Which of the following statement about 2D gel electrophoresis is correct?
(a) SDS gel electrophoresis and isoelectric focusing both separate native proteins
(b) SDS gel electrophoresis and isoelectric focusing both make use of the migration of proteins in an electric field
(c) SDS gel electrophoresis and isoelectric focusing both require a ph gradient
(d) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
✅ Correct answer: (D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Explanation:
2D gel electrophoresis (Two-Dimensional Gel Electrophoresis) is a technique that combines Isoelectric Focusing (IEF) and SDS-PAGE to separate proteins based on two properties: charge and size.
Steps in 2D Gel Electrophoresis:
-
First dimension: Isoelectric Focusing (IEF)
- Proteins are separated based on their isoelectric point (pI) in a pH gradient.
- Proteins migrate in an electric field until they reach their pI, where they have no net charge and stop moving.
-
Second dimension: SDS-PAGE
- The separated proteins from IEF are then treated with SDS, which gives them a uniform negative charge.
- They are separated based on molecular weight (size) in the polyacrylamide gel.
- Smaller proteins migrate farther, and larger proteins move slower.
Together, these two techniques (IEF and SDS-PAGE) form 2D gel electrophoresis, allowing high-resolution protein separation.
Why Not the Other Options?
-
(A) SDS gel electrophoresis and isoelectric focusing both separate native proteins ❌
- Incorrect, because SDS-PAGE denatures proteins, while IEF can be done under denaturing or native conditions.
- In 2D-PAGE, SDS-PAGE is used after IEF, so the final proteins are denatured, not native.
-
(B) SDS gel electrophoresis and isoelectric focusing both make use of the migration of proteins in an electric field ❌
- Partially true, but incomplete.
- Both techniques involve electrophoresis (movement in an electric field), but they separate proteins based on different principles (charge in IEF, size in SDS-PAGE).
-
(C) SDS gel electrophoresis and isoelectric focusing both require a pH gradient ❌
- Incorrect, because only IEF requires a pH gradient.
- SDS-PAGE does not use a pH gradient; it separates proteins based on size using SDS.
Final Answer:
✅ (D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis



108 Comments
Parul
February 25, 2025Thankyou sir. I got confused between two answers but through solutions you provided I easily understood which one is correct.
Mahima Sharma
August 24, 2025Firstly ief and 2nd sds together make up the process of 2d gel
Tamanna Fogat
February 25, 2025Done ✅
Nisha
February 25, 2025Done
Priyam choudhary
February 25, 2025Oh sir
Shivani meena
February 26, 2025Done ✅
Prami masih
February 25, 2025Very nice sir 👍
Prami masih
February 25, 2025Well done 👍✅
Rohit Meena
February 25, 2025High resolution bhi hota Hai sir
Amit
February 25, 2025Best
Ujjwal jangir
February 25, 2025Nice
Ujjwal jangir
February 25, 2025Good
Shreeji Charan
February 26, 2025My answer was wright for this question
Arvind
February 26, 2025👍
Lavanya Sharma
February 26, 2025👍🏻
Suman bhakar
February 26, 2025Thanks sir
Ashwini Shekhawat
February 26, 2025👍👍
Beena meena
February 26, 2025👍
Lokesh kumawat
February 26, 2025Done 🤙
Abhilasha
February 26, 2025Done👍🏻
Hemlata Meena
February 26, 2025Correct
Hemlata Meena
February 26, 2025Done sir
Beena
February 26, 2025👍
Arushi saini
February 26, 2025👍
Arushi saini
February 26, 2025👌
Arushi saini
February 26, 2025☑️
Pooja jat
February 26, 2025Nice explanation .
Vikram Gahlot
February 26, 2025👍
Vikram Gahlot
February 26, 2025👍👍
Vikram Gahlot
February 26, 2025👍 ok
Vikram
February 26, 2025Nice sir
Jyoti
February 27, 2025Done ✅
Kabeer Narwal
February 27, 2025👍
yogesh sharma
February 27, 2025Great explanation sir 🙏
Pallavi gautam
February 27, 2025Ok sir
Sakshi
August 25, 2025sds gel electrophoresis and 2-d gel electrophoresis together make 2 -dimensional gel
yogesh sharma
February 27, 2025Done sir
Rohit Meena
February 28, 2025Yes 💯
Priya khandal
March 2, 2025Done sir
Sumit Sultan
March 3, 2025🙌👏👍
Anita Choudhary
April 18, 2025Done
Mohd juber Ali
August 14, 2025Option (D)
Anurag Giri
August 14, 2025Ans d in 2d-gel 1st -ief
2nd- sds gel
Khushi Agarwal
August 14, 2025Option d is Right
Dharmpal Swami
August 14, 2025IEF and SDS make 2d gel electrophoresis
Priya dhakad
August 14, 20252D gel electrophoresis is a technique that combines IEF nd SDS-PAGE to separate proteins based on charge and size.
Savita Garwa
August 14, 2025Done done
Anjani sharma
August 14, 2025Answer d is correct as in 2D gel electrophoresis the proteins are separated on the basis of charge and size
Akshita Saini
August 14, 2025Correct answer( D)
SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Soniya Shekhawat
August 14, 20254th is correct option.
Sneha Kumawat
August 14, 2025The correct answer is D
Mansukh Kapoor
August 14, 2025The correct answer is option 4th because SDS Page and IEF both makes the process of the 2D gel electrophoresis
Aafreen
August 17, 2025Ans- D
2D gel electrophoresis is a technique that combines IEF and SDS-PAGE to separate proteins based on two properties:- charge and size.
