Role of Reporter Molecules in DNA Hybridization Assays
How reporter molecule function in a DNA hybridization assay?
a. Enhances the stringency of the hybridization reaction
b. Binds the target DNA to the solid support
c. Aids in base pairing
d. Aids in the detection of probe-target hybrids
How Do Reporter Molecules Function in a DNA Hybridization Assay?
DNA hybridization assays are essential tools in molecular biology used to detect specific nucleic acid sequences. A critical component of these assays is the reporter molecule, which facilitates the detection of hybridized DNA.
The correct answer is:
(D) Aids in the detection of probe-target hybrids
Understanding DNA Hybridization Assays
A DNA hybridization assay involves the binding of a labeled DNA or RNA probe to a complementary target sequence. This process is commonly used in genetic testing, infectious disease detection, and forensic analysis.
-
Sample Preparation
- The DNA sample is extracted and immobilized on a solid support (e.g., a membrane or a microarray slide).
- The DNA is denatured into single strands to allow hybridization.
-
Probe Hybridization
- A labeled probe (a complementary sequence of DNA or RNA) is introduced.
- The probe binds to its specific target sequence through complementary base pairing (A-T, G-C).
-
Detection with Reporter Molecule
- The reporter molecule is attached to the probe or a secondary detection system.
- If hybridization occurs, the reporter molecule emits a detectable signal (e.g., fluorescence, chemiluminescence, or colorimetric signals).
Types of Reporter Molecules
-
Radioactive Labels (e.g., P-32-labeled probes)
- Emit radiation, which is detected using autoradiography.
-
Fluorescent Dyes (e.g., Cy3, Cy5, FAM)
- Emit fluorescent signals under specific light wavelengths.
-
Enzyme-Based Reporters (e.g., Horseradish Peroxidase (HRP), Alkaline Phosphatase (AP))
- Convert colorless substrates into visible color changes.
-
Biotin-Streptavidin System
- Biotin-labeled probes bind streptavidin-conjugated enzymes, enhancing signal detection.
Why the Other Options Are Incorrect
-
(A) Enhances the stringency of the hybridization reaction
- Stringency is controlled by temperature, salt concentration, and washing steps, not reporter molecules.
-
(B) Binds the target DNA to the solid support
- DNA binding is facilitated by membranes or microarray slides, not reporter molecules.
-
(C) Aids in base pairing
- Hybridization occurs naturally through hydrogen bonding, not due to reporter molecules.
Conclusion
Reporter molecules in DNA hybridization assays play a crucial role in detecting probe-target hybrids. By generating detectable signals, they allow scientists to confirm hybridization events, making them essential for genetic diagnostics, pathogen detection, and biomedical research.
45 Comments
Ujjwal
March 8, 2025Done sir
Suman bhakar
March 8, 2025Ok sir
Neelam Sharma
August 20, 2025Aids in the detection of probe-target hybrids- reporter molecule
Khushi Mehra
August 20, 2025Reporter molecule detect whether hybridization occur or not by the probe with its complementary DNA sequence
Mansukh Kapoor
August 20, 2025The correct answer is option 4th
DNA hybridization assay aids to detection of the DNA probe target hybrids.
Mohd juber Ali
August 21, 2025(4) reporter molecule function in dna hybridization assay by Aids to detect of probe target hybrid
Aman Choudhary
August 21, 2025Option D is correct
Aids in the detection of probe target hybrids
Khushi Vaishnav
August 21, 2025Aids in the detection of probe-target hybrids
Aafreen Khan
August 21, 2025Aids in the detection of probe-target hybrids
Manisha gujar
August 21, 2025Aids in the detection of the probe target hybrid
Manisha gujar
August 21, 2025Option d
Santosh Saini
August 21, 2025Aids in the detection of the probe target hybrid
Divya rani
August 21, 2025In dna hybridization or Southern blotting reporter molecule facilitate the detection of probe target sequence or hybrid.
Parul Yadav
August 21, 2025Reporter molecule is attached to the probe. If hybridization occur , the reporter molecule emits a detectable signal.
Sakshi Kanwar
August 22, 2025Reported molecule binds to probe in form of radioactive label , fluorescent dye for the detection of hybridized DNA
Priyanka choudhary
August 22, 2025Option D is correct answer
Aids in the detection of probe target hybrids
karishma
August 22, 2025ids in the detection of probe-target hybrids
Soniya Shekhawat
August 23, 2025Reporter molecule which is the labelled probe its emits radiation which is detected by auto radio graphy and it in its fluorescent to detect the hybridised DNA.
Priya dhakad
August 23, 2025Reporter molecules in DNA hybridization assays play a crucial role in detecting probe-target hybrids. So the correct option is d.
Priya khandal
August 23, 2025Acid in the detection of Cprobe target hybrids
Bharti Yadav
August 23, 2025Reporter molecules detect probe-target hybrids in DNA hybridization assays
Yogita
August 24, 2025Aids in the detection of probe – target hybrids
Sneha Kumawat
August 24, 2025Reporter molecule , hybridization ke baad proof target hybrid ka pata lagana hai
Kajal
August 24, 2025Report molecule helps in detection of probe target hybrid it binds with probe n emit detectable signals. Such as florosence……etc
Shivani
August 24, 2025Reporter molecules in DNA hybridization assays play a crucial role in detecting probe-target hybrids. So option D. is correct
Neeraj Sharma
August 24, 2025Reporter molecule aids on the target-probe site
Sakshi yadav
August 24, 2025Option D -Aids in the detection of probe-target hybrids
Surendra Doodi
August 24, 2025Aids in the detection of probe-target hybrids- reporter molecule
Sonam Saini
August 24, 2025Opetion d is right answer
Priyanka Verma
August 24, 2025Option 4 is correct
Falguni Prakash
August 24, 2025Detection of probe target hybrids
Kashish yadav
August 25, 2025Option d
Kajal
August 25, 2025Option d is right
Priyanka Meena
August 26, 2025Detection of probe target hybrid
Muskan Yadav
August 26, 2025Aids in the detection of probe-target hybrids.
Neha Yadav
August 27, 2025Reporter molecule detect the probe- target hybrids by generating detectable signal
Manisha choudhary
August 27, 2025Option D. is correct repoter molecule probe – target hybrid ko detect krta h
Shriyanshi verma
August 30, 2025Reported molecule binds to probe in form of radioactive label , fluorescent dye for the detection of hybridized DNA
Asha Gurzzar
August 30, 2025Aids in the detection of probe target hybrid
Alec
August 30, 2025Aids in the detection of probe target hybrids.
Pratibha Jain
August 30, 2025The correct answer is option (D)
Aids in the detection of probe-target hybrids
Kirti agarwal
August 30, 2025Detection of probe target hybrids
Shobha Kanwar
August 31, 2025Option D is correct
Aids in the detection of probe target hybrids
Kanica Sunwalka
August 31, 2025aids in detection of probe target hybrids
Anjali
September 1, 2025Aids in the detection of probe-target hybrids