The main advantage of discontinuous buffer system in SDS and native PAGE is
(a) Conformation of protein is conserved
(b) Constantly maintain the charge of proteins
(c) Assist in migration of proteins
(d) Enhance resolution of separation
The correct answer is:
(d) Enhance resolution of separation
Explanation:
A discontinuous buffer system is commonly used in SDS-PAGE and native PAGE to improve the resolution of protein separation. This system consists of:
- Stacking gel (low acrylamide concentration, pH ~6.8)
- Resolving gel (higher acrylamide concentration, pH ~8.8)
- Electrode buffer (higher ionic strength, pH ~8.3)
Why does it enhance resolution?
- In the stacking gel, proteins are concentrated into thin bands due to differences in ion mobility, creating a sharp starting zone.
- In the resolving gel, proteins separate based on size (SDS-PAGE) or charge (native PAGE), leading to better resolution and clearer band separation.
Why not the other options?
- (a) Conformation of protein is conserved → This applies to native PAGE, but a discontinuous buffer system is primarily used to enhance resolution.
- (b) Constantly maintain the charge of proteins → While buffer systems influence protein charge, the main purpose of discontinuous buffers is improving separation, not just maintaining charge.
- (c) Assist in migration of proteins → Buffers do help migration, but the primary benefit is sharp band formation and high resolution.
Thus, the correct answer is (d) Enhance resolution of separation ✅
59 Comments
Priti khandal
February 26, 2025Ok sir
Prami Masih
February 27, 2025Got it sir
Mahima Sharma
August 24, 2025Done
Arushi
April 1, 2025👍✔️
Santosh Saini
August 14, 2025Option 4th is correct bcz discontinuous buffer system (2 different concentrations, one is stacking low con. 1/3and 2nd is resolving high con. 2/3) is used in SDS and native page
Soniya Shekhawat
August 14, 2025Discontinuous buffer system is enhance the resolution of separation because in which two different concentration of gel is present one is resolving gel which is the 2/3 and another is a streaking gel 1/3.
Khushi Agarwal
August 14, 2025Enhance resolution of separation
Dharmpal Swami
August 14, 2025Enhance resolution of separation
Neelam Sharma
August 14, 2025Enhance resolution of separation because discountinous slab separation. It is best separation tech. for cleared band separation
Anjani sharma
August 14, 2025Enhanced the resolution of separation, answer 4 is correct
Surbhi Rajawat
August 14, 2025In discontinuous gel due to difference in pore size we will be able to resolve proteins of different size in a better and more efficient way
Karishma Don
August 14, 2025Advantage- helps in migration and also enhance the resolution of separation
Mitali
August 14, 2025, the correct answer is (d) Enhance resolution of separation ✅
Nilofar Khan
August 14, 2025Correct answer is d 💯
Roopal Sharma
August 14, 2025Enhancedthe resolution of separation
Priya dhakad
August 14, 2025Enhance the resolution of separation becoz 2 different concentration of gel is present. First is stacking gel and 2 nd is resolving gel.
Mansukh Kapoor
August 14, 2025The correct answer is option 4th
The advantage of the discontinuous buffer is to enhance the resolution of the protein
Deepika Sheoran
August 14, 2025Enhanced the resolution of saparation.
Mohd juber Ali
August 14, 2025Option (4) is right answer
Enhance the resolution of protein
Dipti Sharma
August 14, 2025correct answer is d
enhance resolution of separation .
Monika jangid
August 14, 2025Enhance resolution separation
Priyanka choudhary
August 14, 2025Correct answer is enhance resolution of separation
Sneha Kumawat
August 14, 2025Enhance resolution of separation
Surbhi Rajawat
August 14, 2025Discontinuous gel helps in better resolution of various sizes of protein due to difference in the pore size of stacking and resolving gel
Parul Yadav
August 14, 2025Discontinuous buffer system in SDS-PAGE and native PAGE improves the resolution of protein separation.
Shivani
August 14, 2025Option d is correct. ( Enhance resolution of sepration )
Priya khandal
August 14, 2025Enhance the regulation of separation
Kajal
August 15, 2025Enhance resolution of separation
shruti sharma
August 16, 2025enhance resolution of separation
Aafreen
August 17, 2025Enhance resolution of separation
Avni
August 17, 2025the correct answer is (d) Enhance resolution of separation
Neeraj Sharma
August 17, 2025Option D is correct it enhances the resolution of separation
Varsha Tatla
August 23, 2025Advantage of Discouninuous buffer in SDS n page- resolution inhance
Bharti Yadav
August 24, 2025Enhance resolution of separation
Priyanka Choudhary
August 24, 2025The right answer is option d,
Enhance resolution of separation
MOHIT AKHAND
August 24, 2025Done sir ✅
Niti Tanwar
August 24, 2025Enhance resolution of separation
Alec
August 24, 2025d – enhances resolution of seperation
Sonam Saini
August 24, 2025Enhanced resolution of separation
Anjali
August 24, 2025Ans is 4.The advantage of the discontinuous buffer is to enhance the resolution of the protein
Anju
August 24, 2025Ans:d
SDS -PAGE and native PAGE improve the resolution of protein separation
Seema
August 24, 2025Enhance resolution of saperation becouse of discontinus buffer .
Shivani Panwar
August 24, 2025Enhance resolution of separation
Devika
August 24, 2025Enhance resolution of separation
Falguni Prakash
August 24, 2025D
Kashish yadav
August 25, 2025Option d
Ayush Dubey
August 25, 2025Enhance resolution of separation
sakshi khatri
August 25, 2025done
Pratibha Jain
August 30, 2025Correct answer is option (d)
Enhance resolution of separation
Shriyanshi verma
August 30, 2025Enhanced the resolution of separation, answer 4 is correct
Sheikh Aleen
August 30, 2025Enhance resolution of separation
Patel Radha
August 30, 2025Correct answer is enhance resolution of separation
Shobha Kanwar
August 31, 2025Enhance resolution of separation because discountinous slab separation. It is best separation technique for cleared band separation
Aartii sharma
September 1, 2025Enhanced resolution of separation
Anisha jakhar
September 3, 2025Enhance resolution of separation
Muskan Yadav
September 3, 2025Enhance resolution of separation
Somya Yadav
September 5, 2025(d) Enhance resolution of separation ✅
Meera Gurjar
September 13, 2025Resolution of seperation
Aakansha sharma Sharma
October 1, 2025Enhance resolution of separation