Chymotrypsinogen in the native state and reduced unfolded sates is loaded in well A and B respectively. They are
electrophoreses and stain, one of the following results is likely to be observed.
a. Protein in well A would have moved longer distance than that in well B
b. Protein in well A would have moved shorter distance than that in well B
c. Protein in well A and B would have moved the same distance
d. Protein in well A and B would not move at all
The correct answer is:
✅ (b) Protein in well A would have moved a shorter distance than that in well B
Explanation:
Chymotrypsinogen is a globular protein that can exist in:
- Native (folded) state → More compact structure
- Reduced (unfolded) state → Fully extended structure due to the disruption of disulfide bonds
In SDS-PAGE, proteins migrate based on their size (molecular weight), but shape also plays a role in migration distance.
-
Well A (Native Chymotrypsinogen)
- In its folded state, the protein remains more compact.
- This results in less interaction with SDS, leading to less migration.
-
Well B (Reduced & Unfolded Chymotrypsinogen)
- When the protein is denatured (unfolded) using reducing agents (e.g., β-mercaptoethanol or DTT), the disulfide bonds are broken.
- The protein fully extends, allowing more SDS molecules to bind, giving it a higher charge-to-mass ratio.
- This leads to greater migration (moves farther down the gel) than the native protein.
Why Not the Other Options?
- (a) Protein in well A would have moved a longer distance than that in well B ❌ → Incorrect, because native (compact) proteins move less in SDS-PAGE.
- (c) Protein in well A and B would have moved the same distance ❌ → Incorrect, because folded vs. unfolded states affect migration.
- (d) Protein in well A and B would not move at all ❌ → Incorrect, because SDS imparts a negative charge, allowing proteins to migrate.
Conclusion:
Since the unfolded protein (well B) moves farther than the native protein (well A) in SDS-PAGE, the correct answer is:
✅ (b) Protein in well A would have moved a shorter distance than that in well B.
81 Comments
Parul
February 25, 2025Okay sir. Done
Mahima Sharma
August 24, 2025Protein in well a would have more lager distance than well b
Parul
February 25, 2025Sir answer is A as explained in the video.
Mahima Sharma
August 16, 2025Protein in well a move longer distance to well b
Nisha
February 25, 2025Ok
Lokesh kumawat
February 27, 2025Confused …but I think A is correct answer
Kabeer
February 25, 2025Answer is A
Ritika Jangir
February 26, 2025Done
Shreeji Charan
February 26, 2025Guessed it wrong first
Lavanya Sharma
February 26, 2025Got it right sir.
Suman bhakar
February 26, 2025Right sir
Arushi
February 26, 2025Answer A is ☑️
Abhilasha
February 26, 2025Ok sir
Payal Soni
February 27, 2025Yes sir got it
Pooja jat
February 27, 2025Confused in ans …,, which one is correct.. out of A nd B.
Acc. To this video ans. Is A but in solution B is. Correct.
The language of the question is also twisted.
Prami Masih
February 27, 2025Nice sir
yogesh sharma
February 27, 2025Sir there is a slight confusion in answer
Please explain again 😇
yogesh sharma
February 27, 2025A is the correct answer
VIKRAM GAHLOT
March 5, 2025👍
Anita Choudhary
April 18, 2025answer A is correct
Mohd juber Ali
August 14, 2025Option (b)
Well A -Native(folded) more compact so protein migration is less
Well B -reduced(unfolded) jyada SDS molecule bind honge and migration jyada hoga
Khushi Agarwal
August 14, 2025Here answer A is correct bcz native small hoga to migration fast hoga or unfold ki size badi hogi to migration slow hoga
Snehakumawat
August 14, 2025The correct answer is A bcz native size me small hota h toh fast move krega and unfolded size me large hota h toh slow move krega
Anjani sharma
August 14, 2025Answer 1 cause native will move fast folded then misfolded
Soniya Shekhawat
August 14, 2025Native protein size is small move fast and unfolded protein is move slow due to their large size.
