Interpreting Southern Blot Results
Isolated variety of E .coli is followed by southern blot procedure. A radio active probe is then hybridized with gene of interest & observed single band of DNA after probing. What are your assumption on the basis of observation?
A. All of the clones tested, except the one described, have the gene interest.
B. Only the one clone described has the gene of interest.
C. The part of the gene recognized by the probe is located on the DNA band visualized.
D. Both B and C
Interpreting Southern Blot Results: Understanding DNA Hybridization in E. coli
Correct Answer: (D) Both B and C
Southern blotting is a powerful molecular biology technique used to detect the presence of specific DNA sequences within a complex mixture. In this scenario, an isolated E. coli variety was subjected to the Southern blot procedure, followed by hybridization with a radioactive probe targeting a gene of interest. The observation of a single DNA band after probing leads to the following conclusions:
Interpreting Southern Blot Results
-
Only One Clone Contains the Gene of Interest
- The Southern blot technique detects specific DNA sequences based on complementary base pairing between the radioactive probe and the target DNA.
- Since only one clone produces a visible band, it indicates that only this clone contains the gene of interest, while others do not.
- This supports statement (B): “Only the one clone described has the gene of interest.”
-
The Probe Recognizes a Specific DNA Segment
- The single band observed confirms that the gene fragment recognized by the probe is present on that DNA band.
- This means the target sequence hybridized specifically to the labeled probe, which is critical for verifying gene presence and structure.
- This supports statement (C): “The part of the gene recognized by the probe is located on the DNA band visualized.”
Why Other Options Are Incorrect?
- Option A: This statement suggests that all clones except one contain the gene of interest, but the results indicate that only one clone hybridized with the probe. Hence, this is incorrect.
Conclusion
From the Southern blot observation, we can conclude that only one E. coli clone carries the gene of interest, and the probe specifically hybridized with a part of the gene located on the DNA band visualized. Thus, the correct answer is (D) Both B and C.
85 Comments
Shreeji Charan
March 1, 2025🙋♀️
Ayush
August 24, 2025Option d
Southern blot uses a probe to detect if a specific DNA sequence is present.
If a single band is seen, it means:
Only one clone contains that gene sequence
The band that appears is exactly where the probe hybridized
Suman bhakar
March 3, 2025👍👍
Arushi
March 3, 2025👍👍
Prami Masih
March 4, 2025Done sir
Sakshi yadav
August 20, 2025Done 👍🏻
Aafreen
August 20, 2025Both B and C are correct
B:- one clone produces a visible band it indicates that only this clone contains the gene of interest
C:- The part of the gene recognized by the probe is located on the DNA band visualized
Neelam Sharma
August 20, 2025Both B and C
Mitali Saini
August 20, 2025Correct Answer: (D) Both B and C
Dipti Sharma
August 20, 2025The correct answer is (D) Both B and C.
Manisha gujar
August 20, 2025Both c and d is correct
Manisha gujar
August 20, 2025Option d is correct
Komal Soni
August 20, 2025Both b and c
Shivani
August 20, 2025Option D. ( Both b and c ) is correct .
Mansukh Kapoor
August 20, 2025The correct answer is option 4th
Both B and C
Mahima Sharma
August 20, 2025Both b and c
Khushi Mehra
August 20, 2025Both B and C statement are correct.
Only cloned DNA have the gene of interest shown by a band
And the labelled probe recognise the part of gene and get bind to specific DNA band
Ekta yadav
August 20, 2025Option. B & C
Aakansha Sharma
August 20, 2025Both B and C
Diksha Chhipa
August 20, 2025Both b nd c are right
Mohd juber Ali
August 20, 2025Option (D)
: southern blotting used to detect specific DNa sequence
Radio label probe binds with only particular Dna segment
Anurag Giri
August 20, 2025Ans d is correct
E. coli clone carries the gene of interest, and the probe specifically hybridized with a part of the gene Located on the dna band
Bharti Yadav
August 20, 2025Both b and c are right.
Roopal Sharma
August 20, 2025Probe specifically binds with a part of gene and visualized dna band can be seen
Surbhi Rajawat
August 20, 2025In southern blot if we see a band that means our probe has bind and we can detect our desired DNA sequence. Hence option D is correct
Aman Choudhary
August 20, 2025D is correct
Clone contain the gene of interest and probe is present on dna band
Surendra Dudi
August 20, 2025Option D
Deepika Sheoran
August 20, 2025Correct answer is option 4th
Both B&C
Nilofar Khan
August 20, 2025Both B and C
Avni
August 20, 2025D) Both B and C
Bhawna Choudhary
August 23, 2025Both And B
Payal Gaur
August 20, 2025B and C correct in southern blotting technique DNA detect and band show, probe is used for particular band
Priya dhakad
August 20, 2025Both b and c
Only the one clone described has the gene of interest.
