Tracking Dye in SDS PAGE of Protein

Which of these is used as a tracking dye in SDS PAGE of protein
(a) Bromo phenol blue
(b) Xylene cyanol
(c) Orange G
(d) All of the above

 

The correct answer is:

(d) All of the above

Explanation:

In SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), tracking dyes are used to monitor the progress of electrophoresis and ensure proper migration of proteins. Different dyes migrate at different speeds, allowing flexibility based on experimental needs.

  1. Bromophenol Blue (BPB)Most commonly used tracking dye. It migrates faster than most proteins.
  2. Xylene Cyanol → Moves slower than bromophenol blue and is useful for tracking larger proteins.
  3. Orange G → Moves faster than bromophenol blue and is sometimes used when tracking smaller peptides or when alternative dyes are needed.

Since all three dyes can be used as tracking dyes in SDS-PAGE, the correct answer is:
(d) All of the above

76 Comments
  • Suman bhakar
    February 26, 2025

    Done

    • Mahima Sharma
      August 24, 2025

      Bpb, xylene cyanol, orang g

  • Arushi
    February 26, 2025

    ☑️👍

  • Parul
    February 27, 2025

    Done sir

  • Pooja jat
    February 27, 2025

    Easily understood the question…,,, done!

  • Lavanya Sharma
    February 27, 2025

    Got it sir!

  • Lavanya Sharma
    February 27, 2025

    ✅👍🏻

  • Prami Masih
    February 27, 2025

    Awesome sir

  • Akshay mahawar
    February 27, 2025

    Done 👍

  • yogesh sharma
    February 27, 2025

    Easily explained sir

  • Lokesh kumawat
    March 1, 2025

    Done

  • Abhilasha
    March 1, 2025

    Done

  • Nisha
    March 1, 2025

    Done

  • VIKRAM GAHLOT
    March 7, 2025

    👍

  • Anita choudhary
    April 19, 2025

    Done👍

  • Anurag Giri
    August 13, 2025

    Ans 4 all are correct

  • Aman Choudhary
    August 13, 2025

    BPB, Xylene Cyanol and orange G is used

  • Khushi Agarwal
    August 14, 2025

    All of above

  • Dharmpal Swami
    August 14, 2025

    All of these are use for SDS page

  • Neelam Sharma
    August 14, 2025

    All of these

  • Anjani sharma
    August 14, 2025

    All of these are used as tracking dye

  • Karishma Don
    August 14, 2025

    all the above dyes, as they are anionic

  • Mitali
    August 14, 2025

    all three dyes can be used as tracking dyes in SDS-PAGE, the correct answer is:
    (d) All of the above ✅

  • Soniya Shekhawat
    August 14, 2025

    All of the above.

  • Roopal Sharma
    August 14, 2025

    Bromophenol blue ,orange g, xylene cynol all are used for tracking

  • Priya dhakad
    August 14, 2025

    Answer is d
    All three dyes are used as tracking dye in SDS PHAGE

  • Mansukh Kapoor
    August 14, 2025

    The correct answer is option 4th
    All the dyes are used in tracking of the sample in gel

  • Mohd juber Ali
    August 14, 2025

    Option d is the right answer

  • Deepika Sheoran
    August 14, 2025

    Option 4th is correct answer
    Because all three dyes can be used as tracking dyes in SDS PAGE.

  • Dipti Sharma
    August 14, 2025

    (d) All of the above

  • HIMANI FAUJDAR
    August 14, 2025

    All of these are used as tracking dye

  • Sneha Kumawat
    August 14, 2025

    All of the above

  • Surbhi Rajawat
    August 14, 2025

    Understood.

  • Priyanka choudhary
    August 14, 2025

    All off above

  • Shivani
    August 14, 2025

    All of the above .

  • Priya khandal
    August 14, 2025

    All of the above

  • Kajal
    August 15, 2025

    All of these

  • shruti sharma
    August 16, 2025

    all of these

  • Kavita choudhary
    August 17, 2025

    All of the above

  • Aafreen
    August 17, 2025

    Ans – 4 All of the Above

  • Avni
    August 17, 2025

    All of the above

  • Neeraj Sharma
    August 17, 2025

    Option D is correct

  • Simran Saini
    August 18, 2025

    All of the above.

  • Varsha Tatla
    August 23, 2025

    Bromo phenol , orange g etc all of these

  • Bharti Yadav
    August 24, 2025

    Bromophenol blue, xylene cyanol,orange G all three dyes can be used as tracking dyes in SDS -PAGE

  • Priyanka Choudhary
    August 24, 2025

    Sds page of protein,
    Bromo phenol blue,Xylene cyanol, Orange G

  • MOHIT AKHAND
    August 24, 2025

    D
    Done sir ✅

  • Urmi sisodiya
    August 24, 2025

    All of the above

  • Anil Choudhary
    August 24, 2025

    All

  • Bhavana kankhedia
    August 24, 2025

    All of the above

  • Alec
    August 24, 2025

    all of the above dyes are used.

  • Sonam Saini
    August 24, 2025

    Option d is right answer

  • Niti Tanwar
    August 24, 2025

    All of the above

  • Anjali
    August 24, 2025

    All of the above

  • Anju
    August 24, 2025

    All of the above

  • Seema
    August 24, 2025

    D) all of these dyes are used.

  • Shivani Panwar
    August 24, 2025

    All of the above….

  • Devika
    August 24, 2025

    All of these

  • Falguni Prakash
    August 24, 2025

    All of them

  • Kashish yadav
    August 25, 2025

    Ok sir 👍

  • Ayush Dubey
    August 25, 2025

    All of the above

  • sakshi khatri
    August 25, 2025

    done

  • Pratibha Jain
    August 30, 2025

    The correct answer is option (d)
    All of the above

  • Shriyanshi verma
    August 30, 2025

    Bpb, xylene cyanol, orang g

  • Surendra Doodi
    August 30, 2025

    Bromophenol Blue ,Xylene Cyanol, orange G all

  • Patel Radha
    August 30, 2025

    Done ✔️

  • Komal Sharma
    August 31, 2025

    In SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), tracking dyes are used to monitor the progress of electrophoresis and ensure proper migration of proteins. Different dyes migrate at different speeds, allowing flexibility based on experimental needs.

  • Vanshika Sharma
    August 31, 2025

    All of the above dyes used in SDS

  • Shobha Kanwar
    August 31, 2025

    All of these :Bromophenol blue ,orange g, xylene cynol all are used for tracking

  • Aartii sharma
    September 1, 2025

    All of these are used as dye

  • Anisha jakhar
    September 3, 2025

    The correct option is (d) All of the above

  • Muskan Yadav
    September 3, 2025

    All of these are used as dye ( bromophenol blue , xylene cyanol and orange g )

  • Somya Yadav
    September 5, 2025

    All of the above

  • Ajay Sharma
    September 6, 2025

    All of the above

  • Meera Gurjar
    September 13, 2025

    All of them

  • Aakansha sharma Sharma
    October 1, 2025

    Bromophenol blue ,orange g, xylene cynol all are used for tracking

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