To determine the isoelectric point of a protein, first establish that a gel:

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Done ✅ sir

Answer b is correct as in isoelectric focusing point the proteins migrate with ph gradient until net charge is zero

It is achieved using ampholytes, which establish a stable pH gradient when an electric field is applied.

(b) Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Ist occuring IEF in which protein are separate on the basis of PH with the net charge of protein is be have zero so B is correct option .

IEF in which protein are separate on the based of PH (ampholytes )with the net charge of protein is be have zero so B is correct option .

Iso electric point refers to the migration of protein within the gradient until the net charge on protein becomes zero . This can be done by adding ampholytes which can act as an acid as well as base.
Correct option is b

Option b is correct bcoz net charge of proteins have to be zero

Option b is correct
Because isoelectric focusing proteins move through a ph gradient until they reach a point where their net charge is zero.

B is correct as protein is separated on the basis of oh gradient

To determine isoelectric point we must have a Ph gradient which will help in neutralizing the protein therefore B is correct option

Option b is correct 👍

b is correct 👍

Option b is correct .

Exhibits a stable pH gradient when ampnolytesbecome distrbuted in a electric field

To determine isoelectric point isoelectric focusing is used where proteins separate on the basis of their.. charge on proteins depends on ph ief is relies on stable ph gradient so option b is correct

Exhibit a stable ph gradient when ampholyte gets distributed acc. To the PH gradient

Answer is b

B is right answer because IEF is on stable pH gradient and in electric field so charge is important

Option b as we need a stable ph gradient to find the isoelectric point

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Amphobialite is a word that can donate and accept iones pi is the ph at which molecule has no net electric charge

Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Done sir ✅

B is correct answer,Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Option b is correct because net charge of proteins have to be 0

Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

ans is b – establish a stable ph gradient when ampholytes are distributed in the electric field.

Ans:B
In Isoelectric focusing protein moves with ph gradient till the net charge is zero

Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Answer B…👍

B is correct

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Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

done

Option b is correct bcoz net charge of proteins have to be zero

(b) Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Exhibits a stable pH gradient when ampholytes become distributed in an electric field.

Option b is correct
Because isoelectric focusing proteins move through a ph gradient until they reach a point where their net charge is zero.

Option b is correct
Because isoelectric focusing proteins move through a ph gradient until they reach a point where their net charge is zero.

B Option is correct