Zoo Blotting: Detecting Conserved DNA Sequences Across Species
A zoo blot helps to detect DNA sequences that
A. Are mutating at a fast rate.
B. Are conserved between species.
C. Are lost due to species extinction.
D. Are processed pseudogenes.
What Does a Zoo Blot Detect?
A zoo blot is a specialized form of Southern blotting that helps in identifying DNA sequences conserved across different species. The correct answer is:
✅ B. Are conserved between species.
How Does Zoo Blotting Work?
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DNA Extraction from Multiple Species:
- Genomic DNA is isolated from various organisms (e.g., humans, primates, rodents, birds, fish).
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Restriction Enzyme Digestion:
- The DNA is cut into fragments using restriction enzymes.
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Agarose Gel Electrophoresis:
- DNA fragments are separated based on size through electrophoresis.
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Transfer to a Nitrocellulose or Nylon Membrane:
- The DNA is blotted onto a membrane for further analysis.
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Hybridization with a Labeled Probe:
- A radioactive or fluorescent DNA probe specific to a gene of interest is applied.
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Detection:
- If a gene is evolutionarily conserved, the probe will hybridize to DNA from multiple species, appearing as a signal on an autoradiograph.
Why Is Zoo Blotting Important?
- Identifies evolutionarily conserved genes shared among species.
- Helps in comparative genomics and phylogenetics.
- Used to detect genes with important biological functions.
Why Are the Other Options Incorrect?
❌ A. Detects fast-mutating sequences – Zoo blotting identifies highly conserved, slowly evolving genes, not rapidly mutating ones.
❌ C. Detects extinct species’ DNA loss – Zoo blotting compares living species, not extinct ones.
❌ D. Detects processed pseudogenes – Pseudogenes often lack conservation; zoo blotting focuses on functional genes.
Conclusion:
Zoo blotting is a powerful comparative genomics tool, allowing scientists to identify conserved genes that play critical roles in evolution and biological functions across species.
68 Comments
Suman bhakar
March 3, 2025Ok sir
Ujjwal
March 3, 2025Done sir
Lokesh kumawat
March 4, 2025Attempted,now zoo blotting remember
Akshay mahawar
March 3, 2025Done 👍
Arushi
March 3, 2025Done👍
Deeksha Ojha
March 3, 2025Done sir
Priyam choudhary
March 3, 2025Ohk sir
Priya khandal
March 3, 2025Done Sir
Nisha
March 4, 2025Good explanation
Hemlata Meena
March 4, 2025Ok sir 👍
Prami Masih
March 4, 2025Okay sir
VIKRAM GAHLOT
March 7, 2025ok sir
Dipti Sharma
August 20, 2025Zoo bolta are conserved between species.
Dipti Sharma
August 20, 2025Zoo blots are conserved between species.
Khushi Mehra
August 20, 2025Zoo blotting is a type of southern blotting
It is used to identify specific DNA sequences that are conserved between species
Mansukh Kapoor
August 20, 2025The correct answer is option 2nd
Zoo blots helps to determine the genes conserved between different species
Aman Choudhary
August 20, 2025Zoo blots are conserved b/w species
Varsha Tatla
August 20, 2025Clear
Varsha Tatla
August 20, 2025Clear
Zoo plats are conserved seq. Between 2 spp.
Surbhi Rajawat
August 20, 2025Understood. Zoo blot is a special type of blotting technique to detect DNA across different species
Priya choudhary
August 20, 2025Correct answer is option b,are
conserved between specie
anjani sharma
August 20, 2025Zoo blots are conserved between species and help in studying evolutionary tree
Neelam Sharma
August 20, 2025DNA sequences Are conserved between species. Useful for evolutionary tree
Deepika Sheoran
August 20, 2025Zoo blots are conserved sequence between species.
Mohd juber Ali
August 21, 2025Option b
zoo blotting helps in identify dna sequence conserved accross different species
Khushi Vaishnav
August 21, 2025Are conserved between species.
Aafreen Khan
August 21, 2025Understand by video Zoo blot is a special type of blotting technique to detect DNA between two different species
Meera Gurjar
August 21, 2025Conserve between species
Avni
August 21, 2025B. Are conserved between species.
shruti sharma
August 22, 2025are conserve between species
Sakshi kanwar
August 22, 2025Option B DNA is conserved between species and is specific in each species
Soniya Shekhawat
August 22, 2025Remember zoo blot are detect to conserve sequence between species.
Simran Saini
August 22, 2025Are conserved between species.
Parul Yadav
August 22, 2025ZOO Blotting helps in identifying DNA sequences conserved across different species.
Arushi
August 22, 2025ZOO blot is used to detect DNA sequences which are conserved in species
Kirti agarwal
August 23, 2025Conserved between species
Priya dhakad
August 23, 2025Option b is correct conserved between species.
Priya khandal
August 23, 2025Are conserved between species
karishma don
August 23, 2025zoo blot is a type of southern blotting which identify the dna seq conserved across different species
Divya Mandhania
August 23, 2025Conserved between species
Bharti Yadav
August 23, 2025Option B DNA is conserved between species and is specific in each species
Kanishka Parnami
August 23, 2025Conserved sequence across diff species
Devika
August 24, 2025Conserved between species
Sneha Kumawat
August 24, 2025Zoo blot me different different species ke dna ko ek species ke dna probe ke sath hybrdize kiya jata h agar probe dusri species ke dna ke sath bhi judta h toh iska mtlbh yeh sequence Kai species me conserved h
Aakanksha Sharma
August 24, 2025Conserved between species
Himanshi
August 24, 2025Zoo blotings are conserved between species
Shivani
August 24, 2025Zoo blots helps to detect DNA sequence that are conserved between species .
Kajal
August 24, 2025Zoo blotting is a specialised form of Southern blotting detect dna sequences that are conserved across species….
Bhavana kankhedia
August 24, 2025zoo blots are help to detect DNA seq. conserved between species.
Ayush Dubey
August 24, 2025Are conserved between species
Priyanka Verma
August 24, 2025zoo blots are conserved between species
Sonam Saini
August 24, 2025Conserved sequence across different species
Surendra Doodi
August 24, 2025conserved between species
sakshi khatri
August 24, 2025b is correct
Falguni Prakash
August 24, 2025B
Swati Choudhary
August 24, 2025Zoo blot is a special type of blotting technique to detect DNA across different species.
Kajal
August 25, 2025Are conserved between two different species
Priyanka Meena
August 26, 2025Detecting conserved DNA sequences across species
Muskan Yadav
August 26, 2025Are conserved between species.
Manisha choudhary
August 27, 2025Zoo blot southern blotting jese hi h pr zoo blot m DNA different species s collect krte h and evolution k doran sbse jyada conserved evolutionary functional gene ki identify kiya jata h
Komal Sharma
August 30, 2025Zoo blotting is used for dna conserved sequence
Shriyanshi verma
August 30, 2025Zoo blots are conserved between species.
Alec
August 30, 2025zoo blot helps to detect dna sequences which are conserved between species.
Asha Gurzzar
August 30, 2025B is correct, zoo blot – conserved between two species
Pratibha Jain
August 30, 2025The correct answer is option (B)
Are conserved between species.
Shobha Kanwar
August 31, 2025Option b is correct as Zoo bolts are conserved between species.
Kanica Sunwalka
August 31, 2025zoo lot – conserved dna seq bw species
Anjali
September 1, 2025Conserved sequence across different species