An oligonucleotide bound to a protein migrates slower than the free oligonucleotide in a polyacrylamide gel. If
antibody specific to the oligonucleotide binding protein is bound to this complex, the antibody treated complex
will
a. Migrate slower than both the free oligonucleotide and the untreated complex.
b. Migrate slower than the free oligonucleotide but faster than the untreated complex
c. Migrate at the same rate as the untreated complex
d. Migrate at the same rate as the free oligonucleotide
The correct answer is:
✅ (a) Migrate slower than both the free oligonucleotide and the untreated complex.
Explanation:
In polyacrylamide gel electrophoresis (PAGE), molecules migrate based on size and charge.
-
Free oligonucleotide
- Small and negatively charged → Moves fastest through the gel.
-
Oligonucleotide + Protein Complex
- When a protein binds to the oligonucleotide, it increases the molecular weight and alters charge distribution, making the complex migrate slower than the free oligonucleotide.
-
Oligonucleotide + Protein Complex + Antibody
- When an antibody binds to the protein-oligonucleotide complex, the complex size further increases.
- Larger complexes experience more resistance in the gel matrix, causing them to migrate even slower than the untreated complex.
Why Not the Other Options?
- (b) Migrate slower than the free oligonucleotide but faster than the untreated complex ❌ → Incorrect, because the antibody increases the molecular weight, further reducing mobility.
- (c) Migrate at the same rate as the untreated complex ❌ → Incorrect, because adding the antibody increases size, slowing migration.
- (d) Migrate at the same rate as the free oligonucleotide ❌ → Incorrect, because both protein binding and antibody addition increase molecular size, slowing migration.
Conclusion:
Since the antibody increases the complex’s molecular weight, it will migrate even slower than the untreated complex and the free oligonucleotide.
✅ Correct answer: (a) Migrate slower than both the free oligonucleotide and the untreated complex.



79 Comments
Akshay mahawar
February 25, 2025Done👍
Mahima Sharma
August 24, 2025Migration slower then both the free oligonucleotide and the untreated complex
Nisha
February 25, 2025Ok
BANWARI lal khatana
February 27, 2025Well done 👍
Shreeji Charan
February 26, 2025Was right
Suman bhakar
February 26, 2025Ok
Parul
February 26, 2025Okay sir. Easily understood.
Arushi
February 26, 2025☑️
Arushi
February 26, 2025☑️done
Pooja jat
February 27, 2025Done.
Lavanya Sharma
February 27, 2025Good explaination.
Prami Masih
February 27, 2025Okay sir
Aafreen
August 16, 2025Ans-a
In a polyacrylamide gel electrophoresis (PAGE) the migration rate of a molecule is based on its size and shape.
Ujjwal
February 27, 2025Nice question
Ujjwal
February 27, 2025Done 👍
yogesh sharma
February 27, 2025Easily explained sir
Abhilasha
March 1, 2025Got it sir 👍🏻
Lokesh kumawat
March 1, 2025Done
VIKRAM GAHLOT
March 7, 2025ok sir
Anita Choudhary
April 18, 2025Done
Mohd juber Ali
August 14, 2025Option (a)
Free oligonucleotides ka m.w. kam hota h fast move karta hai if add protein complex with oligonucleotides then m.w. increase and slow migration
Shrddha Modi
August 18, 2025A
Khushi Agarwal
August 14, 2025Option A is correct
Migrate slower than both the free oligonucleotide and the untreated complex.
Free oligo – fastest
Oligo + protein (mw,size ) increase – slower
Oligo + protein+ antibody- slowest
Dharmpal Swami
August 14, 2025PAGE molecules migrate based on size and charge
Aman Choudhary
August 14, 2025option A is correct because by addition of protein and antibody on free oligo slowdown the movement
Sneha Kumawat
August 14, 2025The correct answer is A, when an antibody binds to the protein oligonucleotide complex the complex size further the increases
Neelam Sharma
August 14, 2025Migration slower than both of the free oligonucleotide and untreated complex
Mansukh Kapoor
August 14, 2025The correct answer is option 1st
It moves slower than the free oligonucleotide and the protein complex
Soniya Shekhawat
August 14, 2025Answer is 1st 👍🏻and their explanation is very easy to understand and no one can teach like this that clear all topics in classroom with there all type of question within simultaneously, great Sir 🙏🏻
Dipti Sharma
August 14, 20251. Migrate slower than both the free oligonucleotide and the untreated complex
Manisha gujar
August 14, 2025Slow migration from both free and untreated oligonucleotide
Karishma
August 14, 2025oligo with antibody and protein complex migrates more slower than with oligo+protein complex
Priti khandal
August 14, 2025option A is right because oligonucleotide fast and oligonucleotide +protein slow and oligonucleotide +protein +antibody is slowestm
Priya khandal
August 14, 2025Done sir bahut acche se explain Kiya Hai
Roopal Sharma
August 14, 2025Migrate slower
Shivani
August 15, 2025Option A is correct
Mitali saini
August 16, 2025(a) Migrate slower than both the free oligonucleotide and the untreated complex.
