1. In one study, a group of 5 day rat pups were fed for 3 weeks a diet A and the pups gained weight by 300%. In a second study, when the same diet fed for 3 weeks to rats of 350 gms, they did not gain weight significantly. In a third study, a diet B was fed to 250-350 gms rats and it was observed that they delivered normal pups after five weeks. Based on these observations which of the following statements is correct?
    (1) Diet A facilitates weight gain than diet B.
    (2) Diet B facilitates pregnancy and child-bearing.
    (3) More control experiments are to be conducted for definitive conclusion.
    (4) Diet A is more energy containing that diet ‘B’. Hence, its quantity should be reduced.


    Analysis of the Observations

    In the described studies:

    • Study 1: Five-day-old rat pups fed diet A for three weeks showed a remarkable 300% weight gain, demonstrating high growth potential in neonatal rats with adequate nutrition.

    • Study 2: Adult rats weighing around 350 gms fed the same diet A for three weeks did not exhibit significant weight gain, indicating age-related differences in metabolism and growth potential.

    • Study 3: Rats weighing 250-350 gms fed diet B for five weeks successfully became pregnant and delivered normal pups, suggesting diet B supports reproductive capability and offspring development.

    Understanding the Results

    • The neonatal rats are in a rapid growth phase requiring high energy and nutrients, which diet A seems to provide effectively, leading to substantial weight gain.

    • Adult rats, being closer to physiological maturity, have lower energy needs for growth and instead primarily expend energy on maintenance. Hence, diet A did not result in weight gain, pointing out that the diet’s energy content or composition suits rapid growth phases better.

    • Diet B appears adequate or optimized to support reproductive health rather than promote excess weight gain in adult rats, facilitating successful pregnancy and healthy pups.

    Correct Interpretation

    From these observations,

    • It cannot be conclusively said that diet A facilitates more weight gain than diet B in adult rats since weight gain was minimal in adult rats on diet A.

    • Diet B facilitates pregnancy and childbearing based on study 3, showing evidence of fertility and normal litter delivery.

    • More control experiments to compare energy content, nutrient balance, and physiological effects of both diets would help to draw definitive conclusions.

    • Suggesting reduction in quantity of diet A based solely on these results without nutrient analysis is premature.

    Thus, the most reasonable and evidence-supported conclusion is:

    (3) More control experiments are to be conducted for definitive conclusion.

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