Protein Separation Role of SDS Explained

By adding SDS (sodium dodecyl sulfate) during the electrophoresis of proteins, it is possible to:

(a) Determine a protein’s isoelectric point.
(b) Determine an enzyme’s specific activity.
(c) Determine the amino acid composition of the protein.
(d) Separate proteins exclusively on the basis of molecular weight.

The correct answer is:

(d) Separate proteins exclusively on the basis of molecular weight.

Explanation:

Sodium dodecyl sulfate (SDS) is an anionic detergent used in SDS-PAGE (SDS-polyacrylamide gel electrophoresis). It denatures proteins by disrupting non-covalent bonds and imparts a uniform negative charge to all proteins. This ensures that proteins migrate through the gel based solely on their molecular weight, rather than their shape or charge.

  • (a) Incorrect: Isoelectric focusing (IEF), not SDS-PAGE, is used to determine a protein’s isoelectric point (pI). SDS masks the protein’s charge, making pI determination impossible.
  • (b) Incorrect: Enzyme activity is not assessed through SDS-PAGE, as SDS denatures proteins, making them inactive.
  • (c) Incorrect: SDS-PAGE does not provide information about amino acid composition; techniques like mass spectrometry or amino acid analysis are used for that.

 


 

 

83 Comments
  • Pooja jat
    February 27, 2025

    Done.,, easy one.

  • Lavanya Sharma
    February 27, 2025

    ✅👍🏻

    • Mahima Sharma
      August 24, 2025

      D

  • Lavanya Sharma
    February 27, 2025

    😄👍🏻

  • Prami Masih
    February 27, 2025

    Good website

  • yogesh sharma
    February 27, 2025

    Done sir

  • Lokesh kumawat
    March 1, 2025

    Done

  • Abhilasha
    March 1, 2025

    Easy done👍🏻

  • Nisha
    March 1, 2025

    Well structured learning content

  • Suman bhakar
    March 3, 2025

    👍👍

    • Priyanka choudhary
      August 14, 2025

      Right answer is option d,sds separation by mw

  • VIKRAM GAHLOT
    March 7, 2025

    👍

  • Arushi
    April 1, 2025

    👍✔️

  • Anita choudhary
    April 19, 2025

    ☑️👍

  • Dharmpal Swami
    August 13, 2025

    Sds seprate protein based on their molecular weight

  • Deepika sheoran
    August 13, 2025

    Option d is correct answer
    Because saparate protein migrate the gel based on their molecular weight.

  • Deepika sheoran
    August 13, 2025

    Sds saprate protein migrate the gel based on their molecular weight.

  • Aman Choudhary
    August 13, 2025

    SDS denature the protein and give uniform negative charge to all proteins by which identified on the basis of molecular weight not on charge and shape

  • Neelam Sharma
    August 14, 2025

    Separate proteins exclusively on the basis of molecular weight.

  • Santosh Saini
    August 14, 2025

    Seperation of protein on the basis of mole. Weight

  • Anurag Giri
    August 14, 2025

    Sds provides uniform negative charge to all proteins. This ensures that proteins migrate through the gel based solely on their molecular weight, rather than their shape or charge

  • Anjani sharma
    August 14, 2025

    Answer 4 is correct as sds provide uniform negative charge on protein, further they are separated on the basis on molecular weight

  • Karishma Don
    August 14, 2025

    SDS denatures the protein and also give -ve charge so the protein migrates according to mol. weight only.

  • Khushi Agarwal
    August 14, 2025

    Option d is correct answer
    Sds seperates by mw

  • Khushi Singh
    August 14, 2025

    Done

  • Soniya Shekhawat
    August 14, 2025

    Easy sir😃

  • Urmi sisodiya
    August 14, 2025

    Option d is correct bcoz SDS seperate protein based on their molecular weight

  • Khushi Mehra
    August 14, 2025

    SDS produce uniform negative charge on protein and this protein get separated by the SDS PAGE technique

  • Avni
    August 14, 2025

    Correct option is d
    Separate proteins on the basis of molecular weight.

  • Nilofar Khan
    August 14, 2025

    Correct answer is d

  • Roopal Sharma
    August 14, 2025

    SDS separate protein on basis of molecular weight

  • Payal
    August 14, 2025

    Option D separate protein on the basis of molecular weight.

  • Priya dhakad
    August 14, 2025

    Option d is correct separation of protein on the basis of mol. Wt.

  • Deepika Sheoran
    August 14, 2025

    SDS saprate protein on the basis of molecular weight.

