Best Technique to Locate Developmental RNAs in Drosophila Embryo

1. Which of the following technique is most suitable to demonstrate that particular RNAs that are important for development are located in distinct regions off the Drosophila embryo ?

 

A. Western blotting
B. Northern blotting
C. In situ hybridization
D. In vitro translation

Best Technique to Locate Developmental RNAs in Drosophila Embryo

Technique to Locate Developmental RNAs is In Situ Hybridization: The Most Suitable Technique for RNA Localization

In developmental biology, understanding the spatial distribution of specific RNAs is crucial to deciphering gene expression patterns. Among the techniques available—Western blotting, Northern blotting, In situ hybridization, and In vitro translation—the most suitable method for demonstrating the localization of RNAs in distinct regions of the Drosophila embryo is In situ hybridization (ISH).

Why In Situ Hybridization?

In situ hybridization is a molecular technique used to detect specific RNA or DNA sequences within tissue sections or whole embryos. This method involves hybridizing a labeled complementary probe (DNA or RNA) to the target RNA within cells, thereby allowing visualization of spatial expression patterns.

  1. Spatial Resolution:

    • Unlike Northern blotting, which provides only quantitative data without spatial context, ISH allows visualization of where the RNA is localized within the embryo.
  2. High Specificity:

    • The technique employs complementary probes that specifically bind to the RNA of interest, ensuring accuracy in detection.
  3. Single-Cell Resolution:

    • ISH can pinpoint RNA localization at the single-cell level, making it an ideal choice for studying early development in Drosophila.
  4. Application in Developmental Biology:

    • Many Drosophila genes, like bicoid (bcd) and nanos (nos), exhibit region-specific expression crucial for axis formation. ISH helps map these RNAs in the embryo, providing critical insights into their function.

Why Other Techniques Are Less Suitable?

  • Western Blotting: Detects proteins, not RNA, making it irrelevant in this context.
  • Northern Blotting: Detects RNA but lacks spatial resolution.
  • In Vitro Translation: Analyzes protein synthesis but does not provide RNA localization data.

Conclusion

For detecting and visualizing RNAs crucial for Drosophila embryonic development, In situ hybridization (ISH) is the most appropriate technique due to its spatial specificity, high resolution, and ability to provide direct localization insights.

104 Comments
  • Shreeji Charan
    March 1, 2025

    In situ hybridization

  • Suman bhakar
    March 3, 2025

    Ok

  • Arushi
    March 3, 2025

    👍👍

    • Kavita choudhary
      August 20, 2025

      In situ hybridization

  • Prami Masih
    March 4, 2025

    Done sir ✅

  • Aafreen
    August 20, 2025

    Ans-3 In-Situ Hybridization
    In-Situ Hybridization detect specific DNA or RNA sequence and labelled complementary probe to the RNA with in the cells
    Northern blotting also detected RNA but lack of localisation

  • Sakshi yadav
    August 20, 2025

    In situ hybridization

  • Priya choudhary
    August 20, 2025

    Correct answer is in situ hybridization

  • Neelam Sharma
    August 20, 2025

    In situ hybridization to detect RNA location in embryo

  • Neelam Sharma
    August 20, 2025

    In situ hybridization

  • shruti sharma
    August 20, 2025

    in situ hybridization

  • Mitali Saini
    August 20, 2025

    For detecting and visualizing RNAs crucial for Drosophila embryonic development, In situ hybridization (ISH) is the most appropriate technique due to its spatial specificity, high resolution, and ability to provide direct localization insights.

  • Dipti Sharma
    August 20, 2025

    In situ hybridization for detecting particular rna .

  • Manisha gujar
    August 20, 2025

    In situ hybridisation

  • Manisha gujar
    August 20, 2025

    Insitu hybridisation

  • Manisha gujar
    August 20, 2025

    In situ hybridisation

  • Komal Soni
    August 20, 2025

    In situ hybridization to detection perticular RNA in distinct region

  • Shivani
    August 20, 2025

    In situ hybridization to detect perticular rna.
    Option C is correct .

  • Mansukh Kapoor
    August 20, 2025

    The correct answer is option 3rd
    In situ hybridization tech.is the best for the identification of a particular RNA in drosophila

  • Khushi Mehra
    August 20, 2025

    Answer is 3rd In situ hybridization
    It is a technique for detection of specific RNA inside the cell or embryo by adding labelled DNA probe of a specific sequence.