Neelam Sharma
August 14, 2025Protein in well A would have moved a shorter distance than that in well B
Mansukh Kapoor
August 14, 2025The correct answer is option 1st because protein A is in native form so it is small in size then it move faster than the unfolded protein B
Dipti Sharma
August 14, 2025Protein in well A would have moved a shorter distance than that in well B
Karishma
August 14, 2025the folded protein or native is not open with SDS, while the denatured unfolded is exposed more with SDS giving -ve charge which leads to high migration down the gel
Priti khandal
August 14, 2025yes sir a option is right a protein in well A have moved longer distance then that in well B
Priya khandal
August 14, 2025Protein in well a whould have moved shorter distance than that in well b
Shivani
August 15, 2025Protein. In well a moved shorter distance than that in well b because
Shivani
August 15, 2025In sds page protein migration depends on size but shape is also plays an important role
Divya rani
August 16, 20251 is right because in native state well A protein is folded so migrate slow then the reduced unfolded well B protein
Aafreen
August 16, 2025May be A is correct
Divya rani
August 16, 2025A mistake bg me so I correct that the folded will cover more distance than misfolded
Aafreen
August 16, 2025May be A is correct
Payal
August 17, 2025Option b is right native protein(folded) move slow and unfolded protein move fast
Manisha choudhary
August 17, 2025Doubt h sir Answer A hona chahiye
Kyoki native folded jyada move kregi compact hogi
Unfolded slow move kregi
Sds page kaa question m mention hi nhi kiya hua
Ki sds dene s hi unfolded hui h protein
Kavita choudhary
August 17, 2025Answer is c because native protein migrate slow and unfold protein move fast
Manisha gujar
August 17, 2025Compact dna move fast as compare to reduced dna so option a is correct
Manisha gujar
August 17, 2025Compact dna move faster as compare to reduced dna
Manisha gujar
August 17, 2025Option a is correct
Parul Yadav
August 17, 2025Option b is correct.
SDS denatures proteins and coat protein with a uniform negative charge. Unfolding increases surface area for SDS binding. So reduced, unfolded proteins migrate faster than native proteins.
Kajal
August 17, 2025Protein in well A move is folded so move slow then B
Avni
August 17, 2025option (a) is the correct answer
Khushi Vaishnav
August 17, 2025Protein in well A would have moved a shorter distance than that in well B
Meenakshi Choudhary
August 18, 2025A is correct answer
Shrddha Modi
August 18, 2025As condensed moves faster. And reduced moves slow comparatively so and is A
Simran Saini
August 18, 2025Protein in well A would have moved a shorter distance than that in well B.
Varsha tatla
August 19, 2025Clear
Varsha tatla
August 19, 2025Option 1st will be absolutely correct
Komal Sharma
August 21, 2025In SDS-PAGE, proteins migrate based on their size (molecular weight), but shape also plays a role in migration distance.. Since the unfolded protein (well B) moves farther than the native protein (well A) in SDS-PAGE so option A is correct Protein in well A would have moved a shorter distance than that in well B.
Bhavana kankhedia
August 24, 2025Protein in well A would have moved a shorter distance than that in well B
Bharti Yadav
August 24, 2025Protein in well A would have moved a shorter distance than that in well B
Priyanka Choudhary
August 24, 2025Correct answer is option a,because of this native small size m hoga to fast move krega and unfolded size m hoga to large size m hoga and slow move krega
Urmi sisodiya
August 24, 2025Protein a moves slower because of its native form and protein b is present in denatured form result in fast migration
Sonam Saini
August 24, 2025Kuan A mein protein khuaa B ki comparison mein kam duri Tak Chala hoga
Ankita Pareek
August 24, 2025Protein B move farther then in well A because b is unfolded and a is in its native state
Alec
August 24, 2025option b – protein in A will move shorter distance than in B because it is in native state thus having compact structure so resulting in shorter distance covered.
Priya dhakad
August 24, 2025Protein in well A would have moved a shorter distance than that in well B.
Falguni Prakash
August 24, 2025A
sakshi
August 24, 2025Protein in well a would have moved shorter distance then that in well b
Devika
August 25, 2025Protein In wellA is folded so it moves shorter than that of well B
Kashish yadav
August 25, 2025Ok sir
Vijay Marmat
August 25, 2025Done ✔️ 👍
sakshi khatri
August 25, 2025done
Ayush Dubey
August 25, 2025Protein in well A would have moved a shorter distance than that in well B
shruti sharma
August 25, 2025Answer is c because native protein migrate slow and unfold protein move fast
Monika meena
August 27, 2025Protein in well A would have moved longer distance than that in well B ✅
Savita Garwa
August 29, 2025Native protein size is small move fast and unfolded protein is move slow due to their large size.
Sheikh Aleen
August 30, 2025Ans C Protein in well A would have moved a shorter distance than that in well B
Anisha Beniwal
August 30, 2025Protein in well A would have moved longer distance than that in well B
Vanshika Sharma
August 30, 2025Protein in well A would have moved a shorter distance than that wellB
Shobha Kanwar
August 31, 2025Answer a) cause native will move fast folded then misfolded
Akshita Jangid
September 1, 2025Unfolded protein well B moves farther than the native protein well A in SDS-PAGE
Anisha jakhar
September 3, 2025(b) Protein in well A would have moved a shorter distance than that in well B
Muskan Yadav
September 3, 2025Protein in well A would have moved a shorter distance than that in well B
Ajay Sharma
September 4, 2025Option b is correct
Somya Yadav
September 5, 2025Protein in well A would have moved a shorter distance than that in well B.
Aakansha sharma Sharma
October 1, 2025The correct answer is:
✅ (b) Protein in well A would have moved a shorter distance than that in well B