The part of the gene recognized by the probe is located on the DNA band visualized.
anjani sharma
August 20, 2025B and c
Kavita choudhary
August 20, 2025B and c
Manisha
August 21, 2025Southern blotting only one alone contains the gene of interest, specific dna sequence
Sneha .......
August 21, 2025Both b and c is correct
Neha
August 21, 2025Both b and c statement
Divya rani
August 21, 2025B amd C both because only one clone has the gene of interest and and Southern blotting is specific to dna .
Sonam Saini
August 21, 2025Both B and c
Santosh Saini
August 21, 2025Both B and C statement are currect bcz southern blotting is used for detection of specific DNA and labeled probe binds with specific DNA
Khushi Pareek
August 21, 2025Both b and c
Southern blot: detection of specific DNA
Karishma
August 21, 2025Option b and c is right
Khushi Vaishnav
August 21, 2025E. coli clone carries the gene of interest, and the probe specifically hybridized with a part of the gene located on the DNA band visualized.
Sakshi Kanwar
August 21, 2025Option D
As Southern blotting is used to detect specific DNA sequences using a designed radioactive probe which show only one clone as mentioned presence of one band
Simran Saini
August 21, 2025Both b and c is right option.
Soniya Shekhawat
August 22, 2025Clone contain gene of interest and probe is LOCALISED for specific DNA sequence so D IS CORRECT.
Bhavana kankhedia
August 22, 2025Both b and c is right option
Khushi Singh
August 22, 2025Both b and c
Kirti agarwal
August 23, 2025Opt d
Khushi Agarwal
August 23, 2025Option d is correct
Matlab ek hi clone mein gene of interest hai (B).
Aur wo band gene ke uss part ko represent karta hai jisme probe bind hua hai (C)
So answer is = B + C (Option D).
Priya khandal
August 23, 2025B and c done sir
karishma don
August 23, 2025both b and c
Akshita Jangid
August 23, 2025Both B and C Option D is right
Karishma
August 24, 2025Both B and c is correct
Sheikh Aleen
August 24, 2025Both b and c
Yogita
August 24, 2025Both b&c
Gunjan Singh
August 24, 2025correct answer will be both B and C
Himanshi
August 24, 2025B and c both
Anisha Beniwal
August 24, 2025Option d is correct
Meenakshi Choudhary
August 24, 2025Both b and c
AKANKSHA RAJPUT
August 24, 2025B and c
Tanvi Panwar
August 24, 2025Only one E.coli clone carries the gene of interest and probe specifically with the part of the gene located on the DNA band visualized.
Neeraj Sharma
August 24, 2025Both B and C are correct here
Kajal
August 24, 2025Option d which include both b n c
shruti sharma
August 24, 2025Option b and c
Clone me gene inserted h
Aur vo band gene ke uss part pe represent krta h jisme probe bind h
Sonam Saini
August 24, 2025Right answer B and C
MOHIT AKHAND
August 24, 2025Done sir ✅
Pooja Sharma
August 24, 2025BOTH B AND C OPTIONS ARE CORRECT
Surendra Doodi
August 24, 2025one clone produces a visible band it indicates that only this clone contains the gene of interest
C:- The part of the gene recognized by the probe is located on the DNA band visualized
Falguni Prakash
August 24, 2025D
Priyanka Meena
August 26, 2025Only the one clone described has the gene of interest.
The part of the gene recognized by the probe is located on the DNA band visualized.
Manisha choudhary
August 26, 2025Answer D
B and c both statement correct
Statement a k according ek clone ko chhodkr baaki sbhi cone k paas gene of interest h y wrong statement h kyoki ek cone s single band observe ho rha h mtlb ek cone k paas hi gene of interest h
Statement second k according ek clone k paas gene of interest h y right statement h kyoki ek cone s hi band aa rha h
Statement c right h kyoki probe gene of interest k complimentary hota h jo gene of interest s jaakr bind krta h
Single band y bta rha h ki specific binding hui h DNA k paas gene of interest h
Muskan Yadav
August 26, 2025B and c both are correct answer
Nikita Saini
August 28, 2025Yes sir
Done ✅
Pratibha Jain
August 30, 2025Correct answer is option (d) both b and c
only one E. coli clone carries the gene of interest, and the probe specifically hybridized with a part of the gene located on the DNA band visualized.
Shriyanshi verma
August 30, 2025Both c and d is correct
Alec
August 30, 2025Both b and c are correct options.
Asha Gurzzar
August 30, 2025Both b and c are correct
Anisha jakhar
August 31, 2025Both b and c
Kajal
August 31, 2025Correct Answer is (D) Both B and C
Shobha Kanwar
August 31, 2025Both B and C both bcoz only one clone has the gene of interest and and Southern blotting is specific to dna .
Kanica Sunwalka
August 31, 2025both b and c are correct
only 1 e.coli clone carries the gene of interest and probe bind specifically with the part of gene located on dna band visualized
Manisha choudhary
September 1, 2025(D) Both B & C
Deepika sheoran
November 15, 2025Option b& c is correct