  • Ekta yadav
    August 20, 2025

    Insitu hybridisation

  • Varsha Tatla
    August 20, 2025

    In situ hybridization because it is a most appropriate tech for detection of rna in developing drosophila embryo.it is a crucial tech due to the ability to provide specific locatization site, high resolution, special specity so option in situ hybridization is a best answer

  • Diksha Chhipa
    August 20, 2025

    For rna in situ hybridisation

  • Aakansha Sharma
    August 20, 2025

    In situ hybridization

  • Mohd juber Ali
    August 20, 2025

    Option (3) right answer
    In situ hybridization is appropriate teq. For detecting and visualisation Rna for drosophila embryo

  • Dharmpal Swami
    August 20, 2025

    Ish technique use in drosophila for detecting perticular rna

  • Anurag Giri
    August 20, 2025

    Ans 3 in situ hybridisation
    Bcoz situ hybridization is the most appropriate technique specificity,and to provide direct localization insights.

  • Simran saini
    August 20, 2025

    In situ hybridization.

  • HIMANI FAUJDAR
    August 20, 2025

    Ans. In situ hybridization technique is suitable for detecting for drosophila embryonic development

  • Roopal Sharma
    August 20, 2025

    Insitu Hybridization technique is used for detecting spatial rna localisation

  • Bharti Yadav
    August 20, 2025

    In situ hybridization for detection of specific Rna sequence

  • Deepika Sheoran
    August 20, 2025

    Option 3 is correct
    Insitu hybridization to detect particular RNA.

  • Surbhi Rajawat
    August 20, 2025

    Among options available both insitu hybridization and northern blotting can be the answer but the former is preferred because it is highly sensitive and requires less effort

  • Aman Choudhary
    August 20, 2025

    Option 3 is correct because in-situ hybridization is used to detect the special Rna sequences

  • Surendra Dudi
    August 20, 2025

    In situ hybridization

  • Mahima Sharma
    August 20, 2025

    In situ hybridization tech . Best for detection of perticular rna

    • Nilofar Khan
      August 20, 2025

      In situ hybridization

  • Avni
    August 20, 2025

    In Situ Hybridization

  • Sneha .......
    August 20, 2025

    In situ hybridization

  • Priya dhakad
    August 20, 2025

    In situ hybridization is used for specific RNA.

  • anjani sharma
    August 20, 2025

    In situ hybridization

  • Manisha
    August 21, 2025

    In situ hybridization techniques used for detection RNAs crucial for drosophila development

  • Priyanka
    August 21, 2025

    Done option 3

  • Neha
    August 21, 2025

    Insitu hybridization as in this technique probe is directly given to the cell

  • Sakshi kanwar
    August 21, 2025

    In situ hybridization

  • Arushi
    August 21, 2025

    In situ hybridization

  • Sonam Saini
    August 21, 2025

    Done

  • Santosh Saini
    August 21, 2025

    In situ hybridization technique used for detection RNAs crucial for drosophila

    • Bhawna Choudhary
      August 21, 2025

      In situ hybridization

  • Khushi Pareek
    August 21, 2025

    In situ hybridization

  • Khushi Vaishnav
    August 21, 2025

    In situ hybridization is the most appropriate technique

  • Shivani
    August 21, 2025

    In situ hybridization 👍

  • Sakshi yadav
    August 21, 2025

    Insitu hybridization

  • Minal Sethi
    August 21, 2025

    In-Situ Hybridization

  • Divya rani
    August 21, 2025

    In situ hybridization is the detection technique of specific rna inside the cell or drosophila embryo cell.

  • Sonam Saini
    August 21, 2025

    Right is in situ hybridization because cell ke under embryo ke liye rna detect Krna h

  • Soniya Shekhawat
    August 22, 2025

    In situ hybridization is a technique to use to detect the specific RNA or dna within tissues and embryo in drosphila development.

  • Sheikh Aleen
    August 22, 2025

    In situ hybridization

  • Bhavana kankhedia
    August 22, 2025

    In situ hybridization

  • Khushi Singh
    August 22, 2025

    Ish is technique in which detection of particular rna inside cell which is suitable for embryonic devey

  • Kirti agarwal
    August 23, 2025

    In situ hybridization

  • Khushi Agarwal
    August 23, 2025

    Option 3 is correct answer
    Insitu hybridization is detected location of embro

  • Priya khandal
    August 23, 2025

    In situ hybridization

  • Niti Tanwar
    August 23, 2025

    In situ hybridization

  • Jyoti
    August 23, 2025

    In situ hybridization

  • karishma don
    August 23, 2025

    ISH due to its high resolution and detecting exact location

  • Kanishka Parnami
    August 23, 2025

    In situ hybridization allows us to locate RNA, gene location, duplication, deletion, translocation, inversion and many more.

