Which technique is the exception for the evaluation of protein-protein interaction:
A. Far-Western blotting
B. Chromatin immune precipitation
C. Yeast-two hybrid system
D. Co-immuno precipitation
Which Technique is the Exception for Evaluating Protein-Protein Interactions?
Protein-protein interactions (PPIs) play a crucial role in various biological processes. Several techniques help scientists evaluate these interactions. However, among the following methods, one is an exception:
- Far-Western blotting
- Chromatin Immunoprecipitation (ChIP)
- Yeast-two hybrid system
- Co-immunoprecipitation (Co-IP)
Identifying the Exception
Among these techniques, Chromatin Immunoprecipitation (ChIP) is the exception because it primarily evaluates protein-DNA interactions rather than protein-protein interactions.
Explanation of Each Technique
1. Far-Western Blotting
This technique detects protein-protein interactions using a labeled probe protein instead of an antibody. It is useful for identifying novel binding partners.
2. Chromatin Immunoprecipitation (ChIP) (Exception)
ChIP is designed to study protein-DNA interactions, helping identify where proteins like transcription factors bind on the genome. It is not used for direct protein-protein interaction studies.
3. Yeast-Two Hybrid System
This method detects direct physical interactions between proteins in a yeast-based genetic system. It is widely used for screening novel interacting partners.
4. Co-Immunoprecipitation (Co-IP)
Co-IP is a gold-standard technique for studying protein-protein interactions by using an antibody to pull down protein complexes from cell extracts.
Conclusion
While Far-Western blotting, Yeast-two hybrid, and Co-IP are well-established methods for studying protein-protein interactions, Chromatin Immunoprecipitation (ChIP) is the exception as it focuses on protein-DNA interactions. Understanding the right techniques for studying biological interactions is essential for accurate research outcomes.
52 Comments
Prami Masih
March 4, 2025Done sir
Akshay mahawar
March 5, 2025Easy 👍
Suman bhakar
March 5, 2025👍👍
Abhilasha
March 7, 2025Done ✅
Khushi Mehra
August 20, 2025Answer is B
Chromatin immune precipitation
Varsha Tatla
August 20, 2025Clear
Priya choudhary
August 20, 2025Right answer is option b
Neelam Sharma
August 20, 2025Chromatin immunoprecipitation is exception because it is DNA protein interaction based
Mohd juber Ali
August 21, 2025Understood by solution (b)
Chip= protein -dna intrection not protein protein
Manisha
August 21, 2025Chromatin immuno precipitation used in protein dna interaction
Manisha
August 21, 2025Chromatin immuno precipitation used in protein dna interaction …
Santosh Saini
August 21, 2025Far western blotting to detect protein protein interaction
Khushi Vaishnav
August 21, 2025Chromatin Immunoprecipitation (ChIP) is the exception
Aafreen Khan
August 21, 2025Chromatin Immunoprecipitation (ChIP) is the exception as it focuses on protein-DNA interactions
Anurag Giri
August 22, 2025Far-Western Blotting
This technique detects protein-protein interactions
Sakshi Kanwar
August 22, 2025B far blot is used for Protein protein interaction where as Chromatin Immunoprecipitation used in protein – DNA interaction
Soniya Shekhawat
August 22, 2025Chromatin immuno precipitation is a focus on protein DNA interaction. And remaining all are the protein protein interaction.
Simran Saini
August 22, 2025Chromatin immune precipitation.
Parul Yadav
August 22, 2025Chromatin – Immunoprecipitation (ChIP) is the exception for the evaluation of protein-protein interaction.
Arushi
August 22, 2025Chromatin immuno precipitation
Kirti agarwal
August 23, 2025CHIP
Priya dhakad
August 23, 2025Option b is correct ChIP is designed to study protein-DNA interactions. It is not used for direct protein-protein interaction.
Priya khandal
August 23, 2025Chromatun immune precipitation
Manisha gujar
August 23, 2025B is correct
Akshita Jangid
August 23, 2025Chromatin Immunoprecipitation is the exception because it is used in Protein- DNA interaction
Bharti Yadav
August 23, 2025Far Western blotting is used for protein protein interaction
Devika
August 24, 2025Chromatin immuno precipitation
Sneha Kumawat
August 24, 2025Chromatin immunoprecipitation technique protein dna interaction ka pta lgane ke liye hoti h
Aakanksha Sharma
August 24, 2025Chip is for dna protein interactions
Himanshi
August 24, 2025Chromatin immune precipitation
Kajal
August 24, 2025CH- IP is the correct one includes dna protein interaction…
Bhavana kankhedia
August 24, 2025Chromatin Immunoprecipitation (ChIP)
Ayush Dubey
August 24, 2025Chromatin immunoprecipitation is the exception
Sakshi yadav
August 24, 2025Far western blotting for protein protein interaction
Priyanka Verma
August 24, 2025Chromatin immunoprecipitation is the exception because it detects the protein DNA interaction not protein protein interaction
Sonam Saini
August 24, 2025Far western blotting for protein protein interactions
Surendra Doodi
August 24, 2025🤔
Falguni Prakash
August 24, 2025Chip
Kajal
August 25, 2025Far western blotting
Priyanka Meena
August 26, 2025Chromotin immunopreciptiation
Muskan Yadav
August 26, 2025Chromatin immuno precipitation used in protein dna interaction.
Neha Yadav
August 27, 2025Chromatin immunoprecipitation ( DNA – Protein interactions rather than protein- protein – protein interactions )
Manisha choudhary
August 27, 2025Yeast two hybrid,co- immunoprecipitation( Ci) , far western protein protein interaction k liye hota h chromatin immuno precipitation (Chip) DNA protein interaction k detection k liye hota h
Shriyanshi verma
August 30, 2025Chromatin immuno precipitation used in protein dna interaction
Asha Gurzzar
August 30, 2025B will be exceptional because chromatin immuno precipitation used in protein dna interaction
Alec
August 30, 2025chromatin immuno precipitation is used in protein-dna interaction.
Pratibha Jain
August 30, 2025Chromatin Immunoprecipitation (ChIP) is the exception as it focuses on protein-DNA interactions
anjani sharma
August 30, 2025Chromatin – Immunoprecipitation (ChIP) is the exception for the evaluation of protein-protein interaction.
Anisha jakhar
August 31, 2025Chromatin Immunoprecipitation (ChIP) is the exception because it primarily evaluates protein-DNA interactions rather than protein-protein interactions.
Shobha Kanwar
August 31, 2025Chromatin Immunoprecipitation (ChIP) is the exception
Kanica Sunwalka
August 31, 2025ChIP – focus on protein dna interaction
Anjali
September 1, 2025chromatin immuno precipitation is used in protein-dna interaction.