Understanding Sanger Dideoxy Sequencing: Decoding DNA Sequences Sanger dideoxy sequencing is a widely used technique for determining the sequence of nucleotides in DNA. Developed by Frederick Sanger in 1977, […]
Tag: DNA Sequencing
Tag: DNA Sequencing
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Interpretation of Peptide Sequencing and Structural Modifications
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- March 16, 2025
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Interpretation of Peptide Sequencing and Structural Modifications An unknown peptide was isolated from the leaf of a medicinal plant and purified to homogeneity. The peptide did not yield any sequence […]
Sequence of Events in NGS-Based Whole Genome Sequencing
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- March 16, 2025
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Correct Sequence of Events in NGS-Based Whole Genome Sequencing Choose the correct sequence of events in a next generation sequencing technology-based whole genome sequencing project. A. DNA extraction shearing → […]
Pyrosequencing: Enzymes Involved and Their Functions
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- March 16, 2025
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Understanding Pyrosequencing and the Enzymes Involved Which of the following enzymes is NOT used in pyrosequencing? A. DNA Polymerase B. Pyrophosphatase C. Luciferase D. ATP sulfurylase Pyrosequencing is a widely […]
Solid Sequencing Technology and Its Key Enzyme
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- March 16, 2025
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Understanding Solid Sequencing Technology and Its Key Enzyme The enzyme used in Solid sequencing technology is : A. Sequenase B. DNA polymerase C. DNA Ligase D. Taq Polymerase Solid […]
SWISS-PROT: Features and Importance in Bioinformatics
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- March 16, 2025
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Understanding SWISS-PROT and Its Role in Bioinformatics Which statement does not go with SWISS-PROT technique? A. Provides a high level of annotations B. It is maintained by Swiss Institute of […]
Difference Between Shotgun Cloning and Clone-by-Clone Method
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- March 16, 2025
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How shotgun cloning differs from the clone-by-clone method A. The location of the clone being sequenced is known relative to other clones within a genomic library in the shotgun cloning. […]
The Role of Shotgun Sequencing in Human Genome Project
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- March 16, 2025
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Shotgun Sequencing: The Method Used to Sequence the Human Genome Which method was used to sequence the human genome? A. Cytogenetic mapping B. Shotgun sequencing C. Radiation hybrid mapping D. […]
Role of Dideoxynucleotides in Chain Termination Sequencing
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- March 16, 2025
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Which of the following components terminates the chain in a sequencing reaction? A. Dideoxynucleotides B. Klenow polymerase C. DNA polymerase Ill D. Deoxynucleotides Role of Dideoxynucleotides in Chain Termination […]


