2D gel electrophoresis Isoelectric Focusing (IEF) and SDS-PAGE

Which of the following statement about 2D gel electrophoresis is correct?

(a) SDS gel electrophoresis and isoelectric focusing both separate native proteins
(b) SDS gel electrophoresis and isoelectric focusing both make use of the migration of proteins in an electric field
(c) SDS gel electrophoresis and isoelectric focusing both require a ph gradient
(d) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

Correct answer: (D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis


Explanation:

2D gel electrophoresis (Two-Dimensional Gel Electrophoresis) is a technique that combines Isoelectric Focusing (IEF) and SDS-PAGE to separate proteins based on two properties: charge and size.

Steps in 2D Gel Electrophoresis:

  1. First dimension: Isoelectric Focusing (IEF)

    • Proteins are separated based on their isoelectric point (pI) in a pH gradient.
    • Proteins migrate in an electric field until they reach their pI, where they have no net charge and stop moving.
  2. Second dimension: SDS-PAGE

    • The separated proteins from IEF are then treated with SDS, which gives them a uniform negative charge.
    • They are separated based on molecular weight (size) in the polyacrylamide gel.
    • Smaller proteins migrate farther, and larger proteins move slower.

Together, these two techniques (IEF and SDS-PAGE) form 2D gel electrophoresis, allowing high-resolution protein separation.


Why Not the Other Options?

  • (A) SDS gel electrophoresis and isoelectric focusing both separate native proteins

    • Incorrect, because SDS-PAGE denatures proteins, while IEF can be done under denaturing or native conditions.
    • In 2D-PAGE, SDS-PAGE is used after IEF, so the final proteins are denatured, not native.
  • (B) SDS gel electrophoresis and isoelectric focusing both make use of the migration of proteins in an electric field

    • Partially true, but incomplete.
    • Both techniques involve electrophoresis (movement in an electric field), but they separate proteins based on different principles (charge in IEF, size in SDS-PAGE).
  • (C) SDS gel electrophoresis and isoelectric focusing both require a pH gradient

    • Incorrect, because only IEF requires a pH gradient.
    • SDS-PAGE does not use a pH gradient; it separates proteins based on size using SDS.

Final Answer:

(D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

108 Comments
  • Parul
    February 25, 2025

    Thankyou sir. I got confused between two answers but through solutions you provided I easily understood which one is correct.

    • Mahima Sharma
      August 24, 2025

      Firstly ief and 2nd sds together make up the process of 2d gel

  • Tamanna Fogat
    February 25, 2025

    Done ✅

  • Nisha
    February 25, 2025

    Done

  • Priyam choudhary
    February 25, 2025

    Oh sir

    • Shivani meena
      February 26, 2025

      Done ✅

  • Prami masih
    February 25, 2025

    Very nice sir 👍

  • Prami masih
    February 25, 2025

    Well done 👍✅

  • Rohit Meena
    February 25, 2025

    High resolution bhi hota Hai sir

  • Amit
    February 25, 2025

    Best

  • Ujjwal jangir
    February 25, 2025

    Nice

  • Ujjwal jangir
    February 25, 2025

    Good

  • Shreeji Charan
    February 26, 2025

    My answer was wright for this question

  • Arvind
    February 26, 2025

    👍

  • Lavanya Sharma
    February 26, 2025

    👍🏻

  • Suman bhakar
    February 26, 2025

    Thanks sir

  • Ashwini Shekhawat
    February 26, 2025

    👍👍

    • Beena meena
      February 26, 2025

      👍

  • Lokesh kumawat
    February 26, 2025

    Done 🤙

  • Abhilasha
    February 26, 2025

    Done👍🏻

  • Hemlata Meena
    February 26, 2025

    Correct

  • Hemlata Meena
    February 26, 2025

    Done sir

  • Beena
    February 26, 2025

    👍

  • Arushi saini
    February 26, 2025

    👍

  • Arushi saini
    February 26, 2025

    👌

  • Arushi saini
    February 26, 2025

    ☑️

  • Pooja jat
    February 26, 2025

    Nice explanation .

