By adding SDS (sodium dodecyl sulfate) during the electrophoresis of proteins, it is possible to:
(a) Determine a protein’s isoelectric point.
(b) Determine an enzyme’s specific activity.
(c) Determine the amino acid composition of the protein.
(d) Separate proteins exclusively on the basis of molecular weight.
The correct answer is:
(d) Separate proteins exclusively on the basis of molecular weight.
Explanation:
Sodium dodecyl sulfate (SDS) is an anionic detergent used in SDS-PAGE (SDS-polyacrylamide gel electrophoresis). It denatures proteins by disrupting non-covalent bonds and imparts a uniform negative charge to all proteins. This ensures that proteins migrate through the gel based solely on their molecular weight, rather than their shape or charge.
- (a) Incorrect: Isoelectric focusing (IEF), not SDS-PAGE, is used to determine a protein’s isoelectric point (pI). SDS masks the protein’s charge, making pI determination impossible.
- (b) Incorrect: Enzyme activity is not assessed through SDS-PAGE, as SDS denatures proteins, making them inactive.
- (c) Incorrect: SDS-PAGE does not provide information about amino acid composition; techniques like mass spectrometry or amino acid analysis are used for that.
83 Comments
Pooja jat
February 27, 2025Done.,, easy one.
Lavanya Sharma
February 27, 2025✅👍🏻
Mahima Sharma
August 24, 2025D
Lavanya Sharma
February 27, 2025😄👍🏻
Prami Masih
February 27, 2025Good website
yogesh sharma
February 27, 2025Done sir
Lokesh kumawat
March 1, 2025Done
Abhilasha
March 1, 2025Easy done👍🏻
Nisha
March 1, 2025Well structured learning content
Suman bhakar
March 3, 2025👍👍
Priyanka choudhary
August 14, 2025Right answer is option d,sds separation by mw
VIKRAM GAHLOT
March 7, 2025👍
Arushi
April 1, 2025👍✔️
Anita choudhary
April 19, 2025☑️👍
Dharmpal Swami
August 13, 2025Sds seprate protein based on their molecular weight
Deepika sheoran
August 13, 2025Option d is correct answer
Because saparate protein migrate the gel based on their molecular weight.
Deepika sheoran
August 13, 2025Sds saprate protein migrate the gel based on their molecular weight.
Aman Choudhary
August 13, 2025SDS denature the protein and give uniform negative charge to all proteins by which identified on the basis of molecular weight not on charge and shape
Neelam Sharma
August 14, 2025Separate proteins exclusively on the basis of molecular weight.
Santosh Saini
August 14, 2025Seperation of protein on the basis of mole. Weight
Anurag Giri
August 14, 2025Sds provides uniform negative charge to all proteins. This ensures that proteins migrate through the gel based solely on their molecular weight, rather than their shape or charge
Anjani sharma
August 14, 2025Answer 4 is correct as sds provide uniform negative charge on protein, further they are separated on the basis on molecular weight
Karishma Don
August 14, 2025SDS denatures the protein and also give -ve charge so the protein migrates according to mol. weight only.
Khushi Agarwal
August 14, 2025Option d is correct answer
Sds seperates by mw
Khushi Singh
August 14, 2025Done
Soniya Shekhawat
August 14, 2025Easy sir😃
Urmi sisodiya
August 14, 2025Option d is correct bcoz SDS seperate protein based on their molecular weight
Khushi Mehra
August 14, 2025SDS produce uniform negative charge on protein and this protein get separated by the SDS PAGE technique
Avni
August 14, 2025Correct option is d
Separate proteins on the basis of molecular weight.
Nilofar Khan
August 14, 2025Correct answer is d
Roopal Sharma
August 14, 2025SDS separate protein on basis of molecular weight
Payal
August 14, 2025Option D separate protein on the basis of molecular weight.
Priya dhakad
August 14, 2025Option d is correct separation of protein on the basis of mol. Wt.
Deepika Sheoran
August 14, 2025SDS saprate protein on the basis of molecular weight.
