To determine the isoelectric point of a protein, first establish that a gel:

🙋‍♀️

👍👍

Ans b Exhibits a stable pH gradient when ampholytes become distributed in an electric field

Option B is correct
for determination of pi we need Ph gradient to stabilize the protein

ampholytes creates a stable ph gradient in electric field

Option b is correct answer
Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

The correct answer is option 2nd
Exhibits the stable pH gradient when ampholytes become disturbed in an electric field because at a point they stops migrating due to the isoelectric point

(b) Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Option (2) is right
Exhibit the stable pH gradient when ampholytes become disturbed
In low pH (+ve charge) protein move toward cathode electrod in pH strip (horizontal gel)
pH = PI (static) neutral

The correct answer is B, exhibits a stable pH gradient when ampholytes become distributed in an electric field

B is right

b is right

Ans- b
Proteins migrate within the gradient until they reach their isoelectric point (pI), where their net charge becomes zero and movement stops

(b) is correct

Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Done sir ✅

Answer b is correct answer,Exhibits a stable pH gradient when ampholytes become distributed in an electric field

Exhibits a stable pH gradient when ampholytes become distributed in an electric field. option b is correct.

exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Option B is right

Ans is b

Option b is correct
Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Exhibit a stable ph gradient when amhpholytes become distributed in an electric field

2

done

The correct answer is option (b)
Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Ans b Exhibits a stable pH gradient when ampholytes become distributed in an electric field

Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Done

To determine the isoelectric point (PI) of a protein isoelectric focusing (IEF) is used . IEF relies on a stable pH gradient created by ampholytes electric field .
Protein migrate with in the gradient untill they reach their isoelectric point ( PI) , where their net charge becomes zero and movement stops .

Option b is correct answer
Exhibits a stable pH gradient when ampholytes become distributed in an electric field

The correct answer is:

(b) Exhibits a stable pH gradient when ampholytes become distributed in an electric field.