Nilofar Khan
August 14, 2025Correct answer is d
Karishma Don
August 14, 20252D gel- 1st is IEF and 2nd SDS GEL ELECTROPHORESIS
Khushi Mehra
August 14, 2025Answer is d
Isoelectric focussing and SDS page gel electrophoresis together form the procedure of 2D gel electrophoresis
shruti sharma
August 14, 2025option d is correct
Dipti Sharma
August 14, 2025(D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Meenakshi Choudhary
August 14, 2025Sds gel electrophoresis and ief together make up process of 2d gel electrophoresis
Simran saini
August 14, 2025option d is correct answer
Simran saini
August 14, 2025Option d is correct.
Neelam Sharma
August 14, 2025SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Deepika Sheoran
August 14, 2025Option d is correct answer
Because SDS gel & isoelectric focusing both make up the process of 2D Gel electrophoresis.
Priti khandal
August 14, 2025IEF and sds dono 2 d ko banate h
Komal Pareek
August 14, 2025d is correct
Priya khandal
August 14, 2025Sts gel electrophoresis and isoelectric focusing together makeup the process of 2D gel
Roopal Sharma
August 14, 20252D gel electrophoresis seperate proteins on the basis of both charge and maas
Mitali
August 15, 2025(D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Shivani
August 15, 2025Option d is correct
Divya rani
August 16, 2025Yes both SDS and IEF together is known as 2D gel electrophoresis.
Seema
August 18, 2025D)
Kavita choudhary
August 17, 2025Sds gel electrophoresis and isoelectric focusing both together make up 2D gel
Kajal
August 17, 2025Sds gel electrophoresis and isoelectric focusing both together make up 2 D gel
Khushi Vaishnav
August 17, 2025SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Varsha tatla
August 19, 2025Nicely cleared
Avni
August 17, 2025d) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Simran Saini
August 18, 2025Correct answer d.
SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis.
Neeraj Sharma
August 18, 2025Option D is correct as 2D gel electrophoresis includes IEF in 1D and SDS PAGE in 2nd D.
Manisha choudhary
August 20, 2025Sds denaturant h isliye sds page m native form nhi rahti protein ki jabki isoelectric Focusing m rahti h
Sds page and iso electric focusing dono m protein electromobaility, mtlb electric field m move krti h pr different principle p iEF m pi value k base p jaha perticular ph p charge zero hota h and sds page
m size k base p
Sds page m ph gradient nhi hota isoelectric focusing m ph gradient hota h
Sds page and isoelectric Focusing dono milkr 2D gel bnate h
Komal Sharma
August 21, 20252D gel electrophoresis two-Dimensional Gel Electrophoresis is a technique that combines Isoelectric Focusing (IEF) and SDS-PAGE to separate proteins based on two properties: charge and size so option d is correct
Akshita Jangid
August 24, 2025Both SDS and IEF together make 2D gel electrophoresis
Bharti Yadav
August 24, 2025SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Priyanka Choudhary
August 24, 2025Right answer is option d because of this
2D gel electrophoresis is a technique that combines Isoelectric Focusing and SDS-PAGE to separate proteins based on two properties: charge and size
Urmi sisodiya
August 24, 2025Answer is d
Because 2d gel is the combination of SDS page and isoelectric focusing.
It does not depend on ph gradient but only isoelectric focusing depends on ph gradient.
Priyanka Choudhary
August 24, 2025D is correct answer,
SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Bhavana kankhedia
August 24, 2025SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Ankita Pareek
August 24, 2025Sds gel electrophoresis and isoelectric focusing together makes 2d gel electrophoresis
Bhavana kankhedia
August 24, 20252D gel electrophoresis includes IEF in 1D and SDS PAGE in 2nd D.
Sonam Saini
August 24, 2025SDS gel electroconduction and isoelectric focusing together form 2D electroconducting gels
Niti Tanwar
August 24, 2025D is right
SDS gel electrophoresis and isoelectric focusing together make 2d gel electrophoresis
Falguni Prakash
August 24, 2025D
Kashish yadav
August 25, 2025Ok sir
sakshi khatri
August 25, 2025done
Anju
August 25, 2025Ans: D
Ayush Dubey
August 25, 2025SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Devika
August 25, 2025Sds gel electrophoresis and isoelectric point together make 2Dgel
shruti sharma
August 25, 2025both SDS and IEF together is known as 2D gel electrophoresis.
Pratibha Jain
August 30, 2025Correct answer is option (D)
SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Sheikh Aleen
August 30, 2025SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Anisha Beniwal
August 31, 2025SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Vanshika Sharma
August 31, 2025Sds gel and isoelectric focusing together make up the process of 2D gel electrophoresis
Asha Gurzzar
September 1, 2025Option d is correct ,sds gel and isoelectric focusing together makeup the process of 2D gel electrophoresis
Ajay Sharma
September 2, 2025Sds on the basis of mw and isoelectric focusing on ph gradient
Anisha jakhar
September 3, 2025(D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis
Muskan Yadav
September 3, 2025SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis.
Somya Yadav
September 5, 2025SDS gel electrophoresis and IFF together make 2D gel electrophoresis
Meera Gurjar
September 13, 2025Both make 2D gel electrophoresis
Aakansha sharma Sharma
October 1, 2025Correct answer: (D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis