1. Which of the following technique is most suitable to demonstrate that particular RNAs that are important for development are located in distinct regions off the Drosophila embryo ?
A. Western blotting
B. Northern blotting
C. In situ hybridization
D. In vitro translation
Best Technique to Locate Developmental RNAs in Drosophila Embryo
Technique to Locate Developmental RNAs is In Situ Hybridization: The Most Suitable Technique for RNA Localization
In developmental biology, understanding the spatial distribution of specific RNAs is crucial to deciphering gene expression patterns. Among the techniques available—Western blotting, Northern blotting, In situ hybridization, and In vitro translation—the most suitable method for demonstrating the localization of RNAs in distinct regions of the Drosophila embryo is In situ hybridization (ISH).
Why In Situ Hybridization?
In situ hybridization is a molecular technique used to detect specific RNA or DNA sequences within tissue sections or whole embryos. This method involves hybridizing a labeled complementary probe (DNA or RNA) to the target RNA within cells, thereby allowing visualization of spatial expression patterns.
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Spatial Resolution:
- Unlike Northern blotting, which provides only quantitative data without spatial context, ISH allows visualization of where the RNA is localized within the embryo.
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High Specificity:
- The technique employs complementary probes that specifically bind to the RNA of interest, ensuring accuracy in detection.
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Single-Cell Resolution:
- ISH can pinpoint RNA localization at the single-cell level, making it an ideal choice for studying early development in Drosophila.
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Application in Developmental Biology:
- Many Drosophila genes, like bicoid (bcd) and nanos (nos), exhibit region-specific expression crucial for axis formation. ISH helps map these RNAs in the embryo, providing critical insights into their function.
Why Other Techniques Are Less Suitable?
- Western Blotting: Detects proteins, not RNA, making it irrelevant in this context.
- Northern Blotting: Detects RNA but lacks spatial resolution.
- In Vitro Translation: Analyzes protein synthesis but does not provide RNA localization data.
Conclusion
For detecting and visualizing RNAs crucial for Drosophila embryonic development, In situ hybridization (ISH) is the most appropriate technique due to its spatial specificity, high resolution, and ability to provide direct localization insights.



104 Comments
Shreeji Charan
March 1, 2025In situ hybridization
Suman bhakar
March 3, 2025Ok
Arushi
March 3, 2025👍👍
Kavita choudhary
August 20, 2025In situ hybridization
Prami Masih
March 4, 2025Done sir ✅
Aafreen
August 20, 2025Ans-3 In-Situ Hybridization
In-Situ Hybridization detect specific DNA or RNA sequence and labelled complementary probe to the RNA with in the cells
Northern blotting also detected RNA but lack of localisation
Sakshi yadav
August 20, 2025In situ hybridization
Priya choudhary
August 20, 2025Correct answer is in situ hybridization
Neelam Sharma
August 20, 2025In situ hybridization to detect RNA location in embryo
Neelam Sharma
August 20, 2025In situ hybridization
shruti sharma
August 20, 2025in situ hybridization
Mitali Saini
August 20, 2025For detecting and visualizing RNAs crucial for Drosophila embryonic development, In situ hybridization (ISH) is the most appropriate technique due to its spatial specificity, high resolution, and ability to provide direct localization insights.
Dipti Sharma
August 20, 2025In situ hybridization for detecting particular rna .
Manisha gujar
August 20, 2025In situ hybridisation
Manisha gujar
August 20, 2025Insitu hybridisation
Manisha gujar
August 20, 2025In situ hybridisation
Komal Soni
August 20, 2025In situ hybridization to detection perticular RNA in distinct region
Shivani
August 20, 2025In situ hybridization to detect perticular rna.
Option C is correct .
Mansukh Kapoor
August 20, 2025The correct answer is option 3rd
In situ hybridization tech.is the best for the identification of a particular RNA in drosophila
Khushi Mehra
August 20, 2025Answer is 3rd In situ hybridization
It is a technique for detection of specific RNA inside the cell or embryo by adding labelled DNA probe of a specific sequence.
