Detecting Viral Insertions in the Genome: The Role of Southern Blotting
The virus inserted in genome can be recognized by
A. FISH
B. Northern blot
C. Microarray
D. Southern blot
Which Technique Detects a Virus Inserted into the Genome?
When a virus integrates its DNA into a host genome, detecting its presence is crucial for understanding viral infections, gene therapy, and disease progression. The correct answer is:
✅ D. Southern Blot
Southern blotting is a DNA hybridization technique used to detect specific DNA sequences, including viral DNA integrated into the host genome.
How Does Southern Blotting Detect Viral Insertions?
-
Genomic DNA Extraction:
- The host cell’s DNA is isolated from the tissue or cultured cells.
-
Digestion with Restriction Enzymes:
- DNA is cut into fragments using restriction enzymes to analyze specific sequences.
-
Agarose Gel Electrophoresis:
- The DNA fragments are separated based on size through gel electrophoresis.
-
Denaturation:
- The double-stranded DNA is denatured into single strands using an alkaline solution (NaOH).
-
Transfer to a Nitrocellulose or Nylon Membrane:
- The DNA is transferred onto a solid membrane via capillary or vacuum transfer.
-
Hybridization with a Labeled Probe:
- A radioactive or fluorescently labeled DNA probe complementary to the viral DNA sequence is applied.
-
Detection:
- The hybridized probe reveals the presence and location of viral DNA in the genome.
Why Are the Other Options Incorrect?
❌ A. FISH (Fluorescence In Situ Hybridization)
- Detects chromosomal abnormalities and large genetic insertions, but is less effective for viral integration analysis.
❌ B. Northern Blot
- Used for detecting RNA, not genomic DNA insertions.
❌ C. Microarray
- Used for gene expression analysis, not for detecting specific viral DNA integration.
Conclusion:
Southern blotting remains the gold standard for detecting viral DNA insertions into the host genome. It is widely used in virology, gene therapy, and molecular diagnostics to study retroviral integrations, transgene stability, and genetic diseases.
90 Comments
Suman bhakar
March 3, 2025Sir video mein to FISH correct answer bta rhe h sir
Jyoti Meena
March 3, 2025Done
Akshay mahawar
March 3, 2025Done👍
Arushi
March 3, 2025👍done
Arushi
March 3, 2025done👍
Deeksha Ojha
March 3, 2025Done
Abhilasha
March 3, 2025Done ✅
Priyam choudhary
March 3, 2025Done 👍
Priyam choudhary
March 3, 2025Done sir
Priti khandal
March 3, 2025Done Sir 👍
Nisha
March 4, 2025which answer is correcto
A or D
Nisha
March 4, 2025which answer is correct
Prami Masih
March 4, 2025FISH technique is right sir 👍
Mahima Sharma
August 21, 2025Fish tec. Vese ans alg h
Jonu Meena
March 4, 2025👍🏻👍🏻
Aakansha Sharma
August 20, 2025Fish tech.
Lokesh kumawat
March 4, 2025Done
Lokesh kumawat
March 4, 2025I think Fish is right.
but virus genomic material me dna & rna Kuch bhi ho skta hai na aur mostly virus me toh rna hota hai toh written explanation k according toh northern blotting bhi ho skta hai na q nahi
shivani meena
March 6, 2025done
VIKRAM GAHLOT
March 7, 2025👍
Khushi Vaishnav
August 19, 2025Southern Blot
Neelam Sharma
August 19, 2025D. Southern blot
Dipti Sharma
August 20, 2025D. Southern Blot
Khushi Mehra
August 20, 2025Correct answer is FISH because virus DNA is inserted in genome and thus in-vivo method is used for detection
Khushi Mehra
August 20, 2025FISH is correct…. because viral RNA is inserted in genome which can’t be detected by southern blotting and within the cell we have to identify so in vivo method is used
shruti sharma
August 20, 2025southern blotting
Aafreen
August 20, 2025Ans- 4
Southern blot which technique detects virus inserted into the genome
Dharmpal Swami
August 20, 2025Southern blotting
Nilofar Khan
August 20, 2025Southern blotting
Mansukh Kapoor
August 20, 2025The answer can be FISH and southern blotting
Sakshi yadav
August 20, 2025Southern blotting
Aman Choudhary
August 20, 2025Southern blotting
Varsha Tatla
August 20, 2025Southern blotting n fish
Priya choudhary
August 20, 2025Right answer is option d, Southern blotting
Priya dhakad
August 20, 2025FISH and southern blotting
anjani sharma
August 20, 2025FISH and southern blotting
Aafreen
August 20, 2025Ans- 4
Southern blot which technique detects virus inserted into the genome
Kavita choudhary
August 20, 2025Sourthan blotting
Neelam Sharma
August 20, 2025Southern blotting
Deepika Sheoran
August 20, 2025Southern blotting
Sneha .......