  • Vikram Gahlot
    February 26, 2025

    👍

  • Vikram Gahlot
    February 26, 2025

    👍👍

  • Vikram Gahlot
    February 26, 2025

    👍 ok

  • Vikram
    February 26, 2025

    Nice sir

  • Jyoti
    February 27, 2025

    Done ✅

  • Kabeer Narwal
    February 27, 2025

    👍

  • yogesh sharma
    February 27, 2025

    Great explanation sir 🙏

  • Pallavi gautam
    February 27, 2025

    Ok sir

    • Sakshi
      August 25, 2025

      sds gel electrophoresis and 2-d gel electrophoresis together make 2 -dimensional gel

  • yogesh sharma
    February 27, 2025

    Done sir

  • Rohit Meena
    February 28, 2025

    Yes 💯

  • Priya khandal
    March 2, 2025

    Done sir

  • Sumit Sultan
    March 3, 2025

    🙌👏👍

  • Anita Choudhary
    April 18, 2025

    Done

  • Mohd juber Ali
    August 14, 2025

    Option (D)

  • Anurag Giri
    August 14, 2025

    Ans d in 2d-gel 1st -ief
    2nd- sds gel

  • Khushi Agarwal
    August 14, 2025

    Option d is Right

  • Dharmpal Swami
    August 14, 2025

    IEF and SDS make 2d gel electrophoresis

  • Priya dhakad
    August 14, 2025

    2D gel electrophoresis is a technique that combines IEF nd SDS-PAGE to separate proteins based on charge and size.

  • Savita Garwa
    August 14, 2025

    Done done

  • Anjani sharma
    August 14, 2025

    Answer d is correct as in 2D gel electrophoresis the proteins are separated on the basis of charge and size

  • Akshita Saini
    August 14, 2025

    Correct answer( D)
    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Soniya Shekhawat
    August 14, 2025

    4th is correct option.

  • Sneha Kumawat
    August 14, 2025

    The correct answer is D

  • Mansukh Kapoor
    August 14, 2025

    The correct answer is option 4th because SDS Page and IEF both makes the process of the 2D gel electrophoresis

    • Aafreen
      August 17, 2025

      Ans- D
      2D gel electrophoresis is a technique that combines IEF and SDS-PAGE to separate proteins based on two properties:- charge and size.

  • Nilofar Khan
    August 14, 2025

    Correct answer is d

  • Karishma Don
    August 14, 2025

    2D gel- 1st is IEF and 2nd SDS GEL ELECTROPHORESIS

  • Khushi Mehra
    August 14, 2025

    Answer is d
    Isoelectric focussing and SDS page gel electrophoresis together form the procedure of 2D gel electrophoresis

  • shruti sharma
    August 14, 2025

    option d is correct

  • Dipti Sharma
    August 14, 2025

    (D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Meenakshi Choudhary
    August 14, 2025

    Sds gel electrophoresis and ief together make up process of 2d gel electrophoresis

  • Simran saini
    August 14, 2025

    option d is correct answer

  • Simran saini
    August 14, 2025

    Option d is correct.

  • Neelam Sharma
    August 14, 2025

    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Deepika Sheoran
    August 14, 2025

    Option d is correct answer
    Because SDS gel & isoelectric focusing both make up the process of 2D Gel electrophoresis.

  • Priti khandal
    August 14, 2025

    IEF and sds dono 2 d ko banate h

  • Komal Pareek
    August 14, 2025

    d is correct

  • Priya khandal
    August 14, 2025

    Sts gel electrophoresis and isoelectric focusing together makeup the process of 2D gel

  • Roopal Sharma
    August 14, 2025

    2D gel electrophoresis seperate proteins on the basis of both charge and maas

  • Mitali
    August 15, 2025

    (D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Shivani
    August 15, 2025

    Option d is correct

  • Divya rani
    August 16, 2025

    Yes both SDS and IEF together is known as 2D gel electrophoresis.

