The tracking dye used in SDS PAGE will be
(a) Anionic
(b) Cationic
(c) Non ionic
(d) Amphipathic
The correct answer is:
(a) Anionic
Explanation:
In SDS-PAGE, tracking dyes like Bromophenol Blue, Xylene Cyanol, and Orange G are used to monitor the movement of proteins through the gel. These dyes are anionic (negatively charged) at the alkaline pH of the running buffer (typically pH ~8.3).
- Anionic nature allows the dye to migrate towards the positive electrode (anode), just like the proteins in the gel.
- This migration helps visualize the electrophoresis progress and prevents running the gel for too long.
Why not the other options?
- (b) Cationic → Cationic dyes would migrate in the opposite direction, which is not useful in SDS-PAGE.
- (c) Non-ionic → A non-ionic dye wouldn’t migrate with the proteins, making it ineffective as a tracking dye.
- (d) Amphipathic → Tracking dyes do not need both hydrophilic and hydrophobic regions, so they are not amphipathic.
Thus, the correct answer is (a) Anionic ✅
76 Comments
Suman bhakar
February 26, 2025Was correct
Mahima Sharma
August 24, 2025Anionic
Arushi
February 26, 2025☑️☑️
Parul
February 27, 2025Question is easily understood. Done
Pooja jat
February 27, 2025Done.
Lavanya Sharma
February 27, 2025Solving these questions is like a quick revision of the topics.
Prami Masih
February 27, 2025Okay sir
Akshay mahawar
February 27, 2025Done👍
yogesh sharma
February 27, 2025Done sir
Lokesh kumawat
March 1, 2025Done
Abhilasha
March 1, 2025Was right
Nisha
March 1, 2025Best variety of learning formats
VIKRAM GAHLOT
March 7, 2025👍
Anurag Giri
August 13, 2025Ans 1 Anionic nature allows the dye to migrate towards the positive electrode (anode), just like the proteins in the gel
Aman Choudhary
August 13, 2025Anionic dye is used in gel because it provide negative charge by which the sample travel towards positive electrode
Khushi Agarwal
August 14, 2025Anionic detergent
Dharmpal Swami
August 14, 2025Anionic
Anjani sharma
August 14, 2025Anionic answer 1 sds provide negative charge on the protein
Neelam Sharma
August 14, 2025Anionic
Karishma Don
August 14, 2025anionic as the bromophenol and other dyes can migrate towards the positive electrode
Mitali
August 14, 2025the correct answer is (a) Anionic ✅
Soniya Shekhawat
August 14, 2025In SDS page in which tracking dye is bromo phenol blue which is an anionic .
Nilofar Khan
August 14, 2025Correct answer is a anionic
Roopal Sharma
August 14, 2025Anionic dye is used in SDS
Priya dhakad
August 14, 2025The correct answer is anionic because BPB dye is anionic in SDS Page
Mansukh Kapoor
August 14, 2025The correct answer is option 1st
The tracking dye should be anionic because due to this property they could travel towards the anode in SDS Page
Deepika Sheoran
August 14, 2025Anionic SDS provide negative charge on the protein.
Mohd juber Ali
August 14, 2025Option a is right
Anion= BFB(bromo phenol blue)
-ve charge move toward anode
M.w. ⬇️ ( fast move )
Dipti Sharma
August 14, 2025Correct answer is 1
Bromophenol Blue, Xylene Cyanol, and Orange G are anionic in nature. Therefore ,migrate towards positive charge
Sneha Kumawat
August 14, 2025Anoionic treaking dyes used in sds
Priyanka choudhary
August 14, 2025Correct answer is anionic
Monika jangid
August 14, 2025Tracking dye is bpb which is anionic in nature so -ve charge move towards positive or anode
Surbhi Rajawat
August 14, 2025Dyes used in SDS are anionic so as to facilitate their movement towards positive electrode. Just like the protein
Shivani
August 14, 2025(a) Anionic.
Tracking dye in sds page will be Anionic migrates towards positive charge
Priya khandal
August 14, 2025Negative
Ankita Pareek
August 15, 2025The tracking dye in sds will be anionic
Kajal
August 15, 2025Option a
Anionic
Kavita choudhary
August 17, 2025Anionic
Aafreen
August 17, 2025Anionic is correct answer
Anionic are negatively charge and and migrates towards positive charge electrode
Avni
August 17, 2025anionic is the correct answer
Neeraj Sharma
August 17, 2025Option A i.e. Anionic is correct as the dye is negatively charged which helps the dye to move faster in the gel
Simran Saini
August 18, 2025Option A.
Anionic
Ankita Pareek
August 18, 2025Tracking dyes are anionic in sds PAGE
Varsha Tatla
August 23, 2025Anionic
shruti sharma
August 25, 2025anionic as the bromophenol and other dyes can migrate towards the positive electrode
Bharti Yadav
August 24, 2025Tracking dye are Anionic in nature in SDS -PAGE
Priyanka Choudhary
August 24, 2025Sds page will be Anionic
MOHIT AKHAND
August 24, 2025Option A
Done sir ✅
Urmi sisodiya
August 24, 2025In SDS the gel is used is anionic in nature so it provides negative charge on the protein molecules
Anil Choudhary
August 24, 2025Anionic
Niti Tanwar
August 24, 2025Option a anionic
Alec
August 24, 2025option a because tracking dye is anionic in nature.
Sonam Saini
August 24, 2025एसडीएस में ट्रैकिंग दी नेगेटिव होगा
Anjali
August 24, 2025the correct answer is (a) Anionic
Anju
August 24, 2025Ans:a
Seema
August 24, 2025Anionic ,anionic behaviour helps the dye to migrate towards positive charged electrode
Aartii sharma
August 24, 2025Anionic
Shivani Panwar
August 24, 2025Answer is A
Devika
August 24, 2025Anionic
Falguni Prakash
August 24, 2025A
Kashish yadav
August 25, 2025Anionic
Ayush Dubey
August 25, 2025Anionic
sakshi khatri
August 25, 2025done
Pratibha Jain
August 30, 2025The correct answer is option (a) Anionic
Shriyanshi verma
August 30, 2025Question is easily understood. Done
Sheikh Aleen
August 30, 2025Correct ans is Anionic
Surendra Doodi
August 30, 2025Option A is correct………Anionic nature allows the dye to migrate towards the positive electrode (anode), just like the proteins in the gel.
Patel Radha
August 30, 2025Anionic
Komal Sharma
August 31, 2025In SDS-PAGE, tracking dyes like Bromophenol Blue, Xylene Cyanol, and Orange G are used to monitor the movement of proteins through the gel. These dyes are anionic (negatively charged) at the alkaline pH of the running buffer (typically pH ~8.3).
Vanshika Sharma
August 31, 2025In sds page tracking dues used to monitor the movement of protiens through the gel these dues are anionic
Shobha Kanwar
August 31, 2025Anionic answer a) bcoz sds provide negative charge on the protein
Anisha jakhar
September 3, 2025Anionic
Muskan Yadav
September 3, 2025Anionic nature allows the dye to migrate towards the positive electrode (anode), just like the proteins in the gel.
Somya Yadav
September 5, 2025Anionic
Ajay Sharma
September 6, 2025Tracking dye should be anionic so can help in tracking the movement of protein and gel procedure
Aakansha sharma Sharma
October 1, 2025Anionic