August 21, 2025FISH and southern blotting
Payal Gaur
August 21, 2025Virus inserted in genome can be recognize by southern blotting technique
Mohd juber Ali
August 21, 2025Southern blotting used to detect specific dna sequence (viral dna insertion
Manisha
August 21, 2025Southern blotting used to detect specific dna sequence
Santosh Saini
August 21, 2025Southern blotting used to detect specific DNA including viral genomic DNA and could also be FISH
Meera Gurjar
August 21, 2025Southern blotting
Simran Saini
August 21, 2025Southern blot.
Avni
August 21, 2025Southern blot
Divya rani
August 21, 2025Detection of viral dna is the application of Southern blotting.
Sakshi Kanwar
August 22, 2025Option D
Soniya Shekhawat
August 22, 2025Southern blotting is a gold standard for detecting the viral DNA insertion into host genome.
karishma
August 22, 2025Southern blotting remains the gold standard for detecting viral DNA insertions into the host genome.
Parul Yadav
August 22, 2025Southern Blotting is used to detect Specific DNA sequences, including viral DNA integrated into Host genome.
Arushi
August 22, 2025FISH
Kirti agarwal
August 23, 2025Southern blotting
Priya khandal
August 23, 2025Southern blotting
Ankita Pareek
August 23, 2025Southern blotting is technique used to detect viral dna insertion
Akshita Jangid
August 23, 2025Southern blotting is a technique that is used to detect the viral DNA insertion into host genome
Divya Mandhania
August 23, 2025Confused between fish and southern blotting
Bharti Yadav
August 23, 2025FISH is best method to detect virus in genome
Devika
August 24, 2025I think fish but the given correct answer is Southern blotting in this explanation video sir also tell fish is the Right answer
Sneha Kumawat
August 24, 2025Fish me flourocent prob ka use Kiya jatah jo viral DNA ke complimentary sequence se jud jata h
Yogita
August 24, 2025Fish
Himanshi
August 24, 2025Fish
Urmi sisodiya
August 24, 2025FISH is correct bcoz mostly virus contain rna and for detection of particular rna inside cell FISH technique is used
AKANKSHA RAJPUT
August 24, 2025FISH
Shivani
August 24, 2025FISH technique can be used to detect virus insert in genome
Kajal
August 24, 2025Southern blotting
Bhavana kankhedia
August 24, 2025Southern Blot
Ayush Dubey
August 24, 2025Southern blotting
Tanvi Panwar
August 24, 2025Southern blotting is a technique for detection of specific DNA including viral genome.
Priyanka Verma
August 24, 2025FISH is the best technique to detect the virus inserted in the genome
Ankush
October 18, 2025Please contact is you have given sci b interview recently
Sonam Saini
August 24, 2025Southern bloting
Sonam Saini
August 24, 2025Fish technic
Surendra Doodi
August 24, 2025Southern blotting is a DNA hybridization technique used to detect specific DNA sequences
sakshi khatri
August 24, 2025southern blot
Falguni Prakash
August 24, 2025A
Swati Choudhary
August 24, 2025southern blotting
Kajal
August 25, 2025Southern blotting
Priyanka Meena
August 26, 2025Southern blotting
Manisha choudhary
August 27, 2025Vital DNA infection k liye southern blotting ki jaati h gene therapy,viral progression ,disease diagnosis k liye southern blotting useful h
Fish chromosomal aberration,
Large generic insertion k liye hota h
Microarray gene expression detection k liye hota h
Komal Sharma
August 30, 2025Fish technique is used.
Komal Sharma
August 30, 2025Southern blotting is a DNA hybridization technique used to detect specific DNA sequences, including viral DNA integrated into the host genome. I think this one is most suitable answer
Shriyanshi verma
August 30, 2025Correct answer is FISH because virus DNA is inserted in genome and thus in-vivo method is used for detection
Alec
August 30, 2025The virus inserted in genome is detected by southern blotting.
Asha Gurzzar
August 30, 2025Southern blotting is for virus dna detection,but FISH is used here because virus inject in genome
Pratibha Jain
August 30, 2025The correct answer is option (D)
Southern Blot
Shobha Kanwar
August 31, 2025The correct answer is FISH
Anjali
September 1, 2025Southern bloting