Northern Hybridization: A Key Technique for RNA Detection
Northern hybridization is a technique for?
A. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
B. Detection of specific RNA sequences.
C. Detection of specific sequence among protein.
D. Eastern blotting
What Is Northern Hybridization Used For?
The correct answer is:
✅ B. Detection of specific RNA sequences.
Northern hybridization, also known as Northern blotting, is a laboratory technique used to detect and analyze specific RNA molecules in a complex mixture. It is a critical method for studying gene expression patterns in different tissues, developmental stages, or disease conditions.
Principle of Northern Hybridization
This technique works by separating RNA molecules by size, transferring them to a solid membrane, and then detecting specific RNA sequences using a labeled complementary probe.
Steps of Northern Hybridization
-
RNA Extraction:
- Total RNA or mRNA is isolated from biological samples.
-
Gel Electrophoresis:
- The RNA is separated based on size using agarose gel electrophoresis with formaldehyde to prevent secondary structures.
-
Transfer to Membrane:
- The RNA is transferred from the gel to a nylon or nitrocellulose membrane for hybridization.
-
Hybridization with a Probe:
- A radioactive, fluorescent, or enzyme-labeled DNA or RNA probe is introduced to bind to the target RNA sequence.
-
Detection:
- The probe hybridization is visualized using autoradiography or chemiluminescence.
Why Are the Other Options Incorrect?
❌ A. Detection of DNA Sequences – This describes Southern blotting, which is used for DNA analysis.
❌ C. Detection of Proteins – This describes Western blotting, which identifies proteins using antibodies.
❌ D. Eastern Blotting – Used for studying post-translational modifications of proteins, not RNA detection.
Conclusion
Northern hybridization is a crucial technique in molecular biology for detecting and analyzing specific RNA sequences. It plays a significant role in understanding gene expression patterns, making it valuable for research in disease mechanisms, drug discovery, and functional genomics.
This method is widely used in medical research, particularly in studying cancer, neurological disorders, and infectious diseases, by analyzing RNA expression differences between normal and diseased tissues. In drug discovery, it helps researchers evaluate how potential treatments affect gene expression.
While advanced techniques like qPCR and RNA sequencing (RNA-Seq) offer higher sensitivity and throughput, Northern blotting remains relevant due to its ability to provide visual confirmation of RNA integrity, size, and expression levels.
In summary, Northern hybridization remains a reliable tool in gene expression studies, contributing to major advancements in molecular biology, biotechnology, and medical research.
78 Comments
Suman bhakar
March 8, 2025👍👍
Mahima Sharma
August 21, 2025Detection of specific rna sequence
Akshay mahawar
March 17, 2025Done 👍
Nisha
March 17, 2025Done
Arushi
March 17, 2025👍👍
Parul
March 23, 2025Easily done sir.
Aakansha Sharma
August 20, 2025Detect of specific RNA sequence
Dipti Sharma
August 20, 2025Detection of specific RNA sequences.
shruti sharma
August 20, 2025option b
Anurag Giri
August 20, 2025Ans b
Detection of specific RNA sequences.
Diksha Chhipa
August 20, 2025Done
Khushi Mehra
August 20, 2025Northern blotting is used for detection of specific RNA sequence bands.
Nilofar Khan
August 20, 2025Correct answer is b
Detection of specific rna sequence
Dharmpal Swami
August 20, 2025Detection of specific rna sequence
Mansukh Kapoor
August 20, 2025The correct answer is option 2nd
Northern blotting is used to determine the specific RNA sequence
Bharti Yadav
August 20, 2025Detection of specific RNA sequences.
Sakshi yadav
August 20, 2025Detection of specific RNA sequence
Surbhi Rajawat
August 20, 2025Northern blotting is a technique used to detect specific RNA in a cell. And we can also estimate their amount and their molecular weight by this method
Priya dhakad
August 20, 2025Northern blotting is used for Detection of specific RNA sequences
anjani sharma
August 20, 2025Detection of specific RNA sequences.
Varsha Tatla
August 20, 2025Northern blotting is a tech for detection of specific rna on mem.
Kavita choudhary
August 20, 2025Detection of specific RNA sequence
Neelam Sharma
August 20, 2025Detection of specific RNA is Northern blotting
Deepika Sheoran
August 20, 2025Detection of specific RNA is Northern blotting.
Neha
August 21, 2025Northern hybridisation is a technique used to detect specific rna
Sneha .......
