(SEPT 2022-11) 72. An enzyme has a Km of 5 x 10-5 M and a Vmax of 100 µmoles.lit-1. min-1(Km is the Michaelis constant and Vmax is the maximal velocity). […]
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CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Demystifying Competitive Enzyme Inhibition: Substrate Competition and Reversibility
(DEC 2013 GU) 69. If the product of an enzyme binds to the enzyme- substrate complex to exhibit its activity through a decrease in both Km and Vmax, this type […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Analyzing Elastase Substrate Specificity Using Kinetic Data: Rate, Residue Size, and Cleavage Preferences
The correct answer is (2) (B), (D), (E). Introduction Elastase is a proteolytic enzyme known for its specificity based on the size and nature of amino acid residues adjacent to its […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Calculating Turnover Number (kcatkcat) for an Enzyme from Substrate and Product Data
(JUNE 2015) 40. A 1% (w/v) solution of a sugar polymer is digested by an enzyme (20µg, MW=200,000). The rate of monomer sugar (MW=400) liberated was determined to have a […]
![(DEC 2019) 37. Given below is the [P] vs time plot of an enzymatic reaction carried out by the enzyme X Which one of the following statements is the correct interpretation of the data? (1) The Km and Vmax of the enzyme X are 15 and 60 units, respectively. (2) The Vmax is 60 but the Km cannot be determined (3) The Km 15 but the Vmax cannot be determined (4) Neither the Km nor the Vmax of the enzyme X can be determined from these data](https://www.letstalkacademy.com/wp-content/uploads/2025/09/37.jpg)
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Interpreting [P] vs Time Plots in Enzyme Kinetics: Determining Vmax and Km
The correct answer is (2) The Vmax is 60 but the Km cannot be determined. Introduction Graphical data are fundamental tools in enzyme kinetics, helping derive quantitative parameters such as Vmax […]
![(SEPT 2022-ll) 35. Given below are plots of the linear derivation of Michaelis-Menten kinetic equation and statements related to the variables (initial velocity-Vo and substrate concentration - [S]) used. A. In plot (i), both x and y axes have dependent variables B. In plot (ii) neither x nor y axis has independent variables C. In plot (i) only y-axis has a dependent variable D. In both the plots, x axis has an independent variable](https://www.letstalkacademy.com/wp-content/uploads/2025/09/35.jpg)
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Identifying Dependent and Independent Variables in Enzyme Kinetic Linear Plots
The correct answer is (1) A, B and D only. Introduction Linear transformations of the Michaelis-Menten equation allow extraction of key enzyme kinetics parameters. These transforms produce specific plots, notably the […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Steady-State Hypothesis in Enzyme Kinetics: Explanation and Key Assumptions
(DEC 2006) 20. The steady state hypothesis for enzyme suggest that (1) Rate of formation of ES complex is equal to rate of breakdown of ES complex (2) Rate of […]