Divya rani
August 16, 2025Binding is increase due to oligonucleotide+protein + antibody so weight and size will also increase then the migration is become very slow so this complex is the slowest.
Payal
August 17, 2025A. Free oligonucleotide move fast M. W. Low and oligonucleotide+protein+antibody move slow M. W. Increase
Sheikh Aleen
August 17, 2025Migrate slower than both the free oligonucleotide and the untreated complex.
Kajal
August 17, 2025Option – a
Migrate slower than both the free oligonucleotide and untreated complex
Avni
August 17, 2025It will migrate slower than both the free Oligonucleotide and untreated complex
Simran Saini
August 18, 2025Migrate slower than both the free Oligonucleotide and the untreated complex.
Shrddha Modi
August 18, 2025Because it Becomes heavier than oligannucleotide and Untreated complex. Option A
Meenakshi Choudhary
August 19, 2025Migrate slower than both the free oligonucleotide and the untreated complex.
Deepika Sheoran
August 19, 2025Slow migration from both the free oligonucleotide and untreated complex.
Manisha choudhary
August 20, 2025Doen sir
Specific protein jo loigonucleotide binding protein complex s bind krta h tooo iska weight free loigonucleotide and loigonucleotide binding protein complexe s jyada hoga too y in dono s hi slow move krega
Answer a hoga
Bhavana kankhedia
August 24, 2025migrate slower than both the free oligonucleotide and the untreated complex.
Bharti Yadav
August 24, 2025Migrate slower than both the free oligonucleotide and the untreated complex.
Priyanka Choudhary
August 24, 2025A is right answer,Migrate slower than both the free oligonucleotide and the untreated complex
Priyanka Choudhary
August 24, 2025Right answer is option a,Migrate slower than both the free oligonucleotide and the untreated complex
Urmi sisodiya
August 24, 2025When antibody binds to Protein and oligonucleotide then the complex size gets increased result in less migration and covered less distance
Sonam Saini
August 24, 2025Option a is right
Alec
August 24, 2025option a – The antibody treated complex will move slower than both the free oligonucleotide and the untreated complex.
Priya dhakad
August 24, 2025Option – a
Migrate slower than both the free oligonucleotide and untreated complex
Anjali
August 24, 2025Ans is a. Migrate slower than both the free oligonucleotide and the untreated complex.
Falguni Prakash
August 24, 2025A
Sakshi
August 24, 2025option a is correct
Migrate slower than both free oligo nucleotide and untreated complex
Devika
August 25, 2025Migrate slower than both the free oligonucleotide and the untreated complex
Kashish yadav
August 25, 2025Ok sir
sakshi khatri
August 25, 2025done
Vijay kumar Meena
August 25, 2025A ✔️
Ayush Dubey
August 25, 2025Migrate slower than both the free oligonucleotide and the untreated complex.
shruti sharma
August 25, 2025Migrate slower than both the free oligonucleotide and the untreated complex
Yogita
August 27, 2025Migration slower than both the free oligonucleotide and the unknown untreated
Monika meena
August 27, 2025Migrate slower than both free oligonucleotide and the untreated complex ✅
Surendra Doodi
August 27, 2025Migrate slower than both the free oligonucleotide and the untreated complex.
Pratibha Jain
August 30, 2025Correct answer is option (a)
Migrate slower than both the free oligonucleotide and the untreated complex.
Anisha Beniwal
August 30, 2025Migrate slower than both the free oligonucleotide and the untreated complex.
anjani sharma
August 30, 2025Answer a
Slow migration from both the free oligonucleotide and untreated complex.
Vanshika Sharma
August 31, 2025Migrate slower in both the free oligonucleotide and untreated complex
Shobha Kanwar
August 31, 2025The correct answer is A, when an antibody binds to the protein oligonucleotide complex the complex size further the increases
Asha Gurzzar
September 1, 2025A is correct
Akshita Jangid
September 1, 2025Migrate slower than both the free oligonucleotide
Anisha jakhar
September 3, 2025(a) Migrate slower than both the free oligonucleotide and the untreated complex.
Muskan Yadav
September 3, 2025Migrate slower than both the free oligonucleotide and the untreated complex.
Ajay Sharma
September 4, 2025Treated complex will become heavier and less faster
Somya Yadav
September 5, 2025Migrate slower than both the free oligonucleotide and the untreated complex.
Aakansha sharma Sharma
October 1, 2025The correct answer is:
✅ (a) Migrate slower than both the free oligonucleotide and the untreated complex.