Ekta yadav
August 20, 2025Insitu hybridisation
Varsha Tatla
August 20, 2025In situ hybridization because it is a most appropriate tech for detection of rna in developing drosophila embryo.it is a crucial tech due to the ability to provide specific locatization site, high resolution, special specity so option in situ hybridization is a best answer
Diksha Chhipa
August 20, 2025For rna in situ hybridisation
Aakansha Sharma
August 20, 2025In situ hybridization
Mohd juber Ali
August 20, 2025Option (3) right answer
In situ hybridization is appropriate teq. For detecting and visualisation Rna for drosophila embryo
Dharmpal Swami
August 20, 2025Ish technique use in drosophila for detecting perticular rna
Anurag Giri
August 20, 2025Ans 3 in situ hybridisation
Bcoz situ hybridization is the most appropriate technique specificity,and to provide direct localization insights.
Simran saini
August 20, 2025In situ hybridization.
HIMANI FAUJDAR
August 20, 2025Ans. In situ hybridization technique is suitable for detecting for drosophila embryonic development
Roopal Sharma
August 20, 2025Insitu Hybridization technique is used for detecting spatial rna localisation
Bharti Yadav
August 20, 2025In situ hybridization for detection of specific Rna sequence
Deepika Sheoran
August 20, 2025Option 3 is correct
Insitu hybridization to detect particular RNA.
Surbhi Rajawat
August 20, 2025Among options available both insitu hybridization and northern blotting can be the answer but the former is preferred because it is highly sensitive and requires less effort
Aman Choudhary
August 20, 2025Option 3 is correct because in-situ hybridization is used to detect the special Rna sequences
Surendra Dudi
August 20, 2025In situ hybridization
Mahima Sharma
August 20, 2025In situ hybridization tech . Best for detection of perticular rna
Nilofar Khan
August 20, 2025In situ hybridization
Avni
August 20, 2025In Situ Hybridization
Sneha .......
August 20, 2025In situ hybridization
Priya dhakad
August 20, 2025In situ hybridization is used for specific RNA.
anjani sharma
August 20, 2025In situ hybridization
Manisha
August 21, 2025In situ hybridization techniques used for detection RNAs crucial for drosophila development
Priyanka
August 21, 2025Done option 3
Neha
August 21, 2025Insitu hybridization as in this technique probe is directly given to the cell
Sakshi kanwar
August 21, 2025In situ hybridization
Arushi
August 21, 2025In situ hybridization
Sonam Saini
August 21, 2025Done
Santosh Saini
August 21, 2025In situ hybridization technique used for detection RNAs crucial for drosophila
Bhawna Choudhary
August 21, 2025In situ hybridization
Khushi Pareek
August 21, 2025In situ hybridization
Khushi Vaishnav
August 21, 2025In situ hybridization is the most appropriate technique
Shivani
August 21, 2025In situ hybridization 👍
Sakshi yadav
August 21, 2025Insitu hybridization
Minal Sethi
August 21, 2025In-Situ Hybridization
Divya rani
August 21, 2025In situ hybridization is the detection technique of specific rna inside the cell or drosophila embryo cell.
Sonam Saini
August 21, 2025Right is in situ hybridization because cell ke under embryo ke liye rna detect Krna h
Soniya Shekhawat
August 22, 2025In situ hybridization is a technique to use to detect the specific RNA or dna within tissues and embryo in drosphila development.
Sheikh Aleen
August 22, 2025In situ hybridization
Bhavana kankhedia
August 22, 2025In situ hybridization
Khushi Singh
August 22, 2025Ish is technique in which detection of particular rna inside cell which is suitable for embryonic devey
Kirti agarwal
August 23, 2025In situ hybridization
Khushi Agarwal
August 23, 2025Option 3 is correct answer
Insitu hybridization is detected location of embro
Priya khandal
August 23, 2025In situ hybridization
Niti Tanwar
August 23, 2025In situ hybridization
Jyoti
August 23, 2025In situ hybridization
karishma don
August 23, 2025ISH due to its high resolution and detecting exact location
Kanishka Parnami
August 23, 2025In situ hybridization allows us to locate RNA, gene location, duplication, deletion, translocation, inversion and many more.