    • Seema
      August 18, 2025

      D)

  • Kavita choudhary
    August 17, 2025

    Sds gel electrophoresis and isoelectric focusing both together make up 2D gel

  • Kajal
    August 17, 2025

    Sds gel electrophoresis and isoelectric focusing both together make up 2 D gel

  • Khushi Vaishnav
    August 17, 2025

    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

    • Varsha tatla
      August 19, 2025

      Nicely cleared

  • Avni
    August 17, 2025

    d) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Simran Saini
    August 18, 2025

    Correct answer d.
    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis.

  • Neeraj Sharma
    August 18, 2025

    Option D is correct as 2D gel electrophoresis includes IEF in 1D and SDS PAGE in 2nd D.

  • Manisha choudhary
    August 20, 2025

    Sds denaturant h isliye sds page m native form nhi rahti protein ki jabki isoelectric Focusing m rahti h
    Sds page and iso electric focusing dono m protein electromobaility, mtlb electric field m move krti h pr different principle p iEF m pi value k base p jaha perticular ph p charge zero hota h and sds page
    m size k base p
    Sds page m ph gradient nhi hota isoelectric focusing m ph gradient hota h
    Sds page and isoelectric Focusing dono milkr 2D gel bnate h

  • Komal Sharma
    August 21, 2025

    2D gel electrophoresis two-Dimensional Gel Electrophoresis is a technique that combines Isoelectric Focusing (IEF) and SDS-PAGE to separate proteins based on two properties: charge and size so option d is correct

  • Akshita Jangid
    August 24, 2025

    Both SDS and IEF together make 2D gel electrophoresis

  • Bharti Yadav
    August 24, 2025

    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Priyanka Choudhary
    August 24, 2025

    Right answer is option d because of this
    2D gel electrophoresis is a technique that combines Isoelectric Focusing and SDS-PAGE to separate proteins based on two properties: charge and size

  • Urmi sisodiya
    August 24, 2025

    Answer is d
    Because 2d gel is the combination of SDS page and isoelectric focusing.
    It does not depend on ph gradient but only isoelectric focusing depends on ph gradient.

  • Priyanka Choudhary
    August 24, 2025

    D is correct answer,
    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Bhavana kankhedia
    August 24, 2025

    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Ankita Pareek
    August 24, 2025

    Sds gel electrophoresis and isoelectric focusing together makes 2d gel electrophoresis

  • Bhavana kankhedia
    August 24, 2025

    2D gel electrophoresis includes IEF in 1D and SDS PAGE in 2nd D.

  • Sonam Saini
    August 24, 2025

    SDS gel electroconduction and isoelectric focusing together form 2D electroconducting gels

  • Niti Tanwar
    August 24, 2025

    D is right
    SDS gel electrophoresis and isoelectric focusing together make 2d gel electrophoresis

  • Falguni Prakash
    August 24, 2025

    D

  • Kashish yadav
    August 25, 2025

    Ok sir

  • sakshi khatri
    August 25, 2025

    done

  • Anju
    August 25, 2025

    Ans: D

  • Ayush Dubey
    August 25, 2025

    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Devika
    August 25, 2025

    Sds gel electrophoresis and isoelectric point together make 2Dgel

  • shruti sharma
    August 25, 2025

    both SDS and IEF together is known as 2D gel electrophoresis.

  • Pratibha Jain
    August 30, 2025

    Correct answer is option (D)
    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Sheikh Aleen
    August 30, 2025

    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Anisha Beniwal
    August 31, 2025

    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Vanshika Sharma
    August 31, 2025

    Sds gel and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Asha Gurzzar
    September 1, 2025

    Option d is correct ,sds gel and isoelectric focusing together makeup the process of 2D gel electrophoresis

  • Ajay Sharma
    September 2, 2025

    Sds on the basis of mw and isoelectric focusing on ph gradient

  • Anisha jakhar
    September 3, 2025

    (D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

  • Muskan Yadav
    September 3, 2025

    SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis.

  • Somya Yadav
    September 5, 2025

    SDS gel electrophoresis and IFF together make 2D gel electrophoresis

  • Meera Gurjar
    September 13, 2025

    Both make 2D gel electrophoresis

  • Aakansha sharma Sharma
    October 1, 2025

    Correct answer: (D) SDS gel electrophoresis and isoelectric focusing together make up the process of 2D gel electrophoresis

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