August 30, 2025Easy one .,, nicely understood
Surendra Doodi
August 30, 2025Provide density to the sample
Patel Radha
August 30, 2025(b) Provide density to the sample
Vanshika Sharma
August 31, 2025Provide density to the sample
Shobha Kanwar
August 31, 2025Option b is correct as it Provide density to the sample.
Aartii sharma
September 1, 2025Glycerol added to sample because it provides density to the sample
Anisha jakhar
September 3, 2025The correct option is (b) Provide density to the sample
Muskan Yadav
September 3, 2025Provide density to the sample
Somya Yadav
September 5, 2025Provide density to sample
Meera Gurjar
September 13, 2025Provide density
Aakansha sharma Sharma
October 1, 2025The correct answer is:
(b) Provide density to the sample