  • Akshita Jangid
    August 23, 2025

    In situ hybridization is a technique that is used to detect specific RNA sequences within the tissues

  • Samiksha bajiya
    August 24, 2025

    In situ hybridization is a molecular technique used to detect specific RNA or DNA sequence within tissue section

  • Divya
    August 24, 2025

    In situ hybridization is right amswer

  • Karishma
    August 24, 2025

    In situ hybridization

  • Sonam Saini
    August 24, 2025

    In situ hybridization

  • Kajal
    August 24, 2025

    Correct answer is insitu Hybridization.

  • Yogita
    August 24, 2025

    In Situ hybridization

  • Aakanksha Sharma
    August 24, 2025

    In situ hybridization

  • Gunjan Singh
    August 24, 2025

    in situ hybridization

  • Himanshi
    August 24, 2025

    In situ hybridization

  • Anisha Beniwal
    August 24, 2025

    In situ hybridization as we want to know about the region in the embryo

  • Meenakshi Choudhary
    August 24, 2025

    In situ hybridization

  • Ayush
    August 24, 2025

    Option c
    In situ hybridization
    B/s it Uses labeled RNA/DNA probes to visualize specific RNA molecules directly in tissue or embryos, showing where in the Drosophila embryo the RNA is localized

  • AKANKSHA RAJPUT
    August 24, 2025

    In situ hybridization

  • Tanvi Panwar
    August 24, 2025

    Insitu hybridization technique is used to locate or detect DNA in particular chromosomes.

    • Tanvi Panwar
      August 24, 2025

      Insitu hybridization technique is used to locate or detect RNA in particular chromosomes.

      • Manisha choudhary
        August 26, 2025

        Western blotting s protein kaa detection hota h
        Northern blotting s rna kaa detection hota h y quantitative analysis k liye achha h pr Spatial resolution nhi deta h
        In situ hybridization s single cell m bhi rna ki location pta kr kte h jo drosophila development k liye important hote h axis formation k liye
        In situ hybridization Spatial resolution deta h
        In vitro translation s protein formation pta chlta h

  • Neeraj Sharma
    August 24, 2025

    Option C is right as in, in situ hybridization we can detect within the cell and northern blotting the detection is out of the cell so we cannot distinct the places

  • MOHIT AKHAND
    August 24, 2025

    Done sir ✅

  • Pooja Sharma
    August 24, 2025

    OPTION C IN SITU HYBRIDIZATION

  • Surendra Doodi
    August 24, 2025

    In situ hybridization

  • Falguni Prakash
    August 24, 2025

    Ish

  • Priyanka Meena
    August 26, 2025

    In situ hybridization

  • Monika jangid
    August 26, 2025

    In situ hybridization

  • Muskan Yadav
    August 26, 2025

    Answer is 3rd – In situ hybridization
    It is a technique for detection of specific RNA inside the cell or embryo by adding labelled DNA probe of a specific sequence.

  • Pratibha Jain
    August 30, 2025

    Correct answer is option (c)
    ISH is a technique to use to detect the specific RNA or dna within tissues and embryo in drosophila development.

  • Shriyanshi verma
    August 30, 2025

    In situ hybridization

  • Alec
    August 30, 2025

    In situ hybridization technique ismost suitable due it’s spatial stability, high resolution and ability to provide direct localization insights.

  • Asha Gurzzar
    August 30, 2025

    In situ hybridization

  • Manisha choudhary
    August 31, 2025

    Inse to hybridization

  • Manisha choudhary
    August 31, 2025

    In situ hybridization

  • Anisha jakhar
    August 31, 2025

    In situ hybridization

  • Kajal
    August 31, 2025

    In situ hybridization

  • Shobha Kanwar
    August 31, 2025

    In situ hybridization to detect RNA location in embryo

  • Kanica Sunwalka
    August 31, 2025

    in situ hybridization – detect specific rna /dna seq and hybridize labelled complementary probe to target rna within cell

  • Anjali
    September 1, 2025

    Insitu Hybridization technique is used for detecting spatial rna localisation

  • Deepika sheoran
    November 15, 2025

    Insitu hybridization

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