Southern Blotting: The Technique Used in DNA Fingerprinting
Which of the following technique is used in DNA finger printing?
(a) Western blotting
(b) Southern blotting
(c) Northern blotting
(d) Eastern blotting
Correct Answer: (b) Southern Blotting
Southern Blotting in DNA Fingerprinting : DNA fingerprinting is a molecular technique used for identifying individuals based on unique DNA patterns. The correct technique used in DNA fingerprinting is Southern blotting, which detects specific DNA sequences.
Why Southern Blotting?
Southern blotting, developed by Edward M. Southern, allows the identification of specific DNA fragments within a mixture by using a radioactively or fluorescently labeled DNA probe. This technique is essential in DNA fingerprinting, where highly variable regions of DNA, such as VNTRs (Variable Number Tandem Repeats) and STRs (Short Tandem Repeats), are analyzed.
Why Other Options Are Incorrect?
- (a) Western Blotting – Used for protein detection, not DNA.
- (c) Northern Blotting – Detects RNA, not DNA.
- (d) Eastern Blotting – Primarily used for detecting post-translational modifications of proteins, not DNA.
Steps of Southern Blotting in DNA Fingerprinting
- DNA Extraction – DNA is isolated from a biological sample (blood, hair, saliva).
- Restriction Enzyme Digestion – DNA is cut into fragments using restriction enzymes.
- Gel Electrophoresis – DNA fragments are separated by size using agarose gel electrophoresis.
- Transfer to Membrane – DNA fragments are transferred onto a nylon or nitrocellulose membrane.
- Hybridization with Probe – A radioactive or fluorescent-labeled DNA probe binds to the target DNA sequence.
- Detection – The probe-bound DNA is visualized through autoradiography or chemiluminescence.
Applications of Southern Blotting in DNA Fingerprinting
- Forensic Science – Used for criminal investigations and solving crimes.
- Paternity Testing – Helps determine biological relationships.
- Genetic Studies – Used to identify genetic disorders and study hereditary diseases.
- Evolutionary Biology – Assists in studying genetic similarities and evolutionary relationships.
Conclusion
Southern blotting is the gold standard technique for DNA fingerprinting, making it an invaluable tool in forensic science, genetic testing, and evolutionary studies. Its ability to detect specific DNA sequences with high accuracy ensures its continued importance in molecular biology.
76 Comments
Priyanka yadav
April 1, 2025👍
Mahima Sharma
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April 9, 2025👍✔️
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Divya rani
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Aakansha Sharma
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Santosh Saini
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Dipti Sharma
August 20, 2025(b) Southern Blotting.
Asha Gurzzar
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Anurag Giri
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Mahima Sharma
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Diksha Chhipa
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Nilofar Khan
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anjani sharma
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Khushi Mehra
August 20, 2025Southern blotting is used for detection of specific DNA sequences and in DNA fingerprinting, individual can be identified based on specific DNA bands using VNTR
Mansukh Kapoor
August 20, 2025The correct answer is option 2nd
Southern blotting
Priya dhakad
August 20, 2025Southern blotting is correct answer
Kavita choudhary
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Priti khandal
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Komal Pareek
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Deepika Sheoran
August 21, 2025Option b is correct
Southern blotting.
Neha
August 21, 2025Southern blotting but difference is only in probe ,in dna finger print VNTR probe is used
Mohd juber Ali
August 21, 2025Southern blotting used in dna fingerprinting
Payal Gaur
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Parul Yadav
August 21, 2025The technique used in Fingerprinting is Southern Blotting.
Ankita Pareek
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Bharti Yadav
August 21, 2025Southern Blotting is Used in DNA Fingerprinting
Bhawna Choudhary
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Aafreen Khan
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Tanvi Panwar
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shruti sharma
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Aman Choudhary
August 22, 2025Southern blotting is used in DNA fingerprinting
Simran Saini
August 22, 2025Southern blotting.
Avni
August 22, 2025DNA fingerprinting by Southern Blotting
Priyanka choudhary
August 22, 2025Correct answer is option b,
Southern Blotting👍
Karishma
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Sakshi
August 22, 2025B is correct option
Dna fingerprinting is application of southern blotting
Kirti agarwal
August 23, 2025Southern blotting
Mitali saini
August 23, 2025Correct Answer: (b) Southern Blotting
Niti Tanwar
August 23, 2025B right answer
Southern blotting
Sakshi Kanwar
August 23, 2025Southern blotting used in identification of specific DNA fragments within a mixture by using a radioactive DNA probe.
Kajal
August 23, 2025Southern blotting associated with dna finger printing.
Yogita
August 24, 2025Southern blotting
Shobha Sharma
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Sneha Kumawat
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Shivani
August 24, 2025Southern blotting is used in dna fingerprinting
Neeraj Sharma
August 24, 2025When we detect vntr in southern blotting then it is known as DNA fingerprinting
Himanshi
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Himanshi
August 24, 2025Southern Blotting.
Pooja Sharma
August 24, 2025OPTION B SOUTHERN BLOTTING
Priyanka Verma
August 24, 2025Southern blotting is a technique for DNA fingerprinting based on VNTR
Falguni Prakash
August 24, 2025Southern blotting
Swati Choudhary
August 24, 2025Southern blotting is used in dna fingerprinting
Ajay Sharma
August 25, 2025southern it is
Kashish yadav
August 25, 2025Ok sir
Kajal
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Bhavana kankhedia
August 26, 2025Southern Blotting
Priyanka Meena
August 26, 2025Southern blotting
Anju
August 26, 2025Ans: b southern blotting
Detects specific DNA sequence
Muskan Yadav
August 26, 2025DNA fingerprinting – Southern blotting, which detects specific DNA sequences.
Manisha choudhary
August 27, 2025DNA fingerprinting is just like southern blotting
Pr DNA fingerprinting m VNTR yaa STR (short tendom repeats) probe use krte h
Kanica Sunwalka
August 28, 2025southern blotting with VNTR probe is dna fingerprinting
Komal Sharma
August 28, 2025The technique used in DNA fingerprinting is called Southern blotting
Shriyanshi verma
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Pratibha Jain
August 30, 2025Correct Answer is option (b)
Southern Blotting
Alec
August 30, 2025Southern blotting is used in Dna fingerprinting.
Anisha jakhar
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Shobha Kanwar
August 31, 2025Correct Answer: (b) Southern Blotting
Anjali
September 1, 2025Southern blotting