August 21, 2025Detection for specific rna sequence
Mohd juber Ali
August 21, 2025Northern blotting used to Detect of specific RNa sequence.
Aman Choudhary
August 21, 2025Northern blotting is used to detect the specific RNA sequences
Khushi Vaishnav
August 21, 2025NB -Detection of specific RNA sequences
Aafreen Khan
August 21, 2025Northern Blotting is used to detection of a specific RNA sequence
Avni
August 21, 2025B. Detection of specific RNA sequences
Simran Saini
August 21, 2025Detection of specific RNA sequences.
Bhawna Choudhary
August 21, 2025Detection of specific RNA sequence in northern blotting
Santosh Saini
August 21, 2025Detection of specific RNA seq.
Sakshi Kanwar
August 22, 2025B… detection of specific RNA sequence using DNA probe
Priyanka choudhary
August 22, 2025Right answer is option
Priyanka choudhary
August 22, 2025Right answer is option b,Detect of specific RNA sequence
Soniya Shekhawat
August 22, 2025Northern blotting is a detection of specific RNA sequenceby using dna probe
karishma
August 22, 2025Detection of specific RNA sequences.
Arushi
August 22, 2025Detection of specific RNA sequence
Kirti agarwal
August 23, 2025Detection of specific rna sequence
Priya khandal
August 23, 2025Detection of specific mrna
Akshita Jangid
August 23, 2025Northern hybridization is a technique for detection of specific RNA sequence
Kajal
August 23, 2025Northern hybridization is used for detection of specific rna sequence
Devika
August 24, 2025Detection of specific rna sequences
Yogita
August 24, 2025Detection of specific RNA sequence
Mitali saini
August 24, 2025B. Detection of specific RNA sequences.
Northern hybridization, also known as Northern blotting, is a laboratory technique used to detect and analyze specific RNA molecules in a complex mixture. It is a critical method for studying gene expression patterns in different tissues, developmental stages, or disease conditions.
Urmi sisodiya
August 24, 2025Northern blotting is responsible for seperation of rna. For dna, Southern blotting technique is used and for protein, western blotting technique is used.
AKANKSHA RAJPUT
August 24, 20252nd option is correct
Meenakshi Choudhary
August 24, 2025Detection of specific RNA sequences
Shivani
August 24, 2025Northern blotting is used for detection of specific RNA sequence
Ankita Pareek
August 24, 2025Detection of specific rna sequence is by Northern blotting
Bhavana kankhedia
August 24, 2025Detection of specific RNA sequences.
Anisha Beniwal
August 24, 2025Option B
Neeraj Sharma
August 24, 2025Option B northern blotting helps in separation and detection of specific RNa
Ayush Dubey
August 24, 2025Detection of specific RNA sequences
Himanshi
August 24, 2025Detection of specific rna sequence
Tanvi Panwar
August 24, 2025Northern blotting is a technique used for the detection of specific mRNA.
Sakshi yadav
August 24, 2025Detection of specific RNA sequence
Priyanka Verma
August 24, 2025northern blotting is a technique used for detection of specific RNA sequence
Surendra Doodi
August 24, 2025Detection of specific RNA sequences
Sonam Saini
August 24, 2025Specific m rna ka pta lagana
sakshi khatri
August 24, 2025detection of specific RNA
Falguni Prakash
August 24, 2025For specific rna sequence
Kajal
August 25, 2025Detection of specific rna sequence
Priyanka Meena
August 26, 2025Detection of specific RNA sequence
Muskan Yadav
August 26, 2025Detection of specific RNA sequences.
Manisha choudhary
August 27, 2025Detection of specific rna sequence
Kanica Sunwalka
August 28, 2025done
Komal Sharma
August 30, 2025Detection of specific RNA sequences.
Shriyanshi verma
August 30, 2025Detect of specific RNA sequence
Asha Gurzzar
August 30, 2025B is correct detection of specific rna sequence
Alec
August 30, 2025option b – detection of specific Rna sequences.
Pratibha Jain
August 30, 2025correct answer is option B
Detection of specific RNA sequences.
anjani sharma
August 30, 2025Northern blotting is used for detection of specific RNA sequence
Anisha jakhar
August 31, 2025Detection of specific RNA sequence
Shobha Kanwar
August 31, 2025The correct answer is option 2nd
Northern blotting is used to determine the specific RNA sequence
Anjali
September 1, 2025Detection of specific RNA sequences.