Heating of some nucleic acids shows an increase in the absorbance at 260 nm (A260) typified by the plot shown above. The sharp transition midpoint is defined as melting temperature […]
Tag: Stability of Proteins PYQ
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CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
How Double-Stranded DNA Is Denatured: The Role of Formamide and Other Agents
Double stranded DNA can be destabilized and denatured by (1) Formamide (2) Polyglycine (3) Sucrose (4) PEG Introduction DNA denaturation involves the separation of the two complementary strands of the […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Understanding Differences in DNA Denaturation Profiles: Role of GC Content and Genome Complexity
Denaturation profiles of DNA are shown below The differences in the profiles arise because (1) the DNA is single stranded but of different sizes (2) A + T content of […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Which Graph Represents Single-Stranded DNA Concentration vs Decreasing Temperature During Renaturation?
The graph between the concentration of Single stranded DNA (Y-axis) and decrease in temperature (X- axis during renaturation of DNA can be represented as Analysis During DNA renaturation (as […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Understanding DNA Melting Temperature (Tm): Which Factor Does Not Influence It?
The Temperature of Melting (Tm) is defined as the temperature at which 50% of double stranded DNA is changed to single-standard DNA. Tm of DNA does not depends on (1) […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
How to Calculate DNA Denaturation Enthalpy (ΔH) at 37°C from Values at Higher Temperatures
It has been observed that for the DNA double helix melting, the value of ΔH (enthalpy change of denaturation) are 80 and 90 kcal/mole at 700 and 800, respectively. Assuming […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
How to Estimate Protein Stability Thermodynamics Using Equilibrium Constant and Temperature
Protein stability is represented as Prior to development of sensitive calorimeters, thermodynamic parameters of processes were determined by following equation ΔHO and ΔSO are standard changes m enthalpy and entropy, […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
How to Calculate Gibbs Free Energy Change (ΔG) of DNA Denaturation at 37°C from DSC Data
Differential scanning calorimetric study of calf thymus DNA was carried out to measure midpoint of thermal denaturation (Tm). ΔHm (enthalpy change at Tm) and ΔCP (constant-pressure heat capacity change). It […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Understanding SDS-Induced Protein Denaturation: Effects on Secondary and Tertiary Structures
Sodium dodecyl sulphate (SDS) denatures protein by altering their (1) Primary structure only (2) Primary and secondary structure (3) Secondary and tertiary structure (4) Primary, secondary and tertiary structure […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Which Guanidinium Salt Is the Most Effective Protein Denaturant? Understanding the Role of Counterions
Most effective protein denaturant form of guanidinium when used in equimolar concentration is (1) Iodide (2) Chloride (3) Bromide (4) Sulphate Introduction Guanidinium salts are widely used chemical denaturants […]