Akshita Jangid
August 23, 2025In situ hybridization is a technique that is used to detect specific RNA sequences within the tissues
Samiksha bajiya
August 24, 2025In situ hybridization is a molecular technique used to detect specific RNA or DNA sequence within tissue section
Divya
August 24, 2025In situ hybridization is right amswer
Karishma
August 24, 2025In situ hybridization
Sonam Saini
August 24, 2025In situ hybridization
Kajal
August 24, 2025Correct answer is insitu Hybridization.
Yogita
August 24, 2025In Situ hybridization
Aakanksha Sharma
August 24, 2025In situ hybridization
Gunjan Singh
August 24, 2025in situ hybridization
Himanshi
August 24, 2025In situ hybridization
Anisha Beniwal
August 24, 2025In situ hybridization as we want to know about the region in the embryo
Meenakshi Choudhary
August 24, 2025In situ hybridization
Ayush
August 24, 2025Option c
In situ hybridization
B/s it Uses labeled RNA/DNA probes to visualize specific RNA molecules directly in tissue or embryos, showing where in the Drosophila embryo the RNA is localized
AKANKSHA RAJPUT
August 24, 2025In situ hybridization
Tanvi Panwar
August 24, 2025Insitu hybridization technique is used to locate or detect DNA in particular chromosomes.
Tanvi Panwar
August 24, 2025Insitu hybridization technique is used to locate or detect RNA in particular chromosomes.
Manisha choudhary
August 26, 2025Western blotting s protein kaa detection hota h
Northern blotting s rna kaa detection hota h y quantitative analysis k liye achha h pr Spatial resolution nhi deta h
In situ hybridization s single cell m bhi rna ki location pta kr kte h jo drosophila development k liye important hote h axis formation k liye
In situ hybridization Spatial resolution deta h
In vitro translation s protein formation pta chlta h
Neeraj Sharma
August 24, 2025Option C is right as in, in situ hybridization we can detect within the cell and northern blotting the detection is out of the cell so we cannot distinct the places
MOHIT AKHAND
August 24, 2025Done sir ✅
Pooja Sharma
August 24, 2025OPTION C IN SITU HYBRIDIZATION
Surendra Doodi
August 24, 2025In situ hybridization
Falguni Prakash
August 24, 2025Ish
Priyanka Meena
August 26, 2025In situ hybridization
Monika jangid
August 26, 2025In situ hybridization
Muskan Yadav
August 26, 2025Answer is 3rd – In situ hybridization
It is a technique for detection of specific RNA inside the cell or embryo by adding labelled DNA probe of a specific sequence.
Pratibha Jain
August 30, 2025Correct answer is option (c)
ISH is a technique to use to detect the specific RNA or dna within tissues and embryo in drosophila development.
Shriyanshi verma
August 30, 2025In situ hybridization
Alec
August 30, 2025In situ hybridization technique ismost suitable due it’s spatial stability, high resolution and ability to provide direct localization insights.
Asha Gurzzar
August 30, 2025In situ hybridization
Manisha choudhary
August 31, 2025Inse to hybridization
Manisha choudhary
August 31, 2025In situ hybridization
Anisha jakhar
August 31, 2025In situ hybridization
Kajal
August 31, 2025In situ hybridization
Shobha Kanwar
August 31, 2025In situ hybridization to detect RNA location in embryo
Kanica Sunwalka
August 31, 2025in situ hybridization – detect specific rna /dna seq and hybridize labelled complementary probe to target rna within cell
Anjali
September 1, 2025Insitu Hybridization technique is used for detecting spatial rna localisation
Deepika sheoran
November 15, 2025Insitu hybridization