In SDS PAGE of protein molecule separation, one SDS molecule will bind to
(a) Every amino acid
(b) Every two amino acids
(c) Every three amino acids
(d) Every four amino acids
(b) Every two amino acids ✅
Explanation:
In SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), SDS (Sodium Dodecyl Sulfate) binds to proteins in a ratio of approximately 1.4 g SDS per 1 g of protein, which translates to one SDS molecule binding to roughly every two amino acids.
Why is SDS Binding Important?
- Denaturation: SDS disrupts non-covalent interactions, unfolding proteins into linear polypeptides.
- Uniform negative charge: SDS imparts a consistent negative charge-to-mass ratio, ensuring that protein migration in the gel is based on size (molecular weight) rather than charge.
Why not the other options?
- (a) Every amino acid → Overestimates SDS binding; SDS binds at a lower frequency.
- (c) Every three amino acids → Underestimates the actual binding ratio.
- (d) Every four amino acids → Significantly underestimates the SDS binding rate.
Thus, the correct answer is (b) Every two amino acids. ✅
75 Comments
Suman bhakar
February 26, 2025Right
Mahima Sharma
August 24, 2025Every 2 molecule
Arushi
February 26, 2025☑️
Parul
February 27, 2025Easy. Done sir
Pooja jat
February 27, 2025Done .
Lavanya Sharma
February 27, 2025Got it sir!
Lavanya Sharma
February 27, 2025👍🏻🙏🏻😄Very nice explaining sir.
Prami Masih
February 27, 2025Well done 👍
Prami Masih
February 27, 2025Well done sir
Akshay mahawar
February 27, 2025Done👍
yogesh sharma
February 27, 2025Easily explained sir
Lokesh kumawat
March 1, 2025Done
Abhilasha
March 1, 2025Done sir
Nisha
March 1, 2025Understood
VIKRAM GAHLOT
March 7, 2025ok sir
Anita choudhary
April 19, 2025Done👍
Deepika sheoran
August 13, 2025Option b is correct answer
Because one sds molecule binding to roughly every two Amino acids.
Deepika sheoran
August 13, 2025One sds molecule binding to roughly every two Amino acids.
Santosh Saini
August 14, 2025Every 2 amino acid
Mitali
August 14, 2025(b) Every two amino acids ✅
Soniya Shekhawat
August 14, 2025Got it sir
Khushi Mehra
August 14, 2025Option b
Every 2 amino acid
Urmi sisodiya
August 14, 20252 amino acids
Roopal Sharma
August 14, 2025Every 2 amino acid
Deepika Sheoran
August 14, 2025Every two Amino acids.
Mohd juber Ali
August 14, 2025One SDS molecule bind to Every two a.a.
Dipti Sharma
August 14, 2025(b) Every two amino acids.
Priyanka choudhary
August 14, 2025Every 4 emino acid
Monika jangid
August 14, 2025Sds molecule will bind to 2 amino acids
Surbhi Rajawat
August 14, 2025I Did not know this, mentioning it right away in my notes.
Sneha Kumawat
August 14, 2025The correct answer is every two amino acid
Shivani
August 14, 2025One sds molecule will binds to every two amino acid
Priya khandal
August 14, 2025Every to Amino acid
Kajal
August 15, 2025Every two amino acid
Kavita choudhary
August 15, 2025Answer is b because sds bind every two amino acid
shruti sharma
August 16, 2025two amino acid
Kavita choudhary
August 17, 2025Every two amino acids
Aafreen
August 17, 2025Ans – Every two amino acids
Avni
August 17, 2025one SDS molecule bind to every two amino acids
Neeraj Sharma
August 17, 2025Option B as one SDS will provide negative charge to two amino acids
Simran Saini
August 18, 2025Every two amino acids.
Varsha Tatla
August 23, 20251.4 g SDS /1g protein
Or 2 amino acid
Bharti Yadav
August 24, 2025One SDS molecule bind to every two amino acids
karishma don
August 24, 2025one sds molecule binds with every 2 amino acid
Priyanka Choudhary
August 24, 2025Sds molecul will bind to every two amino acid
MOHIT AKHAND
August 24, 2025Done sir ✅
Priya dhakad
August 24, 2025In SDS PAGE of protein molecule separation, one SDS molecule will bind to every 2 amino acids .
Anil Choudhary
August 24, 2025Every two amino acids
Bhavana kankhedia
August 24, 2025Every two amino acids.
Niti Tanwar
August 24, 2025Every 2 amino acid
Alec
August 24, 2025one SDS molecule will bind to every two amino acids.
Sonam Saini
August 24, 2025Every 2 amino acid
Anju
August 24, 2025Ans:b
1SDS binds every 2 AA
Anjali
August 24, 2025Every 2 amino acid
Seema
August 24, 2025B)one sds molecule will bind with 2 amino acid .
Shivani Panwar
August 24, 2025Every two amino acid
Devika
August 24, 2025One sodiumdodecyl sulphate molecule binds to every two amino acids
Falguni Prakash
August 24, 2025Every 2 Aa
Ayush Dubey
August 25, 2025Every two amino acids
sakshi khatri
August 25, 2025done
Pratibha Jain
August 30, 2025Correct answer is option (b)
Every two amino acids
Shriyanshi verma
August 30, 2025One sds molecule binding to roughly every two Amino acids.
Sheikh Aleen
August 30, 2025Every two amino acids
Patel Radha
August 30, 2025Every 2 amino acid
anjani sharma
August 30, 2025(b) Every two amino acids.
Komal Sharma
August 31, 2025In SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), SDS (Sodium Dodecyl Sulfate) binds to proteins in a ratio of approximately 1.4 g SDS per 1 g of protein, which translates to one SDS molecule binding to roughly every two amino acids.
Vanshika Sharma
August 31, 2025Binds with every tow amino acid
Shobha Kanwar
August 31, 2025The correct answer is every two amino acid
Aartii sharma
September 1, 2025Every two amino acid
Anisha jakhar
September 3, 2025One SDS binds to every two amino acids
Muskan Yadav
September 3, 2025SDS molecule binding to roughly every two amino acids .
Somya Yadav
September 5, 2025Every two amino acid
Deeksha jharwal
September 10, 2025One SDS binds binds to every two amino acids
Meera Gurjar
September 13, 2025Every two amino acids
Aakansha sharma Sharma
October 1, 2025Option b is correct answer
Because one sds molecule binding to roughly every two Amino acids.