Mansukh Kapoor
August 14, 2025The correct answer is option 4th
SDS helps into separation on the basis of molecular weight
Monika jangid
August 14, 2025Sds seprate protein on the basis on mw
Mohd juber Ali
August 14, 2025Option (4)
Sodium dodecyl sulphate use in SDS page it is protein denature detergent separate protein on the basis of mass
Dipti Sharma
August 14, 2025(d) Separate proteins exclusively on the basis of molecular weight
Surbhi Rajawat
August 14, 2025SDS treatment is basically given to impart negative charge on protein so that we can run it on PAGE and separate it on the basis of molecular weight
Sneha Kumawat
August 14, 2025Correct answer is for sds separate proteins by molecular weight
Shivani
August 14, 2025Option d is correct.
After adding sds it is possible to seprate protein exclusively on the basis of molecular weight
Priya khandal
August 14, 2025Option d is right molecular weight ke according
Ankita Pareek
August 15, 2025By sds page proteins separate on the basis of their molecular weight
Kajal
August 15, 2025Separate protein exclusively acc. To their molecular weight
Kavita choudhary
August 15, 2025Answer is d
shruti sharma
August 16, 2025option d
Aafreen
August 17, 2025Separate proteins exclusively on the basis of molecular weight
Neeraj Sharma
August 17, 2025SDS will provide negative charge on the proteins hence it will help in separation of proteins on the basis of molecular weight
Simran Saini
August 18, 2025Option d.
Separate proteins exclusively on the basis of molecular weight.
Seema
August 18, 2025D
Varsha Tatla
August 23, 2025D option will be correct
Shrddha Modi
August 23, 2025It’s just two determine sample on the basis of molecular weight OP. D
Bharti Yadav
August 24, 2025Separate proteins exclusively on the basis of molecular weight.
Priyanka Choudhary
August 24, 2025Answer d is correct answer,Separate proteins exclusively on the basis of molecular weight
MOHIT AKHAND
August 24, 2025Done sir ✅
Anil Choudhary
August 24, 2025Separate proteins exclusively on the basis of molecular weight
Bhavana kankhedia
August 24, 2025Separate proteins exclusively on the basis of molecular weight.
Niti Tanwar
August 24, 2025Answer d is right
Alec
August 24, 2025seperate proteins on the basis of their molecular weights.
Sonam Saini
August 24, 2025Separation of proteins exclusively molecular weight
Anju
August 24, 2025Ans:d
SDS is a detergent and it degrades proteins
Anjali
August 24, 2025Answer 4 is correct as sds provide uniform negative charge on protein, further they are separated on the basis on molecular weight
Shivani Panwar
August 24, 2025Separate protein exclusively on the basis of molecular weight….
Devika
August 24, 2025Separate proteins exclusively according to their molecular weight
Falguni Prakash
August 24, 2025Sds helps in separation on the basis of mol wt
Kashish yadav
August 25, 2025👍👍
Ayush Dubey
August 25, 2025Separate proteins exclusively on the basis of molecular weight.
Patel Radha 3
August 30, 2025Separate proteins exclusively on the basis of molecular weight.
sakshi khatri
August 25, 2025done
Vijay kumar Meena
August 25, 2025D ✔️
Pratibha Jain
August 30, 2025Correct answer is option (d)
Separate proteins exclusively on the basis of molecular weight.
Shriyanshi verma
August 30, 2025Sds seprate protein based on their molecular weight
Sheikh Aleen
August 30, 2025Separate proteins exclusively on the basis of molecular weight
Komal Sharma
August 31, 2025Separate proteins exclusively on the basis of molecular weight
Vanshika Sharma
August 31, 2025Seperate protiens exclusively on the basis of molecular weight
Shobha Kanwar
August 31, 2025Separate proteins exclusively on the basis of molecular weight
Aartii sharma
September 1, 2025Separate protein exclusively on the basis of their molecular weight
Anisha jakhar
September 3, 2025Separate proteins exclusively on the basis of molecular weight.
Muskan Yadav
September 3, 2025Separate proteins exclusively on the basis of molecular weight.
Somya Yadav
September 5, 2025Separate protein on the basis of their mol. Wt.
Deeksha jharwal
September 10, 2025Seprate protein on the basis of molecular weight
Meera Gurjar
September 13, 2025D is right
Aakansha sharma Sharma
October 1, 2025(d) Separate proteins exclusively on the basis of molecular weight