DNA sequencing by the Sanger method takes advantage of which property of DNA synthesis to generate a sequencing ladder? A. Addition of nucleotides requires a free OH group on the […]
Tag: DNA Sequencing
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CSIR NET Previous Year Questions on DNA SEQUENCING
Understanding Protein Cleavage in Protein Sequencing
When sequencing proteins, one tries to generate overlapping peptides by using cleavages at specific sites. Which of the following statements about the cleavages caused by particular chemicals or enzymes is […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Understanding Amino Acid Variants in Sanger Sequencing
Representations of sequencing gels obtained from Sanger’s dideoxy chain termination method for variants A and B of the a chain of human hemoglobin are shown here. What is the nature […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Understanding Protease Activity and Its Mechanisms
Which of the following is not an example of protease activity? A. Some proteases cleave the phosphodiester bond between nucleic acid residues. B. Some proteases cleave within a protein sequence […]
CSIR NET Previous Year Questions on DNA SEQUENCING
How Frameshift Mutation Is Detected Using Dideoxy Sequencing
Figures A and B respectively represent the dideoxy sequencing gels obtained for partial sequences from 5′-ends of a bacterial gene and its mutant (with a point mutation) What type of […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Pyrosequencing Integration With PCR
Which of the following technique is not use in Pyrosequencing : A. Emulsion PCR B. Ligation based PCR C. Nick translation D. Inverse PCR Pyrosequencing Integration With PCR Pyrosequencing is […]
CSIR NET Previous Year Questions on DNA SEQUENCING
Examining Gene Expression Using DNA Microarrays
The sequencing of entire genomes has made it possible to examine the level of gene expression in a particular cell or tissue by using oligonucleotide probes to assess the level […]
CSIR NET Previous Year Questions on DNA SEQUENCING
How to Detect a Gene from an Amino Acid Sequence
If you know only the amino acid sequence of a protein, how could you detect the gene for that protein? A. You could screen a library of genes using a […]
CSIR NET Previous Year Questions on DNA SEQUENCING
DNA Sequencing Using Sanger’s Method
Understanding DNA Sequencing Using Sanger’s Method – Explained with an Example DNA sequencing is a critical technique in molecular biology that allows researchers to determine the exact order of nucleotide […]
CSIR NET Previous Year Questions on DNA SEQUENCING
tRNA Anticodon Base Pairing with mRNA Codons
The DNA sequence is ATG. What would be the sequence of bases in anticodon of tRNA A. CAU B. AUG C. UAC D. TAC Understanding tRNA Anticodon Base Pairing with […]