  • Mansukh Kapoor
    August 14, 2025

    The correct answer is option 4th
    SDS helps into separation on the basis of molecular weight

  • Monika jangid
    August 14, 2025

    Sds seprate protein on the basis on mw

  • Mohd juber Ali
    August 14, 2025

    Option (4)
    Sodium dodecyl sulphate use in SDS page it is protein denature detergent separate protein on the basis of mass

  • Dipti Sharma
    August 14, 2025

    (d) Separate proteins exclusively on the basis of molecular weight

  • Surbhi Rajawat
    August 14, 2025

    SDS treatment is basically given to impart negative charge on protein so that we can run it on PAGE and separate it on the basis of molecular weight

  • Sneha Kumawat
    August 14, 2025

    Correct answer is for sds separate proteins by molecular weight

  • Shivani
    August 14, 2025

    Option d is correct.
    After adding sds it is possible to seprate protein exclusively on the basis of molecular weight

  • Priya khandal
    August 14, 2025

    Option d is right molecular weight ke according

  • Ankita Pareek
    August 15, 2025

    By sds page proteins separate on the basis of their molecular weight

  • Kajal
    August 15, 2025

    Separate protein exclusively acc. To their molecular weight

  • Kavita choudhary
    August 15, 2025

    Answer is d

  • shruti sharma
    August 16, 2025

    option d

  • Aafreen
    August 17, 2025

    Separate proteins exclusively on the basis of molecular weight

  • Neeraj Sharma
    August 17, 2025

    SDS will provide negative charge on the proteins hence it will help in separation of proteins on the basis of molecular weight

  • Simran Saini
    August 18, 2025

    Option d.
    Separate proteins exclusively on the basis of molecular weight.

  • Seema
    August 18, 2025

    D

  • Varsha Tatla
    August 23, 2025

    D option will be correct

  • Shrddha Modi
    August 23, 2025

    It’s just two determine sample on the basis of molecular weight OP. D

  • Bharti Yadav
    August 24, 2025

    Separate proteins exclusively on the basis of molecular weight.

  • Priyanka Choudhary
    August 24, 2025

    Answer d is correct answer,Separate proteins exclusively on the basis of molecular weight

  • MOHIT AKHAND
    August 24, 2025

    Done sir ✅

  • Anil Choudhary
    August 24, 2025

    Separate proteins exclusively on the basis of molecular weight

  • Bhavana kankhedia
    August 24, 2025

    Separate proteins exclusively on the basis of molecular weight.

  • Niti Tanwar
    August 24, 2025

    Answer d is right

  • Alec
    August 24, 2025

    seperate proteins on the basis of their molecular weights.

  • Sonam Saini
    August 24, 2025

    Separation of proteins exclusively molecular weight

  • Anju
    August 24, 2025

    Ans:d
    SDS is a detergent and it degrades proteins

  • Anjali
    August 24, 2025

    Answer 4 is correct as sds provide uniform negative charge on protein, further they are separated on the basis on molecular weight

  • Shivani Panwar
    August 24, 2025

    Separate protein exclusively on the basis of molecular weight….

  • Devika
    August 24, 2025

    Separate proteins exclusively according to their molecular weight

  • Falguni Prakash
    August 24, 2025

    Sds helps in separation on the basis of mol wt

  • Kashish yadav
    August 25, 2025

    👍👍

  • Ayush Dubey
    August 25, 2025

    Separate proteins exclusively on the basis of molecular weight.

    • Patel Radha 3
      August 30, 2025

      Separate proteins exclusively on the basis of molecular weight.

  • sakshi khatri
    August 25, 2025

    done

  • Vijay kumar Meena
    August 25, 2025

    D ✔️

  • Pratibha Jain
    August 30, 2025

    Correct answer is option (d)
    Separate proteins exclusively on the basis of molecular weight.

  • Shriyanshi verma
    August 30, 2025

    Sds seprate protein based on their molecular weight

  • Sheikh Aleen
    August 30, 2025

    Separate proteins exclusively on the basis of molecular weight

  • Komal Sharma
    August 31, 2025

    Separate proteins exclusively on the basis of molecular weight

  • Vanshika Sharma
    August 31, 2025

    Seperate protiens exclusively on the basis of molecular weight

  • Shobha Kanwar
    August 31, 2025

    Separate proteins exclusively on the basis of molecular weight

  • Aartii sharma
    September 1, 2025

    Separate protein exclusively on the basis of their molecular weight

  • Anisha jakhar
    September 3, 2025

    Separate proteins exclusively on the basis of molecular weight.

  • Muskan Yadav
    September 3, 2025

    Separate proteins exclusively on the basis of molecular weight.

  • Somya Yadav
    September 5, 2025

    Separate protein on the basis of their mol. Wt.

  • Deeksha jharwal
    September 10, 2025

    Seprate protein on the basis of molecular weight

  • Meera Gurjar
    September 13, 2025

    D is right

  • Aakansha sharma Sharma
    October 1, 2025

    (d) Separate proteins exclusively on the basis of molecular weight

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