8. Among the following enzyme whirls is not involved in DNA replication process-
(1) Primase
(2) RNA polymerase
(3) DNA polymerase
(4) Helicase
Introduction
DNA replication is a complex and highly regulated process that involves a suite of specialized enzymes working in concert to accurately duplicate the genome. Key enzymes such as primase, DNA polymerase, and helicase are essential for unwinding the DNA, synthesizing primers, and elongating new DNA strands. However, not all enzymes that interact with nucleic acids participate in DNA replication. This article explains why RNA polymerase is not involved in DNA replication, distinguishing its role from those of replication-specific enzymes.
Enzymes Involved in DNA Replication
1. Primase
-
Synthesizes short RNA primers complementary to the DNA template.
-
Provides a free 3′-OH group necessary for DNA polymerase to begin DNA synthesis.
-
Essential for initiating synthesis on both leading and lagging strands.
2. DNA Polymerase
-
Catalyzes the addition of deoxynucleotides to the growing DNA strand.
-
Possesses proofreading activity to ensure replication fidelity.
-
DNA polymerase III is the main enzyme responsible for DNA synthesis in prokaryotes.
3. Helicase
-
Unwinds the double-stranded DNA helix ahead of the replication fork.
-
Breaks hydrogen bonds between complementary bases to expose single-stranded templates.
-
Enables replication machinery to access DNA strands for synthesis.
Why RNA Polymerase Is Not Involved in DNA Replication
-
RNA polymerase is the enzyme responsible for transcription, the synthesis of RNA from a DNA template.
-
It synthesizes RNA molecules such as mRNA, tRNA, and rRNA.
-
RNA polymerase does not synthesize DNA or participate in the replication fork machinery.
-
Although primase synthesizes RNA primers, it is a specialized RNA polymerase dedicated to replication, distinct from the RNA polymerase involved in transcription.
Summary Table
| Enzyme | Role in DNA Replication Process | Involved in DNA Replication? |
|---|---|---|
| Primase | Synthesizes RNA primers for DNA polymerase | Yes |
| DNA Polymerase | Synthesizes new DNA strands | Yes |
| Helicase | Unwinds DNA double helix | Yes |
| RNA Polymerase | Synthesizes RNA during transcription | No |
Conclusion
Among the listed enzymes, RNA polymerase is not involved in the DNA replication process. Instead, DNA replication relies on primase, DNA polymerase, and helicase to unwind DNA, synthesize primers, and elongate new DNA strands. RNA polymerase’s role is confined to transcription, making it distinct from the replication machinery.
Answer:
The enzyme not involved in DNA replication is (2) RNA polymerase.
43 Comments
Manisha choudhary
July 25, 2025😄😃😀
Vijay Marmat
August 2, 2025Rna polymerase enzyme not involve dna replication
Diksha chhipa
July 29, 2025DNA pol 3 synthesis of new dna strand have proof reading activity
Helicase unwind the both strand of dna
Primerase make rna primer for dna polymerase .
RNA pol is not required for dna replication process .it is used in rna synthesis
Mansukh Kapoor
July 29, 2025Correct answer is RNA polymerase because it is not used in DNA replication rather than this enzymes which are given are used in DNA REPLICATION
Pratibha Sethiya
July 29, 2025Correct option is option (2) RNA polymerase
Bcz this enzyme is responsible for transcription, synthesize RNA from DNA template it does not synthesize DNA or participate in Replication process, although
Primase is specialised RNA polymerase dedicated to Replication it synthesize primers ,
Khushi Agarwal
July 29, 2025Rna pol is not used in dna replication it is used in rna synthesis
Payal Gaur
July 29, 2025RNA polymerase not required for DNA replication.
Primase = rna primer add (initiation of dna replication)
Helicase = unwind double strand DNA.
DNA polymerase = synthesis of DNA .
anurag giri
July 29, 2025Ans 2 RNA polymerase is the enzyme responsible for transcription
Vanshika Sharma
July 29, 2025Correct answer is Rna polymerase bcz it’ is not involved in dna replication rather than it used to synthesize rna during transcription
Surbhi Rajawat
July 29, 2025RNA polymerase is used in the process of transcription and not replication. Hence 2nd is the answer
HIMANI FAUJDAR
July 29, 2025Correct answer is RNA polymerase because it is used in transcription for synthesis of RNA from DNA
Heena
July 29, 2025Rna polymerase isn’t involved in dna replication process
Priti Khandal
July 29, 2025Option b is right bcz rna polymer ase dna replication me help nahi karta heli case dna polymerase and promise dna replication me help karta hai
Priya Khandal
July 29, 2025Option B is right just because DNA polymerase and and helicate and primers are must required d doesn’t required RNA polymerase
shruti sharma
July 29, 2025RNA pol is not involved in dna replication process
Khushi Mehra
July 29, 2025RNA polymerase is used for RNA synthesis….primase work as a RNA polymerase
Therefore correct ans is 2
Priyanka choudhary
July 29, 2025The right answer is RNA polymerase, because
Transcription m helpfull hota h replication m nhi
Neelam Sharma
July 29, 2025Clear cut sir 😁
Neelam Sharma
July 29, 2025RNA polymerase
Monika jangid
July 29, 2025Rna polymerase is not involved in dna replication
Priya Dhakad
July 30, 2025The answer is RNA polymerase is not involved in DNA replication, it is used in RNA synthesis
Sneha Kumawat
July 30, 2025RNA polymerase because involved in transcription
Payal Gaur
July 30, 2025RNA polymerase not involved in DNA replication
Aafreen
July 30, 2025Correct answer is 2 :- RNA polymerase is not involved in DNA replication
Aafreen
July 30, 2025Correct answer is 2 :- RNA polymerase is not involved in DNA Replication . It involves in Transcription
Aafreen Khan
July 30, 2025Correct answer is 2 :- RNA polymerase is not involved in DNA Replication . It involves in Transcription
Aafreen
July 30, 2025Correct answer is 2
Aafreen
July 30, 2025Correct answer is 2 Correct answer is 2 :- RNA polymerase is not involved in DNA Replication
Niti tanwar
July 30, 2025Rna pol. is not involved dna replication
Deepika Sheoran
July 30, 2025Rna polymerase is not involved in Dna replication.
Aman Choudhary
July 30, 2025Rna polymerase bcz it involved in transcription
Khushi Vaishnav
July 31, 2025RNA polymerase is not involved in the DNA replication process.
Anisha jakhar
July 31, 2025RNA polymerase is not involved in DNA replication.
Shivani
July 31, 2025RNA polymerase is used for RNA synthesis…
The correct answer is 2 👍
Avni
July 31, 2025the enzyme not involved in DNA replication is RNA polymerase as it is involved in transcription
Dipti Sharma
July 31, 2025RNA polymerase is not involved in dna replication , its role is confined to transcription.
However, Primase involves in primer formation during replication.
Dharmpal Swami
August 1, 2025Rna polymerase absent in replication
Mahima Sharma
August 2, 2025Rna polymerase required for rna synthesis.
Soniya Shekhawat
August 3, 2025RNA polymerase is not involved in replication, involved in transcription.
Swati Choudhary
August 4, 2025RNA. Pol is not involved in rep.
Anita Choudhary
August 28, 2025RNA Polymerase is not involved in replication it involve in transcription.
Alec
August 30, 2025Rna polymerase is not involved in Dna replication.
Deepika Sheoran
November 6, 2025RNA polymerase is not involved in DNA replication.